##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547334_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	731086
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289442008190555	31.0	31.0	33.0	30.0	34.0
2	31.702158159231608	31.0	31.0	34.0	30.0	34.0
3	31.846283474174037	31.0	31.0	34.0	30.0	34.0
4	35.603554164626324	37.0	35.0	37.0	33.0	37.0
5	35.471993718933206	37.0	35.0	37.0	33.0	37.0
6	35.510347619842264	37.0	35.0	37.0	33.0	37.0
7	35.90058488331058	37.0	35.0	37.0	35.0	37.0
8	35.8745181825394	37.0	35.0	37.0	35.0	37.0
9	37.548303482764	39.0	37.0	39.0	35.0	39.0
10	36.91418930194259	39.0	37.0	39.0	32.0	39.0
11	36.619385407462325	39.0	35.0	39.0	32.0	39.0
12	36.13401296153941	38.0	35.0	39.0	32.0	39.0
13	35.917952197142334	38.0	35.0	39.0	31.0	39.0
14	37.00424710635958	39.0	35.0	41.0	31.0	41.0
15	37.15726193635222	39.0	35.0	41.0	32.0	41.0
16	37.21730822365632	39.0	35.0	41.0	32.0	41.0
17	37.08327748035115	39.0	35.0	41.0	32.0	41.0
18	37.008714433048915	39.0	35.0	41.0	31.0	41.0
19	36.98391297330273	39.0	35.0	41.0	31.0	41.0
20	36.85597453651144	38.0	35.0	41.0	31.0	41.0
21	36.72712786183842	38.0	35.0	41.0	31.0	41.0
22	36.63858287533888	38.0	35.0	40.0	31.0	41.0
23	36.58064167553475	38.0	35.0	40.0	31.0	41.0
24	36.44482181302884	38.0	35.0	40.0	31.0	41.0
25	36.38258973636481	38.0	35.0	40.0	30.0	41.0
26	36.21833135910139	38.0	35.0	40.0	30.0	41.0
27	36.16290422740963	38.0	35.0	40.0	30.0	41.0
28	36.23872157311178	38.0	35.0	40.0	30.0	41.0
29	36.28995084025682	38.0	35.0	40.0	30.0	41.0
30	36.24868072976367	38.0	35.0	40.0	30.0	41.0
31	36.149536443045	38.0	35.0	40.0	30.0	41.0
32	36.03398505784545	38.0	35.0	40.0	30.0	41.0
33	35.87817849062901	38.0	35.0	40.0	29.0	41.0
34	35.669692758444285	38.0	34.0	40.0	28.0	41.0
35	35.58772702527473	38.0	34.0	40.0	27.0	41.0
36	35.47311123451961	38.0	34.0	40.0	27.0	41.0
37	35.38618849218833	38.0	34.0	40.0	27.0	41.0
38	35.310919645568376	38.0	34.0	40.0	27.0	41.0
39	35.29119829951606	38.0	34.0	40.0	27.0	41.0
40	35.144884459557424	38.0	34.0	40.0	26.0	41.0
41	35.095564680489026	38.0	34.0	40.0	26.0	41.0
42	35.016854378281074	38.0	34.0	40.0	26.0	41.0
43	34.961692605247535	37.0	34.0	40.0	26.0	41.0
44	34.83592627953483	37.0	34.0	40.0	25.0	41.0
45	34.731960398639835	37.0	33.0	40.0	24.0	41.0
46	34.659758222698834	37.0	33.0	40.0	24.0	41.0
47	34.52777238245569	37.0	33.0	40.0	24.0	41.0
48	34.48514948993689	37.0	33.0	40.0	24.0	41.0
49	34.38639776989301	36.0	33.0	40.0	24.0	41.0
50	34.31798447788632	36.0	33.0	40.0	24.0	41.0
51	33.897623535398026	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	5.0
14	11.0
15	27.0
16	77.0
17	167.0
18	430.0
19	701.0
20	1208.0
21	1832.0
22	2735.0
23	4080.0
24	5588.0
25	7755.0
26	10383.0
27	12957.0
28	14950.0
29	16938.0
30	19929.0
31	23822.0
32	29357.0
33	37148.0
34	58940.0
35	78103.0
36	56541.0
37	71240.0
38	104244.0
39	171867.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.02607353991186	23.23064591580197	29.147596862749392	14.595683681536784
