FastQCFastQC Report
Thu 26 May 2016
SRR1547337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547337_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1544137
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111530.7222804712276177No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG1000.045.01
GCCGATT253.8914302E-545.09
GTCGTAA207.0340396E-445.027
GCGATCG253.8914302E-545.09
GTATGCG900.042.51
CGTTTTT54450.042.1074371
CGTTAGG2800.041.7857172
TACGGGA8200.040.3353654
GCGCGAC1900.040.2631579
TAGGGAT24300.039.8148165
ACGGGAT8600.039.767445
TCACGAC2500.039.60000225
ACGGGAC6100.039.4672135
TAGGGTA6450.039.069775
TATAGCG700.038.571431
CGCAATC356.249684E-638.5714338
GTACGAT356.249684E-638.5714310
TAGGGAC20500.038.414635
ATAGGGC5900.038.1355934
AGGGATT29200.038.065076
Produced by FastQC (version 0.11.5)