##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547332_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	851785
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240491438567243	31.0	31.0	33.0	30.0	34.0
2	31.625621488990767	31.0	31.0	34.0	30.0	34.0
3	31.648184694494503	31.0	31.0	34.0	30.0	34.0
4	35.46632424848993	37.0	35.0	37.0	33.0	37.0
5	35.363098669265135	37.0	35.0	37.0	33.0	37.0
6	35.404413085461705	37.0	35.0	37.0	33.0	37.0
7	35.875222033729166	37.0	35.0	37.0	35.0	37.0
8	35.87250773375911	37.0	35.0	37.0	35.0	37.0
9	37.613620808067765	39.0	37.0	39.0	35.0	39.0
10	36.85603996313624	39.0	37.0	39.0	32.0	39.0
11	36.53825202369143	39.0	35.0	39.0	32.0	39.0
12	35.80235270637544	37.0	35.0	39.0	31.0	39.0
13	35.34587249129768	37.0	35.0	39.0	30.0	39.0
14	36.379648620250414	38.0	35.0	41.0	30.0	41.0
15	36.701940043555595	38.0	35.0	41.0	31.0	41.0
16	36.85244163726762	38.0	35.0	41.0	32.0	41.0
17	36.796331233820744	38.0	35.0	41.0	32.0	41.0
18	36.67206982982795	38.0	35.0	40.0	31.0	41.0
19	36.52002441930769	37.0	35.0	40.0	31.0	41.0
20	36.26612466760979	37.0	35.0	40.0	31.0	41.0
21	36.0855826294194	37.0	35.0	40.0	31.0	41.0
22	35.997385490470016	37.0	34.0	40.0	30.0	41.0
23	35.97134018561022	36.0	34.0	40.0	31.0	41.0
24	35.79221986768962	36.0	34.0	40.0	30.0	41.0
25	35.676288030430214	36.0	34.0	40.0	30.0	41.0
26	35.5070223119684	36.0	34.0	40.0	30.0	41.0
27	35.386428500149684	36.0	34.0	40.0	29.0	41.0
28	35.45027559771538	36.0	34.0	40.0	29.0	41.0
29	35.48902246458907	36.0	34.0	40.0	30.0	41.0
30	35.402009896863646	36.0	34.0	40.0	29.0	41.0
31	35.17594698192619	36.0	34.0	40.0	29.0	41.0
32	34.91714341060244	35.0	34.0	40.0	27.0	41.0
33	34.60542272991424	35.0	33.0	40.0	25.0	41.0
34	34.337635670973306	35.0	34.0	40.0	24.0	41.0
35	34.14260171287356	35.0	33.0	40.0	23.0	41.0
36	33.91066525003375	35.0	33.0	40.0	22.0	41.0
37	33.78830690843347	35.0	33.0	40.0	22.0	41.0
38	33.724939978985304	35.0	33.0	40.0	22.0	41.0
39	33.71349225450084	35.0	33.0	40.0	21.0	41.0
40	33.56040432738308	35.0	33.0	40.0	21.0	41.0
41	33.49493475466227	35.0	33.0	40.0	20.0	41.0
42	33.404301554969855	35.0	33.0	40.0	20.0	41.0
43	33.33767323913899	35.0	33.0	40.0	19.0	41.0
44	33.20585828583504	35.0	33.0	40.0	18.0	41.0
45	33.08268988066237	35.0	32.0	40.0	18.0	41.0
46	33.01738232065603	35.0	32.0	39.0	18.0	41.0
47	32.844734293278236	35.0	32.0	39.0	18.0	41.0
48	32.819335865271164	35.0	32.0	39.0	18.0	41.0
49	32.75783912607054	35.0	32.0	39.0	18.0	41.0
50	32.63449696813163	35.0	32.0	39.0	18.0	41.0
51	32.18465692633705	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	13.0
15	36.0
16	101.0
17	278.0
18	544.0
19	1109.0
20	1999.0
21	3305.0
22	4703.0
23	6995.0
24	10133.0
25	14595.0
26	19509.0
27	21613.0
28	22442.0
29	23713.0
30	26658.0
31	31078.0
32	37543.0
33	48014.0
34	79071.0
35	120603.0
36	65323.0
37	70354.0
38	96038.0
39	145966.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.39182422794485	23.699290313870282	28.649130942667455	17.259754515517415
