FastQCFastQC Report
Thu 26 May 2016
SRR1547331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547331_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2719797
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234790.8632629567574345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG207.0349884E-445.09
CGTTTTT129100.042.9783131
CGACGGT3050.039.83606728
TACGGGA12300.039.329274
GGGCGAT28700.038.8850177
TAGGGAC39200.038.6862265
AGGGATT64750.038.189196
TAGGGAT53150.037.972725
ATAGGGA46350.037.9611664
CGAATAT1550.037.74193614
ACGGGAT18100.037.541445
AGGGCGA14450.037.525956
TAAGGGA46550.037.266384
ACGGGAC10700.037.2196275
TTAGGGA47150.037.030754
TCACGAC3350.036.940325
GTACGGG8650.036.9364173
GTAGGGA47600.036.8697474
AGGGACC35200.036.818186
CGGGATC12500.036.726
Produced by FastQC (version 0.11.5)