##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547336_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1192579
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.254258208470887	31.0	31.0	33.0	30.0	34.0
2	31.648508819960774	31.0	31.0	34.0	30.0	34.0
3	31.59003554481506	31.0	31.0	34.0	30.0	34.0
4	35.4574673879047	37.0	35.0	37.0	33.0	37.0
5	35.381328197125725	37.0	35.0	37.0	33.0	37.0
6	35.43663438648509	37.0	35.0	37.0	33.0	37.0
7	35.89697537857031	37.0	35.0	37.0	35.0	37.0
8	35.89599515000683	37.0	35.0	37.0	35.0	37.0
9	37.62580340589596	39.0	37.0	39.0	35.0	39.0
10	36.8590248528609	39.0	37.0	39.0	32.0	39.0
11	36.567280658136696	39.0	35.0	39.0	32.0	39.0
12	36.111098719665534	38.0	35.0	39.0	32.0	39.0
13	35.879774002393134	38.0	35.0	39.0	31.0	39.0
14	36.97945041795973	39.0	35.0	41.0	31.0	41.0
15	37.1654573826975	39.0	35.0	41.0	32.0	41.0
16	37.24809257919182	39.0	35.0	41.0	32.0	41.0
17	37.16674367064991	39.0	35.0	41.0	32.0	41.0
18	36.961015580519195	39.0	35.0	40.0	32.0	41.0
19	36.79982709740822	38.0	35.0	40.0	31.0	41.0
20	36.52927646721936	38.0	35.0	40.0	31.0	41.0
21	36.381725655071904	38.0	35.0	40.0	31.0	41.0
22	36.30116327723363	38.0	35.0	40.0	31.0	41.0
23	36.2296921210251	37.0	35.0	40.0	31.0	41.0
24	36.06200511664217	37.0	35.0	40.0	31.0	41.0
25	35.9694653352105	37.0	35.0	40.0	30.0	41.0
26	35.796301964062756	37.0	35.0	40.0	30.0	41.0
27	35.69081628973846	37.0	34.0	40.0	30.0	41.0
28	35.687572060215714	37.0	34.0	40.0	30.0	41.0
29	35.65680680273592	37.0	34.0	40.0	30.0	41.0
30	35.52783673031304	36.0	34.0	40.0	29.0	41.0
31	35.31716976401563	36.0	34.0	40.0	29.0	41.0
32	35.045373933299174	36.0	34.0	40.0	27.0	41.0
33	34.66564898426016	36.0	34.0	40.0	24.0	41.0
34	34.277984938523986	36.0	33.0	40.0	23.0	41.0
35	33.98289756904993	36.0	33.0	40.0	21.0	41.0
36	33.77401580943485	36.0	33.0	40.0	20.0	41.0
37	33.63188518328765	36.0	33.0	40.0	18.0	41.0
38	33.56651928299928	36.0	33.0	40.0	18.0	41.0
39	33.55371677683407	36.0	33.0	40.0	18.0	41.0
40	33.405585709625946	36.0	33.0	40.0	18.0	41.0
41	33.35038433512581	36.0	33.0	40.0	18.0	41.0
42	33.26990413213716	35.0	33.0	40.0	18.0	41.0
43	33.20290312004488	35.0	33.0	40.0	18.0	41.0
44	33.04594244909561	35.0	32.0	40.0	17.0	41.0
45	32.96694390895698	35.0	32.0	40.0	17.0	41.0
46	32.88379553891189	35.0	32.0	40.0	17.0	41.0
47	32.7299851833715	35.0	32.0	40.0	15.0	41.0
48	32.67841375707605	35.0	32.0	40.0	15.0	41.0
49	32.57588050770641	35.0	31.0	39.0	15.0	41.0
50	32.472176685988934	35.0	31.0	39.0	15.0	41.0
51	32.058731538958845	35.0	31.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	3.0
13	8.0
14	19.0
15	49.0
16	133.0
17	326.0
18	789.0
19	1565.0
20	2696.0
21	4379.0
22	6604.0
23	9980.0
24	14880.0
25	22093.0
26	30344.0
27	32652.0
28	32070.0
29	32583.0
30	35816.0
31	41382.0
32	49861.0
33	62626.0
34	98197.0
35	141027.0
36	104688.0
37	106149.0
38	143397.0
39	218209.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.472411471273603	24.148756602287982	27.22804946255133	20.150782463887086
2	36.17630362433013	24.307152817549195	27.481198310552173	12.035345247568504
3	25.27849308096151	23.995810759706483	38.46680178000786	12.258894379324136
4	22.919068673857247	26.069048675182106	38.13592223240557	12.87596041855508
5	20.13057415902846	29.846408497885673	37.43181793407397	12.591199409011896
6	18.786512256211118	38.21633619240319	33.36533680368344	9.631814747702249
7	80.92503725120098	3.6205567933025815	13.91991641643866	1.53448953905779
8	81.73244707478499	2.951251028233769	13.441457547047197	1.8748443499340506
9	77.93798146705585	4.8093250006917785	14.91230350358341	2.3403900286689603
10	40.535427841677574	23.5657344293334	23.705599377483587	12.193238351505435
