##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547333_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2121596
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334638168623997	31.0	31.0	33.0	30.0	34.0
2	31.737151182411733	31.0	31.0	34.0	30.0	34.0
3	31.64882522402946	31.0	31.0	34.0	30.0	34.0
4	35.519556975032	37.0	35.0	37.0	33.0	37.0
5	35.44968080633636	37.0	35.0	37.0	33.0	37.0
6	35.50861049888857	37.0	35.0	37.0	33.0	37.0
7	35.96173823857134	37.0	35.0	37.0	35.0	37.0
8	35.963105134059454	37.0	35.0	37.0	35.0	37.0
9	37.666824880891554	39.0	37.0	39.0	35.0	39.0
10	37.051565896617454	39.0	37.0	39.0	33.0	39.0
11	36.72812448741419	39.0	37.0	39.0	32.0	39.0
12	36.61968301222287	39.0	35.0	39.0	32.0	39.0
13	36.56682610638406	39.0	35.0	39.0	32.0	39.0
14	37.737857254632836	40.0	36.0	41.0	32.0	41.0
15	37.83343435790791	40.0	36.0	41.0	32.0	41.0
16	37.85088254314205	40.0	36.0	41.0	33.0	41.0
17	37.75658560819308	40.0	36.0	41.0	32.0	41.0
18	37.504705891225285	39.0	36.0	41.0	32.0	41.0
19	37.319969966006724	39.0	36.0	41.0	32.0	41.0
20	37.03656445430704	39.0	35.0	41.0	32.0	41.0
21	36.925086585759026	38.0	35.0	41.0	32.0	41.0
22	36.86140716705725	38.0	35.0	41.0	31.0	41.0
23	36.77036815680271	38.0	35.0	40.0	31.0	41.0
24	36.609593909490776	38.0	35.0	40.0	31.0	41.0
25	36.5353248215023	38.0	35.0	40.0	31.0	41.0
26	36.3993470010313	38.0	35.0	40.0	31.0	41.0
27	36.30094843693144	38.0	35.0	40.0	30.0	41.0
28	36.26745478403994	38.0	35.0	40.0	30.0	41.0
29	36.199822209317894	38.0	35.0	40.0	30.0	41.0
30	36.09127138248753	38.0	35.0	40.0	30.0	41.0
31	35.910668195075786	38.0	35.0	40.0	30.0	41.0
32	35.63081142686921	38.0	35.0	40.0	28.0	41.0
33	35.22928352051946	38.0	34.0	40.0	25.0	41.0
34	34.81184636471788	38.0	34.0	40.0	23.0	41.0
35	34.51189434746295	38.0	34.0	40.0	21.0	41.0
36	34.32439870738821	38.0	33.0	40.0	21.0	41.0
37	34.21081063501251	38.0	33.0	40.0	20.0	41.0
38	34.11468583085564	38.0	33.0	40.0	18.0	41.0
39	34.06010805073162	38.0	33.0	40.0	18.0	41.0
40	33.92277229029467	38.0	33.0	40.0	18.0	41.0
41	33.81007647073241	37.0	33.0	40.0	18.0	41.0
42	33.728636837550596	37.0	33.0	40.0	18.0	41.0
43	33.673269557446375	37.0	33.0	40.0	18.0	41.0
44	33.54706739643174	37.0	33.0	40.0	17.0	41.0
45	33.46464831193121	37.0	33.0	40.0	17.0	41.0
46	33.392046365094956	37.0	33.0	40.0	17.0	41.0
47	33.27090548813252	37.0	32.0	40.0	17.0	41.0
48	33.200440611690446	37.0	32.0	40.0	16.0	41.0
49	33.082493085394205	36.0	32.0	40.0	15.0	41.0
50	33.0170748813629	36.0	32.0	40.0	15.0	41.0
51	32.637345658645664	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	4.0
13	14.0
14	25.0
15	94.0
16	222.0
17	529.0
18	1230.0
19	2461.0
20	4196.0
21	6447.0
22	9881.0
23	14633.0
24	22385.0
25	35168.0
26	50510.0
27	58429.0
28	57878.0
29	55833.0
30	58531.0
31	66412.0
32	78826.0
33	96465.0
34	134988.0
35	181960.0
36	187739.0
37	212578.0
38	295557.0
39	488460.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.31250624529835	21.986466791981133	26.717857688268644	22.983169274451875
