##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547335_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	113176
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.340080935887467	31.0	31.0	33.0	30.0	34.0
2	31.746615890294763	31.0	31.0	34.0	30.0	34.0
3	31.767406517282815	31.0	31.0	34.0	30.0	34.0
4	35.59828938997667	37.0	35.0	37.0	33.0	37.0
5	35.49788824485757	37.0	35.0	37.0	33.0	37.0
6	35.542031879550436	37.0	35.0	37.0	33.0	37.0
7	35.951694705591294	37.0	35.0	37.0	35.0	37.0
8	35.95380646073372	37.0	35.0	37.0	35.0	37.0
9	37.67802360924578	39.0	37.0	39.0	35.0	39.0
10	37.06166501731816	39.0	37.0	39.0	33.0	39.0
11	36.72351028486605	39.0	37.0	39.0	32.0	39.0
12	36.56447480031102	39.0	35.0	39.0	32.0	39.0
13	36.49390330105323	39.0	35.0	39.0	32.0	39.0
14	37.61041210150562	40.0	36.0	41.0	32.0	41.0
15	37.72636424683679	40.0	36.0	41.0	32.0	41.0
16	37.75228847105393	40.0	36.0	41.0	32.0	41.0
17	37.67376475577861	40.0	36.0	41.0	32.0	41.0
18	37.49134092033647	39.0	36.0	41.0	32.0	41.0
19	37.38309358874673	39.0	36.0	41.0	32.0	41.0
20	37.1777673711741	39.0	35.0	41.0	32.0	41.0
21	37.069555382766666	39.0	35.0	41.0	32.0	41.0
22	37.01077083480597	39.0	35.0	41.0	32.0	41.0
23	36.936302749699585	39.0	35.0	41.0	31.0	41.0
24	36.81470453099597	38.0	35.0	40.0	31.0	41.0
25	36.731170919629605	38.0	35.0	40.0	31.0	41.0
26	36.60854774863929	38.0	35.0	40.0	31.0	41.0
27	36.523087933837566	38.0	35.0	40.0	31.0	41.0
28	36.539010037463775	38.0	35.0	40.0	31.0	41.0
29	36.52894606630381	38.0	35.0	40.0	31.0	41.0
30	36.45914328126104	38.0	35.0	40.0	30.0	41.0
31	36.309526754789005	38.0	35.0	40.0	30.0	41.0
32	36.124681911359296	38.0	35.0	41.0	30.0	41.0
33	35.814377606559695	38.0	35.0	40.0	28.0	41.0
34	35.498162154520394	38.0	35.0	40.0	26.0	41.0
35	35.28204743055065	38.0	34.0	40.0	25.0	41.0
36	35.10227433378101	38.0	34.0	40.0	24.0	41.0
37	35.00838517000071	38.0	34.0	40.0	24.0	41.0
38	34.93146956952004	38.0	34.0	40.0	23.0	41.0
39	34.903601470276385	38.0	34.0	40.0	23.0	41.0
40	34.73330034636319	38.0	34.0	40.0	23.0	41.0
41	34.64015338941118	38.0	34.0	40.0	22.0	41.0
42	34.560975825263306	38.0	34.0	40.0	22.0	41.0
43	34.50329575174949	38.0	33.0	40.0	22.0	41.0
44	34.41084682264791	38.0	33.0	40.0	22.0	41.0
45	34.31025129002615	38.0	33.0	40.0	22.0	41.0
46	34.26384569166608	38.0	33.0	40.0	22.0	41.0
47	34.143042694564215	37.0	33.0	40.0	21.0	41.0
48	34.09419841662543	37.0	33.0	40.0	21.0	41.0
49	33.986693291864	37.0	33.0	40.0	20.0	41.0
50	33.940110977592425	37.0	33.0	40.0	20.0	41.0
51	33.51140701208737	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	3.0
16	9.0
17	26.0
18	46.0
19	99.0
20	180.0
21	265.0
22	404.0
23	664.0
24	970.0
25	1513.0
26	2138.0
27	2526.0
28	2660.0
29	2635.0
30	2876.0
31	3391.0
32	4152.0
33	5231.0
34	7357.0
35	9787.0
36	9391.0
37	11411.0
38	16557.0
39	28874.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.385205343889165	23.527956457199405	28.548455502933486	18.538382695977944
2	34.162720011309816	24.52109987983318	28.791439881246905	12.524740227610092
3	26.243196437407224	24.9478688061073	36.143705379232344	12.665229377253128
4	24.300204990457342	26.61871774934615	35.72842298720577	13.35265427299074
5	21.87654626422563	30.090301830776845	34.56651586908885	13.466636035908675
6	20.70757050964869	39.19912348907896	30.224605923517355	9.868700077755001
7	85.43242383544214	2.678129638792677	10.648900827030467	1.240545698734714
8	86.6835724888669	2.031349402700219	10.220364741641337	1.0647133667915458
9	81.94316816286138	5.347423482010321	10.928995546759031	1.7804128083692656
10	36.33014066586556	40.09330600127235	15.725948964444758	7.850604368417333