2	30.45674517088277	26.53189911993938	30.079224605586756	12.932131103591097
3	29.433746508618686	25.667158172909893	31.374968197995855	13.524127120475566
4	27.037585181497114	28.390230424327644	30.755615618408775	13.816568775766463
5	23.449361634609332	32.125632278555464	29.621002180318044	14.804003906517154
6	21.451238294810736	42.09381659613233	25.92403629668739	10.530908812369544
7	88.45635123637985	3.818565804843753	6.1580990471709205	1.5669839116054747
8	88.86724680817305	3.386195331329009	6.164527839406034	1.5820300210919096
9	83.39196756605925	6.617278952134223	7.5151213400338674	2.4756321417726506
10	40.394426921046225	37.43937101791034	12.735437417759332	9.430764643284101
11	34.63792221434961	22.416241044145284	25.82651015065259	17.119326590852513
12	32.68124406704546	21.74326960166109	28.455475826373366	17.120010504920078
13	24.3432373209171	27.18667844822634	28.71987700489409	19.75020722596247
14	18.581261301679966	32.45746738413812	29.616351564658604	19.344919749523314
15	19.537920299390223	27.974821019688516	36.43948865112996	16.047770029791298
16	23.7561654853191	25.081727731074043	34.67923062403055	16.4828761595763
17	21.655044686945175	25.757024481387962	28.934762804923086	23.653168026743778
18	23.137907168240123	27.929272342788675	30.508722640017726	18.424097848953476
19	26.327545596550884	28.665027096675356	26.585791548463515	18.421635758310238
20	29.005069171068794	27.541493066479184	26.25559783664302	17.197839925809003
21	26.036608552208634	27.161100062099397	28.829576821331553	17.972714564360416
22	23.845621445356635	24.674114946805165	30.457155519323308	21.023108088514896
23	22.22748076149728	27.866625814199697	29.60239971768027	20.30349370662275
24	22.737817438714462	26.75047805593323	32.52367026587844	17.98803423947388
25	21.873623622939025	26.939375121394747	30.51515143225284	20.67184982341339
26	19.312365439907207	31.765072782135068	28.208172499541778	20.714389278415947
27	20.04182818437229	30.527051537028477	29.174406294197947	20.256713984401287
28	18.09923866685999	30.552082791901363	32.588642102297136	18.76003643894152
29	20.472146915684338	26.763882771657503	32.79149101473698	19.972479297921176
30	21.061407276298546	28.483242737516516	30.984863613856646	19.47048637232829
31	26.507004647880006	27.270663095723346	27.401837813882363	18.82049444251429
32	27.353963829152793	27.62164779519783	27.4242701952985	17.600118180350876
33	24.66412980141871	27.979608418161472	27.530687224211654	19.82557455620816
34	20.540538322440863	28.391324686835752	30.203423400256607	20.864713590466785
35	20.671576257786363	27.10925937577795	30.85847629417059	21.360688072265095
36	24.691349581307808	29.348120467359518	27.50825484279551	18.452275108537165
37	22.166202061043432	31.25979706901787	27.72327742563802	18.85072344430067
38	21.784441228528518	32.40179677903831	25.714758592012434	20.099003400420745