2	38.45864151327586	23.338609235688605	26.465264828930675	11.737484422104869
3	25.754337772130036	23.291946251784033	39.090288631765894	11.86342734432003
4	22.892577097618965	25.446126406723995	38.70859485029195	12.952701645365092
5	20.709927809064496	28.886790887614794	37.782876664548766	12.620404638771943
6	19.549669211291874	37.3874667938665	33.804409510576	9.258454484265618
7	80.37307762646611	2.67030103752081	15.463735791357072	1.492885544656004
8	81.11779999585218	2.288088778447923	15.111595233022687	1.482515992677211
9	76.3652457866625	5.357758970133805	16.205394429476677	2.0716008137270245
10	32.75260699963612	38.44021199134991	20.63983906455329	8.167341944460679
11	20.494382530887126	25.561888314269073	37.84231305111812	16.101416103725686
12	22.782141369044815	22.923025872974872	37.349382257508026	16.945450500472287
13	21.16953463336092	23.7927013437054	37.910280750906395	17.127483272027284
14	15.63808566758233	28.31769102128775	37.99088045037792	18.053342860752
15	15.541554565525198	29.503025081118178	40.00394042975194	14.951479923604683
16	19.610519627676524	27.777625900501317	38.778966400766215	13.83288807105594
17	19.112404058077033	27.579567457706368	35.914236263643026	17.393792220573566
18	18.550987087079726	29.148622075079327	36.25412189691157	16.046268940929377
19	20.415856741811353	29.63566107779238	33.87897601616896	16.069506164227306
20	21.66873429248547	29.456409231540782	34.073310847116986	14.801545628856767
21	21.627161815915944	27.376748447866607	36.09410085614792	14.901988880069533
22	19.91557299316175	25.64130965556119	36.81139104711736	17.6317263041597
23	17.71812352587392	28.61666405856723	36.67705821466481	16.988154200894044
24	17.502059770097606	28.50830224038884	37.520668402466825	16.468969587046733
25	18.569935086604612	29.75594788074638	35.15688189457371	16.517235138075296
26	17.75663227117698	32.29945757816285	33.195763943748005	16.748146206912153
27	17.016104856909607	31.00557316284533	35.900048831162955	16.078273149082108
28	16.479197736043997	30.3223139560972	37.39967458460517	15.798813723253627
29	16.913069217702144	28.997462287824828	36.465472219970245	17.62399627450278
30	17.65152272157376	28.929824528326787	36.2419141061729	17.176738643926555
31	19.40656939398453	28.644614714582794	34.74356097956444	17.205254911868234
32	19.35538151467103	29.886651370006355	34.41305507740399	16.344912037918625
33	18.923772480717346	29.976913606549033	33.96103687978296	17.138277032950665
34	17.577616096561268	30.689914573745426	34.06185720561313	17.670612124080172
35	17.735327555293278	30.577404934775522	33.694492259600786	17.99277525033041
36	18.739995739056823	31.77480538236309	32.12407074673972	17.36112813184037
37	17.88200958146603	32.565248049110195	32.411165933570764	17.141576435853008
38	17.944698236610552	32.48841909581277	31.287719245322858	18.279163422253813
39	17.95860286312757	31.344091900625752	31.477010703263016	19.220294532983658
