FastQCFastQC Report
Thu 26 May 2016
SRR1547335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547335_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences113176
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19691.739768148724111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGGG253.8684746E-545.0000043
GGATGTG302.1481337E-645.0000048
CGACGGT207.0063246E-445.00000428
CCGATAT207.0063246E-445.00000411
TCGGGGT207.0063246E-445.0000045
GGATCCA253.8684746E-545.0000048
AGGGTCT253.8684746E-545.0000046
CACGACG207.0063246E-445.00000426
TAACGGG406.721166E-945.0000043
CGACAGG207.0063246E-445.0000042
TGACGGG207.0063246E-445.0000043
GATGGGC253.8684746E-545.0000044
CGAGGGT207.0063246E-445.0000044
CGGGCGA207.0063246E-445.0000046
CGGGACA253.8684746E-545.0000046
CGGGAAC253.8684746E-545.0000046
GCGATGA207.0063246E-445.0000049
ACTAACC253.8684746E-545.00000414
CGGTCTA207.0063246E-445.00000431
GGCGATC207.0063246E-445.0000048