2	32.171263875273695	25.458302271113016	29.847555427719435	12.522878425893857
3	27.87957054890612	24.09211244621589	35.44239450095975	12.585922503918242
4	23.25997757650111	27.706756986798315	34.77485515711125	14.258410279589334
5	21.543699407714385	30.236385942462007	35.28942162634937	12.930493023474234
6	18.58485415920684	41.138550221006476	30.900755472331632	9.375840147455051
7	84.39441877938681	3.856607007636904	10.458272920983582	1.2907012919926977
8	85.33127491092236	2.878895495929137	9.815270285341958	1.9745593078065473
9	82.49734381328622	4.357789817853097	11.228537717851337	1.9163286510093511
10	45.1589309508855	22.6930504763526	18.810967556366922	13.337051016394982
11	38.26094613077244	25.269639639110807	22.789671102449564	13.679743127667193
12	35.19691001837318	23.494543810938207	26.734328498388678	14.574217672299936
13	19.65660348562137	37.68627059645333	28.465633933445645	14.19149198447965
14	15.230016964374812	41.355271576747654	30.021777796040077	13.392933662837454
15	13.408195730143168	27.877222538551393	45.62583280992269	13.088748921382741
16	14.996037732526402	22.93513034392482	45.982143381252314	16.08668854229647
17	15.526805473212137	24.601043690602676	30.082239062674265	29.78991177351092
18	19.275051802978453	27.356903443944187	35.71934232229964	17.648702430777718
19	25.026150965325755	30.512746761213215	26.958680887782716	17.50242138567831
20	27.23621571171129	27.971377753775894	27.29679437886321	17.495612155649606
21	20.177861784370467	31.23851676185892	30.629560276360817	17.95406117740979
22	20.830608662984204	26.004449479622206	29.46987796216181	23.695063895231776
23	16.385472859935312	33.12889989844855	28.582447448593246	21.903179793022886
24	17.915319006556818	28.738824938217977	37.73358300510105	15.61227305012415
25	16.504047382848956	28.77885851476605	36.20397165951502	18.51312244286997
26	15.255492876723586	37.466614227768744	28.4201999330817	18.857692962425965
27	15.429832645561966	36.88043344271148	32.19216116743075	15.497572744295802
28	14.303492078400065	31.48646665531795	39.228913399508095	14.98112786677389
29	14.345521463749655	28.339663177914616	38.528736711728904	18.786078646606832
30	16.826077002999583	33.74325680776252	32.63887013741731	16.791796051820587
31	25.94175760315103	29.891580621870546	26.922404127802203	17.244257647176223
32	25.179593442007082	30.602440756763738	28.422078341365488	15.795887459863698
33	23.790745317186847	31.289938188627413	26.39598020627271	18.523336287913033
34	17.593876388994875	31.47860082062962	28.69644335131518	22.231079439060323
35	16.929859060678456	28.973860774726017	31.91885276214068	22.177427402454843
36	25.094478066648275	29.300351614550618	27.874991928714405	17.7301783900867
37	18.133449168510833	33.527474656163236	29.677559478037296	18.661516697288636
38	18.42295884524851	35.81572814736113	24.99140041207582	20.769912595314548