40	19.419625602612374	30.787624033981963	32.05746994243956	17.735280420966106
41	17.271997119149923	31.63231831130903	32.69213365786889	18.403550911672156
42	18.55928272866276	32.596215302065055	30.34833210469854	18.49616986457365
43	18.86688134781551	31.859222962335902	30.96635740263462	18.307538287213966
44	18.90572003340881	32.36681253169784	30.93204361245025	17.795423822443105
45	18.440457089851225	31.99958898866702	30.49991610089763	19.060037820584128
46	18.952948629239497	31.700568817060365	30.22993067483159	19.116551878868552
47	18.409112762278966	31.95311454207116	31.393064466561967	18.244708229087912
48	18.352551569667362	31.558835895241128	31.46362455434494	18.62498798074657
49	18.040522323760037	31.16663115880686	31.512738523262673	19.280107994170425
50	17.124608078069528	31.80539556070053	32.44910906694771	18.62088729428223
51	17.138889779203957	31.782676815001537	32.05285077837628	19.025582627418228
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2654.0
1	3178.0
2	3702.0
3	49939.0
4	96176.0
5	61949.5
6	27723.0
7	27994.5
8	28266.0
9	30595.0
10	32924.0
11	34632.5
12	36341.0
13	37076.5
14	37812.0
15	36027.0
16	34242.0
17	31760.0
18	29278.0
19	26762.0
20	24246.0
21	23504.5
22	22763.0
23	23332.0
24	23901.0
25	25442.5
26	29596.5
27	32209.0
28	37907.5
29	43606.0
30	50957.0
31	58308.0
32	62838.0
33	67368.0
34	73972.0
35	80576.0
36	86152.5
37	91729.0
38	99267.5
39	106806.0
40	111268.5
41	115731.0
42	122871.5
43	130012.0
44	136056.5
45	142101.0
46	146090.5
47	150080.0
48	147173.5
49	144267.0
50	132611.0
51	120955.0
52	108161.0
53	95367.0
54	86008.0
55	76649.0
56	71624.0
57	66599.0
58	61705.5
59	56812.0
60	50049.0
61	43286.0
62	37833.5
63	32381.0
64	25172.5
65	17964.0
66	13841.5
67	9719.0
68	7367.5
69	5016.0
70	4086.5
71	3157.0
72	2518.0
73	1879.0
74	1432.5
75	704.0
76	422.0
77	370.5
78	319.0
79	218.5
80	118.0
81	102.0
82	86.0
83	57.5
84	29.0
85	28.5
86	28.0
87	17.5
88	7.0
89	6.0
90	5.0
91	4.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2121596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.46429232898048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.7400802461618	20.30871916983846
2	6.73264651354686	3.5634945096439075
3	3.2118540837518785	2.5499833617131844
4	2.1424930689452513	2.267982515575267
5	1.5050254437870325	1.9914716653466804
6	1.089614119887833	1.7301519956697844
7	0.8031397008551227	1.4878166677107931
8	0.6245976088396777	1.3223626966652349
9	0.4978330748376696	1.1857320021185378
>10	5.342393534996851	32.43566628695174
>50	1.141969953736695	20.66487347559561
>100	0.16383195275423776	5.712340387050993
>500	0.002712418739460813	0.5009921974007829
>1k	0.0016274512436764879	0.892348983182004
>5k	0.0	0.0
>10k+	1.8082791596405422E-4	3.3860640855370336
