FastQCFastQC Report
Thu 26 May 2016
SRR1547333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547333_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2121596
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT707573.3350835880158143No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT43890.2068725619769268No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT35100.16544148838892986No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC28200.1329188026372599No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT207.0346374E-445.042
CGTTTTT266850.044.173691
CGACGGT2550.042.3529428
CGAACGG650.041.5384642
GCGTAAG1150.041.0869561
ACGGTCT2700.040.83333630
GCGTTAG1000.040.51
GCGCGAC2300.040.1086929
CGGGTTC451.929584E-840.06
CGTTAGG2600.039.8076932
GTTTTTT300850.039.667612
TACGGGA8500.039.4411774
GACGGTC2750.039.27272829
TAGGGAC27900.038.8709685
TAGGGCG4250.038.647065
GCGCGAA356.250788E-638.5714334
GTTAGCG1400.038.571431
CGATTCG356.250788E-638.5714310
CACGACG2800.038.5714326
GTAGGGA35900.038.419224
Produced by FastQC (version 0.11.5)