FastQCFastQC Report
Thu 26 May 2016
SRR1547336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547336_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1192579
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293202.4585373379876723No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22430.18807978339380452No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13300.11152301021567544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCAAT351.2119744E-745.00000419
TCGTTTC207.0333935E-445.018
AATCCGA207.0333935E-445.037
ATTAGCG502.1827873E-1145.01
TTTACGG850.045.02
CGACGCG207.0333935E-445.038
CGACGAT207.0333935E-445.035
ACCGCGT207.0333935E-445.015
GGCGTAA406.8157533E-945.08
TAAGTCG207.0333935E-445.01
ATAGACG207.0333935E-445.01
ACTTACG453.8562575E-1045.01
CTACGCT207.0333935E-445.033
TTGCGAA253.890894E-545.01
GAACGCG207.0333935E-445.01
TCGTAAG406.8157533E-945.01
CGTAAGC253.890894E-545.020
TATAGCG850.045.01
CCGAATC207.0333935E-445.019
TCGACTC207.0333935E-445.038