39	22.23691877562968	29.701293691850207	27.23222712512618	20.82956040739393
40	22.57490910782042	28.087256492396246	27.40033320293372	21.937501196849617
41	19.01445247207579	28.38831546493846	29.391070270802615	23.206161792183135
42	21.032546102647295	28.236349759125467	26.65213121301735	24.078972925209893
43	20.68443384225659	26.73310663861707	28.063456282844975	24.519003236281367
44	20.16930976656645	28.735470245634577	28.750926703561554	22.34429328423742
45	21.8398382680013	29.9717133141655	26.42698670197487	21.761461715858328
46	22.13638340769759	30.389448026634348	27.344936163460932	20.12923240220713
47	21.13472286434154	27.426732285941736	28.883058901415154	22.555485948301566
48	20.822584483904766	26.349157281085944	31.493011766057617	21.33524646895167
49	21.492136356051134	25.93716744678465	31.18757574348298	21.383120453681236
50	19.493055536557943	28.99453689442829	30.02136547547074	21.49104209354303
51	19.347518622980058	29.385598958262094	29.257296679186855	22.009585739570994
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	977.0
1	1364.5
2	1752.0
3	5371.0
4	8990.0
5	6117.0
6	3244.0
7	3223.5
8	3203.0
9	3363.5
10	3524.0
11	3507.0
12	3490.0
13	3449.5
14	3409.0
15	3314.5
16	3220.0
17	2886.5
18	2553.0
19	2591.0
20	2629.0
21	3041.5
22	3454.0
23	3814.0
24	4174.0
25	5582.5
26	8329.5
27	9668.0
28	11258.0
29	12848.0
30	15854.0
31	18860.0
32	18830.5
33	18801.0
34	21601.0
35	24401.0
36	25661.0
37	26921.0
38	29854.0
39	32787.0
40	39003.0
41	45219.0
42	50020.5
43	54822.0
44	57330.5
45	59839.0
46	58944.0
47	58049.0
48	54831.0
49	51613.0
50	47694.0
51	43775.0
52	42012.5
53	40250.0
54	37541.0
55	34832.0
56	33378.5
57	31925.0
58	31422.5
59	30920.0
60	30017.5
61	29115.0
62	25027.0
63	20939.0
64	17251.0
65	13563.0
66	10770.5
67	7978.0
68	6450.5
69	4923.0
70	3925.0
71	2927.0
72	2400.0
73	1873.0
74	1493.5
75	877.0
76	640.0
77	523.0
78	406.0
79	291.0
80	176.0
81	169.5
82	163.0
83	115.5
84	68.0
85	45.5
86	23.0
87	22.0
88	21.0
89	15.5
90	10.0
91	7.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	731086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.955676750263297
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3634536893258	17.644340117375915
2	5.653166917303804	2.482382109032095
3	1.8064652132456418	1.1898649884785026
4	1.0103740005045103	0.8873377980778958
5	0.7038373059474929	0.7726612187079662
6	0.5277146893884976	0.6951799881947672
7	0.4437258870341832	0.6819611499012462
8	0.39208823787162445	0.6886850086631784
9	0.3404168003553679	0.6726673106065236
>10	6.477038806222311	36.12893844273292
>50	2.070992467984687	30.837529316462586
>100	0.20634888720861916	5.974807343367192
>500	0.003126498291039684	0.43520540621493564
>1k	6.252996582079368E-4	0.1493701835841798
>5k	6.252996582079368E-4	0.7590696186001196
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5529	0.7562721759136408	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1088	0.14881970110219592	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	817	0.11175155864015997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.367828135130477E-4	0.0	0.0	0.024073775178296397	0.0