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70757	3.3350835880158143	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT	4389	0.2068725619769268	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT	3510	0.16544148838892986	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	2820	0.1329188026372599	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.426865435266657E-5	0.0	0.0	0.04237376013152363	0.0
2	9.426865435266657E-5	0.0	0.0	0.1336729518720812	0.0
3	9.426865435266657E-5	0.0	0.0	0.21912748704277346	0.0
4	9.426865435266657E-5	0.0	0.0	0.35501575229214233	0.0
5	9.426865435266657E-5	0.0	0.0	0.6961740123944427	0.0
6	1.4140298152899987E-4	0.0	0.0	1.3592125927839231	0.0
7	1.4140298152899987E-4	0.0	0.0	1.8198563722782284	0.0
8	1.4140298152899987E-4	0.0	0.0	2.3243822103736997	0.0
9	1.4140298152899987E-4	0.0	0.0	2.6242507998695324	0.0
10	1.4140298152899987E-4	0.0	0.0	2.89885538999885	0.0
11	1.4140298152899987E-4	0.0	0.0	3.1424455928461406	0.0
12	1.4140298152899987E-4	0.0	0.0	3.3250439763272555	0.0
13	1.4140298152899987E-4	0.0	0.0	3.44080588387233	0.0
14	1.4140298152899987E-4	0.0	0.0	3.5193316729481015	0.0
15	1.4140298152899987E-4	0.0	0.0	3.580936238567569	0.0
16	1.4140298152899987E-4	0.0	0.0	3.6636098484348576	0.0
17	1.4140298152899987E-4	0.0	0.0	3.759009726639756	0.0
18	1.4140298152899987E-4	0.0	0.0	3.883444350385276	0.0
19	1.8853730870533315E-4	0.0	0.0	3.971114198933256	0.0
20	1.8853730870533315E-4	0.0	0.0	4.063026136927106	0.0
21	1.8853730870533315E-4	0.0	0.0	4.160547059854939	0.0
22	1.8853730870533315E-4	0.0	0.0	4.273716579405316	0.0
23	1.8853730870533315E-4	0.0	0.0	4.399470964311773	0.0
24	1.8853730870533315E-4	0.0	0.0	4.504297707951938	0.0
25	1.8853730870533315E-4	0.0	0.0	4.597105198162138	0.0
26	1.8853730870533315E-4	0.0	0.0	4.689771285390809	0.0
27	1.8853730870533315E-4	0.0	0.0	4.787999223226288	0.0
28	1.8853730870533315E-4	0.0	0.0	4.891270534069634	0.0
29	1.8853730870533315E-4	0.0	0.0	5.0067496356516505	0.0
30	1.8853730870533315E-4	0.0	0.0	5.145937303803363	0.0
31	2.3567163588166646E-4	0.0	0.0	5.263443181453962	0.0
32	2.3567163588166646E-4	0.0	0.0	5.383635715753612	0.0
33	2.3567163588166646E-4	0.0	0.0	5.504865205251141	0.0
34	2.3567163588166646E-4	0.0	0.0	5.625387679841025	0.0
35	2.3567163588166646E-4	0.0	0.0	5.758165079496756	0.0
36	2.3567163588166646E-4	0.0	0.0	5.892497911949306	0.0
37	2.3567163588166646E-4	0.0	0.0	6.025510983240919	0.0
38	2.8280596305799974E-4	0.0	0.0	6.17756632271177	0.0
39	2.8280596305799974E-4	0.0	0.0	6.3752005565621355	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGT	20	7.0346374E-4	45.0	42
CGTTTTT	26685	0.0	44.17369	1
CGACGGT	255	0.0	42.35294	28
CGAACGG	65	0.0	41.538464	2
GCGTAAG	115	0.0	41.086956	1
ACGGTCT	270	0.0	40.833336	30
GCGTTAG	100	0.0	40.5	1
GCGCGAC	230	0.0	40.108692	9
CGGGTTC	45	1.929584E-8	40.0	6
CGTTAGG	260	0.0	39.807693	2
GTTTTTT	30085	0.0	39.66761	2
TACGGGA	850	0.0	39.441177	4
GACGGTC	275	0.0	39.272728	29
TAGGGAC	2790	0.0	38.870968	5
TAGGGCG	425	0.0	38.64706	5
GCGCGAA	35	6.250788E-6	38.57143	34
GTTAGCG	140	0.0	38.57143	1
CGATTCG	35	6.250788E-6	38.57143	10
CACGACG	280	0.0	38.57143	26
GTAGGGA	3590	0.0	38.41922	4
>>END_MODULE