11	22.60991729695342	25.621156428924863	35.103732240050896	16.665194034070826
12	24.738460450979005	22.867038948186895	34.64780518837916	17.746695412454937
13	22.23881388280201	24.97084187460239	34.681381211564286	18.108963031031315
14	17.01067364105464	29.693574609457833	34.882837350675054	18.412914398812468
15	16.592740510355554	29.11924789708065	38.028380575387004	16.259631017176787
16	20.816250795221602	27.808899413303173	36.482999929313635	14.891849862161589
17	20.51053226832544	26.87672298013713	33.44083551282957	19.171909238707855
18	20.55029334841309	28.209160952852198	33.96391461087156	17.27663108786315
19	22.172545415989255	28.762281755849294	32.29483282674772	16.77034000141373
20	23.335336113663672	29.10245988548809	31.906057821446243	15.656146179401995
21	23.451085035696615	26.095638651304164	34.73174524634198	15.72153106665724
22	21.89245069626069	24.74552908743903	34.610694846964016	18.751325369336254
23	18.51894394571287	27.608326853749908	35.274263094649044	18.59846610588817
24	18.01795433660847	28.202975895949674	36.839082490987494	16.93998727645437
25	19.511203788789143	29.64232699512264	33.47971301335973	17.366756202728496
26	19.096804976320065	31.55969463490493	31.457199406234537	17.88630098254047
27	17.930479960415635	30.91821587615749	34.48611012935604	16.665194034070826
28	16.512334770622747	30.55064678023609	35.766416908178414	17.17060154096275
29	17.595603308121863	28.376157489220326	35.70368275959567	18.324556443062136
30	19.008447020569733	28.261292146744893	35.199158832261254	17.531102000424116
31	20.778256874248957	29.088322612568035	32.43708913550576	17.696331377677247
32	21.298685233618436	29.052095850710398	32.34961475931293	17.29960415635824
33	20.64218562239344	30.07704813741429	31.591503498975047	17.689262741217217
34	18.949247190217008	30.165406093164627	32.54046794373365	18.34487877288471
35	19.019049975259772	30.187495582102216	32.00325157277161	18.790202869866402
36	21.27836290379586	29.999293136353998	30.56301689404114	18.159327065809006
37	19.69410475719234	31.17357036827596	31.15236445889588	17.979960415635823
38	19.579239414716902	31.24160599420372	30.337704106877784	18.841450484201598
39	19.68173464338729	30.486145472538347	29.77574750830565	20.056372375768714
40	20.50346363186541	29.669717961405244	30.97918286562522	18.847635541104122
41	18.555170707570507	29.70771188237789	31.345868381989117	20.391249028062486
42	19.69675549586485	30.359793595815365	30.045239273344173	19.898211634975613
43	20.03958436417615	30.23255813953488	29.697992507245353	20.029864989043613
44	19.38043401427865	31.474870997384603	30.520605075280976	18.624089913055773
45	19.369831059588606	30.75828797624938	29.496536368134585	20.375344596027425
46	19.648158620202164	30.96592917226267	29.94274404467378	19.443168162861387
47	19.53152611861172	30.37128013006291	30.675231497844063	19.421962253481304
48	19.831943168162862	29.7898847812257	30.995970877217783	19.38220117339365
49	19.478511345161518	30.014313988831553	30.695553827666643	19.811620838340286
50	18.566657241818053	30.772425249169434	31.253976108008764	19.406941401003746
51	18.462394854032656	31.033081218632923	30.817487806602106	19.68703612073231
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	105.0
1	145.0
2	185.0
3	1577.5
4	2970.0
5	1964.5
6	959.0
7	1010.5
8	1062.0
9	1117.5
10	1173.0
11	1224.5
12	1276.0
13	1238.5
14	1201.0
15	1201.5
16	1202.0
17	1112.5
18	1023.0
19	961.0
20	899.0
21	906.0
22	913.0
23	1000.5
24	1088.0
25	1242.5
26	1546.0
27	1695.0
28	2036.5
29	2378.0
30	2847.0