39	17.709515898965115	33.63313512212589	28.443210434558015	20.21413854435098
40	21.52409352125243	29.717240853032163	26.867343284983885	21.891322340731524
41	15.971636034914912	27.57573800900462	29.4759827890841	26.976643166996368
42	19.43142929260318	29.4177521322869	25.862629654196773	25.28818892091314
43	19.506213422401192	30.00428511889737	27.27448828049332	23.215013178208117
44	18.41802802350358	33.13852674090293	28.43147038278439	20.0119748528091
45	17.05477321154986	38.5209882775583	24.143064270913435	20.281174239978398
46	21.890265736071896	34.92430601618953	24.862142442048167	18.323285805690404
47	20.024067106135938	31.409686716718422	26.26930504763526	22.29694112951038
48	19.9362515188692	29.137517096450395	31.043397101381213	19.88283428329919
49	19.45103517906514	27.464559718708358	31.25084381622123	21.83356128600527
50	18.026027694782133	32.559742188463055	29.196921758424953	20.21730835832986
51	15.979854071156455	35.08655353170108	27.026186185481077	21.907406211661392
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2612.0
1	2195.5
2	1779.0
3	9980.5
4	18182.0
5	12171.5
6	6161.0
7	6867.0
8	7573.0
9	8516.0
10	9459.0
11	10497.5
12	11536.0
13	11623.5
14	11711.0
15	10873.5
16	10036.0
17	9158.0
18	8280.0
19	7526.0
20	6772.0
21	6531.5
22	6291.0
23	6634.0
24	6977.0
25	6843.0
26	8024.0
27	9339.0
28	10226.5
29	11114.0
30	12629.5
31	14145.0
32	16682.0
33	19219.0
34	21169.0
35	23119.0
36	24920.0
37	26721.0
38	33414.0
39	40107.0
40	48324.0
41	56541.0
42	66835.0
43	77129.0
44	76860.0
45	76591.0
46	75414.0
47	74237.0
48	71967.0
49	69697.0
50	68371.0
51	67045.0
52	61392.0
53	55739.0
54	46183.0
55	36627.0
56	30634.5
57	24642.0
58	21246.0
59	17850.0
60	16715.5
61	15581.0
62	12640.0
63	9699.0
64	7454.5
65	5210.0
66	4108.0
67	3006.0
68	2327.5
69	1649.0
70	1391.5
71	1134.0
72	798.0
73	462.0
74	424.0
75	335.5
76	285.0
77	236.5
78	188.0
79	153.5
80	119.0
81	84.5
82	50.0
83	37.0
84	24.0
85	27.5
86	31.0
87	21.0
88	11.0
89	8.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	851785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.438517799408192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95153859341639	22.211902325849348
2	7.8971150761061235	4.333702651594253
3	2.9004970277212516	2.3875601796678034
4	1.514641760077987	1.662380995745072
5	0.930636345348182	1.276764096330614
6	0.6530630503639964	1.0751449278928995
7	0.4731284693810783	0.908736074996363
8	0.34645033408438736	0.76048669267083
9	0.28512285853423935	0.7041013766018882
>10	2.6229203149645075	15.7865642385053
>50	0.6593590904393738	13.105494938823176
>100	0.7620884402382546	33.39665676404402
>500	0.0025789794931920635	0.4843753000149338
>1k	4.298299155320105E-4	0.2654805941888522
>5k	0.0	0.0
>10k+	4.298299155320105E-4	1.6406488430746884
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13911	1.633158602229436	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2251	0.26426856542437355	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	964	0.11317409909777701	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3480103547256643E-4	0.0	0.0	0.07079251219497879	0.0