2	1.367828135130477E-4	0.0	0.0	0.08740421783483748	0.0
3	1.367828135130477E-4	0.0	0.0	0.12310453216174294	0.0
4	1.367828135130477E-4	0.0	0.0	0.22569164229652872	0.0
5	1.367828135130477E-4	0.0	0.0	0.4025518201688994	0.0
6	1.367828135130477E-4	0.0	0.0	0.622225018670854	0.0
7	2.735656270260954E-4	0.0	0.0	0.7349340570056053	0.0
8	2.735656270260954E-4	0.0	0.0	0.9911282667155438	0.0
9	2.735656270260954E-4	0.0	0.0	1.0647174203855634	0.0
10	2.735656270260954E-4	0.0	0.0	1.2166831261985593	0.0
11	2.735656270260954E-4	0.0	0.0	1.4085894135573653	0.0
12	2.735656270260954E-4	0.0	0.0	1.5773794054324661	0.0
13	2.735656270260954E-4	0.0	0.0	1.6357856668025377	0.0
14	2.735656270260954E-4	0.0	0.0	1.6593123107267818	0.0
15	2.735656270260954E-4	0.0	0.0	1.6966540188158439	0.0
16	2.735656270260954E-4	0.0	0.0	1.7783133584831332	0.0
17	4.1034844053914314E-4	0.0	0.0	1.8837729077016931	0.0
18	4.1034844053914314E-4	0.0	0.0	2.015494757114758	0.0
19	4.1034844053914314E-4	0.0	0.0	2.088947127971265	0.0
20	4.1034844053914314E-4	0.0	0.0	2.1774456083142066	0.0
21	4.1034844053914314E-4	0.0	0.0	2.2842729856678967	0.0
22	4.1034844053914314E-4	0.0	0.0	2.4065568209485613	0.0
23	4.1034844053914314E-4	0.0	0.0	2.5333544890751565	0.0
24	4.1034844053914314E-4	0.0	0.0	2.6251357569424116	0.0
25	4.1034844053914314E-4	0.0	0.0	2.706247965355649	0.0
26	4.1034844053914314E-4	0.0	0.0	2.783256689363495	0.0
27	4.1034844053914314E-4	0.0	0.0	2.8632746352686276	0.0
28	4.1034844053914314E-4	0.0	0.0	2.955603034389935	0.0
29	4.1034844053914314E-4	0.0	0.0	3.0554544882544596	0.0
30	4.1034844053914314E-4	0.0	0.0	3.1695313547243416	0.0
31	4.1034844053914314E-4	0.0	0.0	3.2844289180753017	0.0
32	4.1034844053914314E-4	0.0	0.0	3.388520639158731	0.0
33	4.1034844053914314E-4	0.0	0.0	3.497262975901604	0.0
34	4.1034844053914314E-4	0.0	0.0	3.610108797049868	0.0
35	4.1034844053914314E-4	0.0	0.0	3.729793758873785	0.0
36	4.1034844053914314E-4	0.0	0.0	3.853171856662554	0.0
37	4.1034844053914314E-4	0.0	0.0	3.9844833576350798	0.0
38	4.1034844053914314E-4	0.0	0.0	4.117025903929223	0.0
39	4.1034844053914314E-4	0.0	0.0	4.261605337812514	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	20	7.0316E-4	45.0	8
CGGTTTA	20	7.0316E-4	45.0	34
CAATTCG	20	7.0316E-4	45.0	1
ACGGGTA	35	1.2111195E-7	45.0	5
TACGACG	20	7.0316E-4	45.0	1
TATAGCG	45	3.8380676E-10	45.0	1
ATAACGG	40	6.8084773E-9	45.0	2
GCGTATA	20	7.0316E-4	45.0	30
TCGAATC	25	3.8894075E-5	45.0	13
GATCGCT	30	2.1644137E-6	44.999996	9
GGCACGC	30	2.1644137E-6	44.999996	9
TAGGTAT	30	2.1644137E-6	44.999996	17
CGGTCTA	170	0.0	43.676468	31
CGTTTTT	2500	0.0	42.210003	1
GTAACGG	80	0.0	42.1875	2
ATAGCGG	135	0.0	41.666668	2
AGGGCGC	130	0.0	41.53846	6
TGGGCGA	320	0.0	41.484375	6
CGACGGT	185	0.0	41.351353	28
CGGGGTA	115	0.0	41.08696	6
>>END_MODULE