11	32.068399661573785	25.45583982277065	27.205157897296534	15.270602618359035
12	29.684322799579732	22.719752737554494	31.84451512226863	15.751409340597142
13	19.92211836700126	31.704566322231067	32.71380763873923	15.659507672028436
14	15.936302752270501	34.79895252222285	33.69931887111881	15.565425854387843
15	15.525344652220104	27.8789078123965	41.80980882608196	14.785938709301439
16	17.682937566400213	25.256272330805757	41.53863182229437	15.522158280499657
17	17.721006323270828	27.723111005644068	31.535353213497807	23.020529457587298
18	18.93308535535172	29.559802746820125	35.4125806340712	16.09453126375695
19	22.8675836150058	31.60193161207769	29.33382191033047	16.19666286258604
20	23.49991069773994	28.54796202180317	31.32513653183563	16.626990748621264
21	20.263814808075608	30.78663971107994	33.079988830928606	15.869556649915856
22	20.762230426663557	27.42761695451622	31.711609880771004	20.09854273804922
23	17.319607338381775	31.311720229854796	31.695510318394	19.67316211336943
24	18.13531849881643	28.292465320955678	37.000232269728045	16.57198391049985
25	17.47817125741775	29.84112582898072	34.58261465278191	18.09808826081962
26	16.101742526071646	33.555345180486995	31.34165535364953	19.00125693979183
27	16.133270835726606	34.30254934893202	32.37529756938534	17.188882245956034
28	15.511089831365469	31.639161850074505	36.04733942153937	16.80240889702066
29	15.732207258387076	29.70578888274907	35.88885935439078	18.67314450447308
30	17.278352209790715	32.52438622514735	33.38571281231684	16.8115487527451
31	21.045649806008658	30.584892070043157	30.603339485266805	17.766118638681377
32	21.235825886586966	30.82437306040103	31.625577844318908	16.314223208693093
33	20.71409944330732	31.531160619128794	29.42362728171467	18.331112655849214
34	17.516994681274785	32.21891379942126	31.086410208464176	19.177681310839784
35	16.785554667657237	31.7764273897159	31.597655165821298	19.840362776805563
36	22.278188698610325	32.086511669247905	27.838239647017094	17.797059985124676
37	17.041470627941628	35.50364378376611	29.851355759241105	17.603529829051155
38	17.746161889484892	35.918039811199094	27.421495766737465	18.914302532578557
39	18.89904148907536	34.028353677198744	28.502346595068335	18.570258238657562
40	20.691291729939902	31.146615863603166	27.481030606777413	20.68106179967952
41	15.620935803833541	30.953337263191788	29.704279548776224	23.721447384198445
42	18.22034431261996	31.720330477058546	27.815599637424437	22.24372557289706
43	17.976083764681416	31.42349479573261	28.197461132553904	22.40296030703207
44	17.365893580215648	33.73629755345348	28.83389695776967	20.063911908561195
45	17.011787059809038	36.326482354628084	26.053284520354623	20.60844606520826
46	19.906689619723306	34.73488968026437	26.740953848759702	18.61746685125262
47	18.07905388238431	32.70190067073125	28.421932635070718	20.797112811813726
48	18.748946610664785	30.91946110069019	30.283360683023936	20.048231605621094
49	19.265306533152103	29.97948144315806	30.15221633116129	20.602995692528545
50	17.330759639403343	32.15619258766086	30.67947699900803	19.833570773927764
51	16.09444741186957	34.244272287202776	27.652591568357316	22.008688732570334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2628.0
1	2599.5
2	2571.0
3	21106.5
4	39642.0
5	26066.5
6	12491.0
7	13260.5
8	14030.0
9	16431.0
10	18832.0
11	20955.0
12	23078.0
13	22760.0
14	22442.0
15	20702.0
16	18962.0
17	16884.0
18	14806.0
19	13504.5
20	12203.0
21	11741.0
22	11279.0
23	11903.5
24	12528.0
25	12167.5
26	13080.0
27	14353.0
28	16125.5
29	17898.0
30	18435.0
31	18972.0
32	22499.5
33	26027.0
34	31379.5
35	36732.0
36	37656.0
37	38580.0
38	43549.0
39	48518.0
40	58598.0
41	68678.0
42	77374.5
