##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547331_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2719797
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34034267998678	31.0	31.0	33.0	30.0	34.0
2	31.760415575132996	31.0	31.0	34.0	30.0	34.0
3	31.858828434622144	31.0	31.0	34.0	30.0	34.0
4	35.64006173990191	37.0	35.0	37.0	33.0	37.0
5	35.52576828344174	37.0	35.0	37.0	33.0	37.0
6	35.56443734587545	37.0	35.0	37.0	33.0	37.0
7	35.94536651080945	37.0	35.0	37.0	35.0	37.0
8	35.92454730996468	37.0	35.0	37.0	35.0	37.0
9	37.62292626986499	39.0	37.0	39.0	35.0	39.0
10	36.98903668178176	39.0	37.0	39.0	33.0	39.0
11	36.72972026956423	39.0	37.0	39.0	32.0	39.0
12	36.48668154277691	39.0	35.0	39.0	32.0	39.0
13	36.37975554793244	39.0	35.0	39.0	32.0	39.0
14	37.488703017173705	40.0	36.0	41.0	32.0	41.0
15	37.593156033336314	40.0	36.0	41.0	32.0	41.0
16	37.639627883992816	40.0	36.0	41.0	32.0	41.0
17	37.53082123408475	39.0	36.0	41.0	32.0	41.0
18	37.395938373341835	39.0	36.0	41.0	32.0	41.0
19	37.357843986150435	39.0	36.0	41.0	32.0	41.0
20	37.20442371250501	39.0	35.0	41.0	32.0	41.0
21	37.096704276091195	39.0	35.0	41.0	31.0	41.0
22	37.04224543228778	39.0	35.0	41.0	31.0	41.0
23	36.94006207080896	39.0	35.0	41.0	31.0	41.0
24	36.81215142159507	39.0	35.0	41.0	31.0	41.0
25	36.73988205737413	38.0	35.0	40.0	31.0	41.0
26	36.64172436398746	38.0	35.0	40.0	31.0	41.0
27	36.54268609017511	38.0	35.0	40.0	30.0	41.0
28	36.61966058496277	39.0	35.0	40.0	30.0	41.0
29	36.60888992818214	39.0	35.0	41.0	30.0	41.0
30	36.54683823829499	39.0	35.0	41.0	30.0	41.0
31	36.44374414708157	39.0	35.0	41.0	30.0	41.0
32	36.331924404652256	39.0	35.0	41.0	30.0	41.0
33	36.116181465013746	38.0	35.0	41.0	29.0	41.0
34	35.892104079826545	38.0	35.0	41.0	28.0	41.0
35	35.72970997467826	38.0	35.0	40.0	27.0	41.0
36	35.614162748175694	38.0	35.0	40.0	27.0	41.0
37	35.533886904059386	38.0	35.0	40.0	26.0	41.0
38	35.437859884395785	38.0	34.0	40.0	26.0	41.0
39	35.3886323133675	38.0	34.0	40.0	26.0	41.0
40	35.24304019748533	38.0	34.0	40.0	25.0	41.0
41	35.15756249455382	38.0	34.0	40.0	25.0	41.0
42	35.101465660856306	38.0	34.0	40.0	24.0	41.0
43	35.05852569143947	38.0	34.0	40.0	24.0	41.0
44	34.93950467626812	38.0	34.0	40.0	24.0	41.0
45	34.8451752097675	38.0	34.0	40.0	24.0	41.0
46	34.77502916577965	38.0	34.0	40.0	24.0	41.0
47	34.63446646937253	38.0	33.0	40.0	23.0	41.0
48	34.57785967114457	38.0	33.0	40.0	23.0	41.0
49	34.458313249113814	37.0	33.0	40.0	24.0	41.0
50	34.39762011650134	37.0	33.0	40.0	23.0	41.0
51	33.95568198656002	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	4.0
12	17.0
13	13.0
14	39.0
15	92.0
16	207.0
17	615.0
18	1234.0
19	2398.0
20	4013.0
21	6359.0
22	9758.0
23	14371.0
24	21081.0
25	30348.0
26	41485.0
27	49399.0
28	53624.0
29	59295.0
30	68394.0
31	82475.0
32	100499.0
33	127172.0
34	185697.0
35	231056.0
36	221262.0
37	283286.0
38	423049.0
39	702333.0
40	217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.20755188714452	22.883766692881856	28.37344110608255	15.535240313891075
2	32.53706067033679	25.534920437076735	28.773544496151736	13.154474396434734
3	28.5092232986506	25.67544563068494	32.62379508470669	13.191535985957776
4	25.77593107132628	27.676293488080177	31.6746433649276	14.87313207566594
5	23.148639402131852	31.94186919097271	30.574524495762002	14.33496691113344
6	21.663565332265605	40.92195116032557	27.42053910641125	9.993944400997574
7	87.8413719847474	3.136042873788007	7.54104074679103	1.4815443946735731
8	88.26353584477077	2.8200266416942146	7.3150312321103375	1.6014062814246797
9	82.94953630730528	6.142701091294681	8.519201984559876	2.388560616840154
