FastQCFastQC Report
Thu 26 May 2016
SRR1547332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547332_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences851785
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139111.633158602229436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22510.26426856542437355No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9640.11317409909777701No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGT253.889952E-545.013
AGCGTTG406.8121153E-945.01
CTATGCG207.032257E-445.01
CGGCGAT351.2114288E-745.06
GCGACTA207.032257E-445.013
ACGCCGA551.8189894E-1245.012
GTCGATC207.032257E-445.020
CGTATGG1000.045.02
ATTAGAC207.032257E-445.033
CTCCGAC253.889952E-545.017
ACGTTTC207.032257E-445.022
TTTACGG453.8380676E-1045.02
ACGTTAG253.889952E-545.01
GAGTCGG453.8380676E-1045.02
AGCCCGA406.8121153E-945.045
CGCATCG1100.045.021
CGCATCA253.889952E-545.030
GAATCGG253.889952E-545.02
CGACCGT207.032257E-445.020
CCTACGG253.889952E-545.043