43	86071.0
44	91496.0
45	96921.0
46	99006.0
47	101091.0
48	97319.0
49	93547.0
50	87097.0
51	80647.0
52	70783.0
53	60919.0
54	53792.0
55	46665.0
56	42918.0
57	39171.0
58	36893.0
59	34615.0
60	30624.0
61	26633.0
62	22217.0
63	17801.0
64	14330.0
65	10859.0
66	7904.0
67	4949.0
68	3638.5
69	2328.0
70	1872.0
71	1416.0
72	1062.5
73	709.0
74	622.5
75	411.5
76	287.0
77	206.0
78	125.0
79	121.5
80	118.0
81	85.0
82	52.0
83	38.0
84	24.0
85	19.0
86	14.0
87	13.5
88	13.0
89	9.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1192579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.177981187389125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98598445987098	23.836375530973967
2	7.783557414098073	4.697840984272296
3	3.1989230767440273	2.8961112128965856
4	1.8556141180505685	2.239947517823349
5	1.2456296282419803	1.8795293743770498
6	0.8873225160183998	1.6066561317330035
7	0.69266269124918	1.463221316600685
8	0.48562292764671955	1.1724095659750027
9	0.40028190325060414	1.0871729771354142
>10	3.3511247075175237	21.56863396508816
>50	0.6438534759167699	13.884540821641306
>100	0.46551587382129894	20.278022160449602
>500	0.0030699488080966767	0.6192045376687496
>1k	5.581725105630322E-4	0.300927341279984
>5k	0.0	0.0
>10k+	2.790862552815161E-4	2.4694065620848393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29320	2.4585373379876723	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2243	0.18807978339380452	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1330	0.11152301021567544	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07152565993531665	0.0
2	0.0	0.0	0.0	0.2505494394920588	0.0
3	0.0	0.0	0.0	0.36928371202243204	0.0
4	0.0	0.0	0.0	0.5743854285544187	0.0
5	0.0	0.0	0.0	1.0979566133564318	0.0
6	0.0	0.0	0.0	1.7901539436800413	0.0
7	0.0	0.0	0.0	2.2198948665036027	0.0
8	0.0	0.0	0.0	2.9211482006642746	0.0
9	0.0	0.0	0.0	3.211946546098833	0.0
10	0.0	0.0	0.0	3.6587094020605764	0.0
11	0.0	0.0	0.0	4.206094522878568	0.0
12	0.0	0.0	0.0	4.616549511604682	0.0
13	0.0	0.0	0.0	4.796747217584747	0.0
14	0.0	0.0	0.0	4.878251252118308	0.0
15	0.0	0.0	0.0	4.973506996182223	0.0
16	0.0	0.0	0.0	5.189509458073637	0.0
17	0.0	0.0	0.0	5.443245269286144	0.0
18	0.0	0.0	0.0	5.7531618450433895	0.0
19	0.0	0.0	0.0	5.931095550064189	0.0
20	0.0	0.0	0.0	6.12194244574154	0.0
21	0.0	0.0	0.0	6.401672342041911	0.0
22	0.0	0.0	0.0	6.670585344870235	0.0
23	0.0	0.0	0.0	6.985868441419814	0.0
24	0.0	0.0	0.0	7.212268537346373	0.0
25	0.0	0.0	0.0	7.43380522380488	0.0
26	0.0	0.0	0.0	7.635804420503799	0.0
27	0.0	0.0	0.0	7.835623468130833	0.0
28	0.0	0.0	0.0	8.054225338531033	0.0
29	0.0	0.0	0.0	8.284818028826601	0.0
30	0.0	0.0	0.0	8.526479168256358	0.0
31	0.0	0.0	0.0	8.78214357287861	0.0
32	0.0	0.0	0.0	9.013323226385841	0.0
33	0.0	0.0	0.0	9.24332895346975	0.0
34	0.0	0.0	0.0	9.468638974860365	0.0
35	0.0	0.0	0.0	9.72153626719907	0.0
36	0.0	0.0	0.0	9.961017257556941	0.0
37	0.0	0.0	0.0	10.224982999029834	0.0
38	0.0	0.0	0.0	10.499262522650492	0.0
39	0.0	0.0	0.0	10.767923969816675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCAAT	35	1.2119744E-7	45.000004	19
TCGTTTC	20	7.0333935E-4	45.0	18
AATCCGA	20	7.0333935E-4	45.0	37
ATTAGCG	50	2.1827873E-11	45.0	1
TTTACGG	85	0.0	45.0	2
CGACGCG	20	7.0333935E-4	45.0	38
CGACGAT	20	7.0333935E-4	45.0	35
ACCGCGT	20	7.0333935E-4	45.0	15
GGCGTAA	40	6.8157533E-9	45.0	8
TAAGTCG	20	7.0333935E-4	45.0	1
ATAGACG	20	7.0333935E-4	45.0	1
ACTTACG	45	3.8562575E-10	45.0	1
CTACGCT	20	7.0333935E-4	45.0	33
TTGCGAA	25	3.890894E-5	45.0	1
GAACGCG	20	7.0333935E-4	45.0	1
TCGTAAG	40	6.8157533E-9	45.0	1
CGTAAGC	25	3.890894E-5	45.0	20
TATAGCG	85	0.0	45.0	1
CCGAATC	20	7.0333935E-4	45.0	19
TCGACTC	20	7.0333935E-4	45.0	38
>>END_MODULE