10	34.93249680031267	41.95967566697074	14.05965224610513	9.048175286611464
11	27.158644560605072	24.429543822572054	30.688613892875093	17.72319772394778
12	27.305346685800448	22.393877190099115	32.16173854151615	18.13903758258429
13	24.392371930699237	24.341449012554982	31.884328131842192	19.38185092490359
14	19.239928568198287	28.324430095334318	32.29667508273595	20.138966253731436
15	19.016860449511487	29.02606334222738	34.547798971761495	17.409277236499634
16	24.53730921829828	27.31575187412884	32.013198043824595	16.133740863748287
17	23.35402237740537	27.683941117664297	29.42322533630267	19.53881116862766
18	23.025284607637996	28.289170110857537	31.107615752205035	17.57792952929943
19	24.20305633104235	29.51815889200554	28.64210086267468	17.636683914277427
20	25.814831033345502	29.022092457635623	28.692214896920614	16.470861612098258
21	25.80100647217421	26.351157825381822	31.185231838993865	16.662603863450105
22	24.200078167598537	23.74441916069471	32.066510846213895	19.988991825492857
23	21.62856271993829	26.52039839738039	32.44547295257698	19.405565930104345
24	21.3355629114967	26.799978086599847	32.92561908112995	18.938839920773496
25	21.54822584185511	29.23644669069052	30.103055485391007	19.112271982063366
26	21.37023461677471	30.50775480670065	28.259131104269912	19.86287947225473
27	19.68448380522517	30.12938097953634	31.306454121392147	18.879681093846344
28	19.015610356214086	29.856125291703755	32.0102566478307	19.118007704251458
29	20.394757402850285	27.983853206691528	32.095777736353114	19.52561165410507
30	21.232172842311392	27.226848180213448	31.386349790076245	20.15462918739891
31	22.58834023274531	27.964219388432298	29.706408235614646	19.741032143207747
32	23.378840406103837	28.413333789249712	29.167470954633746	19.040354850012704
33	22.697870466067872	27.676881767278953	29.342741388419796	20.282506378233375
34	21.049401848740917	28.057498408888605	31.020954872734986	19.87214486963549
35	20.878837648545094	28.568639497727222	30.708247711134323	19.84427514259336
36	22.671949413871697	28.82803385693859	28.63437969819071	19.865637030999007
37	21.55760154158564	29.80490823396011	28.99429626549334	19.643193958960907
38	21.97730198246413	29.878553436157183	28.01911319116831	20.12503139021037
39	21.47391882555941	28.238614867212515	29.142505856135585	21.14496045109249
40	22.335490479620354	27.745048619437405	29.82957919285888	20.08988170808336
41	20.17161574926364	28.082206135237296	30.043896658463847	21.702281457035212
42	21.316664442235947	28.827224973040266	28.29519997264502	21.560910612078768
43	21.231878702712002	27.835349476449895	29.447418318352437	21.485353502485662
44	21.22592237582437	28.758837516182272	29.1319535980075	20.883286509985854
45	20.80964130778878	29.095075845734076	28.63599746598735	21.459285380489792
46	21.32081916407732	29.01109899010845	28.529224791409064	21.138857054405165
47	20.93064298548752	28.71148104068061	29.639381174403823	20.718494799428044
48	21.142975008796615	27.99392748797061	29.894694346673667	20.96840315655911
49	21.418657348324157	27.509884009725727	29.642322570397717	21.4291360715524
50	20.434208876618364	29.028747366071805	29.687031789504882	20.85001196780495
51	19.875784847177933	28.51359862519151	29.651073223479546	21.95954330415101
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2717.0
1	3473.5
2	4230.0
3	21485.0
4	38740.0
5	26906.5
6	15073.0
7	15210.0
8	15347.0
9	15859.5
10	16372.0
11	16851.5
12	17331.0
13	16577.5
14	15824.0
15	14867.5
16	13911.0
17	13973.5
18	14036.0
19	13689.5
20	13343.0
21	14905.0
22	16467.0
23	17294.0
24	18121.0
25	22608.5
26	34073.5
27	41051.0
28	46680.0