31	3316.0
32	3632.0
33	3948.0
34	4471.5
35	4995.0
36	5292.5
37	5590.0
38	5896.0
39	6202.0
40	6783.0
41	7364.0
42	7498.0
43	7632.0
44	7809.0
45	7986.0
46	7834.5
47	7683.0
48	7825.5
49	7968.0
50	7516.5
51	7065.0
52	6471.5
53	5878.0
54	5294.0
55	4710.0
56	4342.0
57	3974.0
58	3560.0
59	3146.0
60	2740.0
61	2334.0
62	1990.0
63	1646.0
64	1329.5
65	1013.0
66	742.5
67	472.0
68	406.5
69	341.0
70	260.0
71	179.0
72	139.5
73	100.0
74	73.5
75	36.5
76	26.0
77	20.5
78	15.0
79	13.5
80	12.0
81	9.5
82	7.0
83	4.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	113176.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.84558563653071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.61760361456251	34.88902240757758
2	11.578764445216787	11.774581183289744
3	6.579198887826918	10.035696614123136
4	4.377443739681988	8.90294762140383
5	2.867321226865931	7.289531349402701
6	1.9289251889825352	5.884639852972361
7	1.3033278303936051	4.638792676892627
8	0.9158050221565732	3.725171414434156
9	0.6394995221131289	2.92641549445112
>10	1.1747328177947693	7.6853749911642035
>50	0.01563993396472326	0.5080582455644306
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0017377704405248065	1.739768148724111
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1969	1.739768148724111	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014137272920053722	0.0
2	0.0	0.0	0.0	0.052131193892698095	0.0
3	0.0	0.0	0.0	0.10249522867038949	0.0
4	0.0	0.0	0.0	0.16611295681063123	0.0
5	0.0	0.0	0.0	0.29511557220612145	0.0
6	0.0	0.0	0.0	0.5593058598996253	0.0
7	0.0	0.0	0.0	0.7430904078603238	0.0
8	0.0	0.0	0.0	0.9604509790061497	0.0
9	0.0	0.0	0.0	1.0850356966141232	0.0
10	0.0	0.0	0.0	1.2175726302396268	0.0
11	0.0	0.0	0.0	1.3598289389976674	0.0
12	0.0	0.0	0.0	1.4720435428005938	0.0
13	0.0	0.0	0.0	1.5436134869583658	0.0
14	0.0	0.0	0.0	1.5975118399660706	0.0
15	0.0	0.0	0.0	1.6399236587262318	0.0
16	0.0	0.0	0.0	1.7079592846539902	0.0
17	0.0	0.0	0.0	1.768926274121722	0.0
18	0.0	0.0	0.0	1.8484484342970242	0.0
19	0.0	0.0	0.0	1.914716901109776	0.0
20	0.0	0.0	0.0	1.9915883226125681	0.0
21	0.0	0.0	0.0	2.0596239485403265	0.0
22	0.0	0.0	0.0	2.1515162225206756	0.0
23	0.0	0.0	0.0	2.244292076058528	0.0
24	0.0	0.0	0.0	2.324697815791334	0.0
25	0.0	0.0	0.0	2.3953841803916025	0.0
26	0.0	0.0	0.0	2.480207817911925	0.0
27	0.0	0.0	0.0	2.5508941825121934	0.0
28	0.0	0.0	0.0	2.6206969675549585	0.0
29	0.0	0.0	0.0	2.717890718880328	0.0
30	0.0	0.0	0.0	2.8159680497632005	0.0
31	0.0	0.0	0.0	2.914928960203577	0.0
32	0.0	0.0	0.0	3.021842086661483	0.0
33	0.0	0.0	0.0	3.1172686788718456	0.0
34	0.0	0.0	0.0	3.2206474870997384	0.0
35	0.0	0.0	0.0	3.346115784265215	0.0
36	0.0	0.0	0.0	3.4574468085106385	0.0
37	0.0	0.0	0.0	3.572312150986075	0.0
38	0.0	0.0	0.0	3.7074998232840883	0.0
39	0.0	0.0	0.0	3.891284371244787	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGGG	25	3.8684746E-5	45.000004	3
GGATGTG	30	2.1481337E-6	45.000004	8
CGACGGT	20	7.0063246E-4	45.000004	28
CCGATAT	20	7.0063246E-4	45.000004	11
TCGGGGT	20	7.0063246E-4	45.000004	5
GGATCCA	25	3.8684746E-5	45.000004	8
AGGGTCT	25	3.8684746E-5	45.000004	6
CACGACG	20	7.0063246E-4	45.000004	26
TAACGGG	40	6.721166E-9	45.000004	3
CGACAGG	20	7.0063246E-4	45.000004	2
TGACGGG	20	7.0063246E-4	45.000004	3
GATGGGC	25	3.8684746E-5	45.000004	4
CGAGGGT	20	7.0063246E-4	45.000004	4
CGGGCGA	20	7.0063246E-4	45.000004	6
CGGGACA	25	3.8684746E-5	45.000004	6
CGGGAAC	25	3.8684746E-5	45.000004	6
GCGATGA	20	7.0063246E-4	45.000004	9
ACTAACC	25	3.8684746E-5	45.000004	14
CGGTCTA	20	7.0063246E-4	45.000004	31
GGCGATC	20	7.0063246E-4	45.000004	8
>>END_MODULE