2	2.3480103547256643E-4	0.0	0.0	0.24536708206883193	0.0
3	2.3480103547256643E-4	0.0	0.0	0.33975709832880363	0.0
4	2.3480103547256643E-4	0.0	0.0	0.6074302787675294	0.0
5	2.3480103547256643E-4	0.0	0.0	1.0830197761172127	0.0
6	2.3480103547256643E-4	0.0	0.0	1.6865758377994446	0.0
7	2.3480103547256643E-4	0.0	0.0	2.014475483836884	0.0
8	2.3480103547256643E-4	0.0	0.0	2.7530421409158414	0.0
9	2.3480103547256643E-4	0.0	0.0	2.9711723028698556	0.0
10	2.3480103547256643E-4	0.0	0.0	3.368925256960383	0.0
11	2.3480103547256643E-4	0.0	0.0	3.903684615249153	0.0
12	2.3480103547256643E-4	0.0	0.0	4.272322240941082	0.0
13	2.3480103547256643E-4	0.0	0.0	4.430225937296384	0.0
14	2.3480103547256643E-4	0.0	0.0	4.492096010143404	0.0
15	2.3480103547256643E-4	0.0	0.0	4.5752155767006935	0.0
16	2.3480103547256643E-4	0.0	0.0	4.79358053969018	0.0
17	2.3480103547256643E-4	0.0	0.0	5.063836531519104	0.0
18	2.3480103547256643E-4	0.0	0.0	5.392675381698433	0.0
19	2.3480103547256643E-4	0.0	0.0	5.5816902152538495	0.0
20	2.3480103547256643E-4	0.0	0.0	5.7561473846099664	0.0
21	2.3480103547256643E-4	0.0	0.0	6.046244063936322	0.0
22	2.3480103547256643E-4	0.0	0.0	6.32741830391472	0.0
23	2.3480103547256643E-4	0.0	0.0	6.655787552023105	0.0
24	2.3480103547256643E-4	0.0	0.0	6.872156706211074	0.0
25	2.3480103547256643E-4	0.0	0.0	7.069859178078976	0.0
26	2.3480103547256643E-4	0.0	0.0	7.248894967626807	0.0
27	2.3480103547256643E-4	0.0	0.0	7.427343754585958	0.0
28	2.3480103547256643E-4	0.0	0.0	7.635612273050124	0.0
29	2.3480103547256643E-4	0.0	0.0	7.840593577017675	0.0
30	2.3480103547256643E-4	0.0	0.0	8.088660870994442	0.0
31	2.3480103547256643E-4	0.0	0.0	8.327336123552305	0.0
32	2.3480103547256643E-4	0.0	0.0	8.564602569897334	0.0
33	2.3480103547256643E-4	0.0	0.0	8.801751615724625	0.0
34	2.3480103547256643E-4	0.0	0.0	9.028334614955652	0.0
35	2.3480103547256643E-4	0.0	0.0	9.301173418174773	0.0
36	2.3480103547256643E-4	0.0	0.0	9.544662091959825	0.0
37	2.3480103547256643E-4	0.0	0.0	9.794725194738108	0.0
38	2.3480103547256643E-4	0.0	0.0	10.038096468005424	0.0
39	2.3480103547256643E-4	0.0	0.0	10.284402754216146	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	25	3.889952E-5	45.0	13
AGCGTTG	40	6.8121153E-9	45.0	1
CTATGCG	20	7.032257E-4	45.0	1
CGGCGAT	35	1.2114288E-7	45.0	6
GCGACTA	20	7.032257E-4	45.0	13
ACGCCGA	55	1.8189894E-12	45.0	12
GTCGATC	20	7.032257E-4	45.0	20
CGTATGG	100	0.0	45.0	2
ATTAGAC	20	7.032257E-4	45.0	33
CTCCGAC	25	3.889952E-5	45.0	17
ACGTTTC	20	7.032257E-4	45.0	22
TTTACGG	45	3.8380676E-10	45.0	2
ACGTTAG	25	3.889952E-5	45.0	1
GAGTCGG	45	3.8380676E-10	45.0	2
AGCCCGA	40	6.8121153E-9	45.0	45
CGCATCG	110	0.0	45.0	21
CGCATCA	25	3.889952E-5	45.0	30
GAATCGG	25	3.889952E-5	45.0	2
CGACCGT	20	7.032257E-4	45.0	20
CCTACGG	25	3.889952E-5	45.0	43
>>END_MODULE