FastQCFastQC Report
Thu 26 May 2016
SRR1547334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547334_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences731086
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55290.7562721759136408No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG10880.14881970110219592No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG8170.11175155864015997No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT207.0316E-445.08
CGGTTTA207.0316E-445.034
CAATTCG207.0316E-445.01
ACGGGTA351.2111195E-745.05
TACGACG207.0316E-445.01
TATAGCG453.8380676E-1045.01
ATAACGG406.8084773E-945.02
GCGTATA207.0316E-445.030
TCGAATC253.8894075E-545.013
GATCGCT302.1644137E-644.9999969
GGCACGC302.1644137E-644.9999969
TAGGTAT302.1644137E-644.99999617
CGGTCTA1700.043.67646831
CGTTTTT25000.042.2100031
GTAACGG800.042.18752
ATAGCGG1350.041.6666682
AGGGCGC1300.041.538466
TGGGCGA3200.041.4843756
CGACGGT1850.041.35135328
CGGGGTA1150.041.086966
Produced by FastQC (version 0.11.5)