FastQCFastQC Report
Thu 26 May 2016
SRR1547338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547338_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2111939
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560232.65268078292034No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT29580.1400608635003189No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT23610.11179300159711052No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA207.0346316E-445.036
CGTAACG207.0346316E-445.01
CGTTTTT273400.044.160571
CGACGGT1900.042.6315828
CGCCGGT1700.039.70588328
CACGACG2050.039.51219626
TACGACG403.4600635E-739.3751
GTTTTTT311950.039.027892
CGGACGG750.039.0000042
TACGGGA8550.038.684214
TAGGGAC23850.037.7358475
GCGATCG1800.037.59
ACGGGAT9950.037.3115585
TAGGGAT31000.037.233875
ATAGGGA29900.037.1739124
TTAGGGA26700.036.8258444
CCGCTCG800.036.562519
GTAACGG1600.036.56252
TAGGGTA7900.036.4556965
GTACGGG7100.036.4436653
Produced by FastQC (version 0.11.5)