29	52309.0
30	58453.5
31	64598.0
32	75095.0
33	85592.0
34	98441.5
35	111291.0
36	117035.0
37	122779.0
38	131522.0
39	140265.0
40	150754.5
41	161244.0
42	171120.0
43	180996.0
44	185939.0
45	190882.0
46	195239.5
47	199597.0
48	196615.0
49	193633.0
50	184294.5
51	174956.0
52	165327.5
53	155699.0
54	144243.0
55	132787.0
56	123709.5
57	114632.0
58	109817.0
59	105002.0
60	94431.0
61	83860.0
62	72462.0
63	61064.0
64	52414.5
65	43765.0
66	35644.5
67	27524.0
68	23200.0
69	18876.0
70	15087.5
71	11299.0
72	9709.5
73	8120.0
74	6316.5
75	3564.5
76	2616.0
77	1892.5
78	1169.0
79	875.0
80	581.0
81	418.0
82	255.0
83	184.5
84	114.0
85	85.5
86	57.0
87	49.0
88	41.0
89	24.5
90	8.0
91	7.0
92	6.0
93	6.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2719797.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.030742311736077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.98698853877923	16.222294980238104
2	6.199658113957994	2.4836750820311253
3	2.096774643095887	1.2599985768490831
4	1.1324753125666105	0.9073728464169819
5	0.7281657076128522	0.7292849824718
6	0.5613088620617286	0.6746059903951378
7	0.4166977049452449	0.5842735044755023
8	0.3368920667632441	0.5398558540962184
9	0.2888496181739155	0.5207285041636555
>10	4.295889770909103	21.681953221337043
>50	2.0634951659085043	29.848965095993368
>100	0.8898383783920758	23.17138266083413
>500	0.0018538230210806356	0.24552637951180187
>1k	9.269115105403178E-4	0.25822601983137783
>5k	0.0	0.0
>10k+	1.8538230210806355E-4	0.8718563013547485
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23479	0.8632629567574345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1030234977095717E-4	0.0	0.0	0.020700074307016297	0.0
2	1.1030234977095717E-4	0.0	0.0	0.056217430933264506	0.0
3	1.4706979969460955E-4	0.0	0.0	0.0907420664115741	0.0
4	1.4706979969460955E-4	0.0	0.0	0.1590192209197966	0.0
5	1.4706979969460955E-4	0.0	0.0	0.3043609504679945	0.0
6	1.4706979969460955E-4	0.0	0.0	0.5549678891476092	0.0
7	1.4706979969460955E-4	0.0	0.0	0.6894264535184059	0.0
8	1.4706979969460955E-4	0.0	0.0	0.9287090176215357	0.0
9	1.4706979969460955E-4	0.0	0.0	1.0458133456283687	0.0
10	1.4706979969460955E-4	0.0	0.0	1.2186571277194584	0.0
11	1.4706979969460955E-4	0.0	0.0	1.3987808648954316	0.0
12	1.4706979969460955E-4	0.0	0.0	1.5509245726795051	0.0
13	1.4706979969460955E-4	0.0	0.0	1.6205988902848265	0.0
14	1.4706979969460955E-4	0.0	0.0	1.665491946641606	0.0
15	1.4706979969460955E-4	0.0	0.0	1.7064508858565548	0.0
16	1.4706979969460955E-4	0.0	0.0	1.7762722732615706	0.0
17	1.4706979969460955E-4	0.0	0.0	1.863705269180016	0.0
18	1.4706979969460955E-4	0.0	0.0	1.9626832443744882	0.0
19	1.4706979969460955E-4	0.0	0.0	2.035151888174007	0.0
20	1.4706979969460955E-4	0.0	0.0	2.1185404646008505	0.0
21	1.4706979969460955E-4	0.0	0.0	2.2152388579000566	0.0
22	1.4706979969460955E-4	0.0	0.0	2.3303209761610884	0.0
23	1.4706979969460955E-4	0.0	0.0	2.449594583713417	0.0
24	1.4706979969460955E-4	0.0	0.0	2.543756022967891	0.0
25	1.4706979969460955E-4	0.0	0.0	2.627475506444047	0.0
26	1.4706979969460955E-4	0.0	0.0	2.7053857328322666	0.0
27	1.4706979969460955E-4	0.0	0.0	2.788075727710561	0.0
28	1.8383724961826194E-4	0.0	0.0	2.879222236071295	0.0
29	1.8383724961826194E-4	0.0	0.0	2.9835682589546204	0.0
30	1.8383724961826194E-4	0.0	0.0	3.1131735199354953	0.0
31	2.2060469954191434E-4	0.0	0.0	3.2235494046063	0.0
32	2.2060469954191434E-4	0.0	0.0	3.3355430570737448	0.0
33	2.2060469954191434E-4	0.0	0.0	3.4494853843871436	0.0
34	2.2060469954191434E-4	0.0	0.0	3.5626555952521457	0.0
35	2.2060469954191434E-4	0.0	0.0	3.685642715246763	0.0
36	2.2060469954191434E-4	0.0	0.0	3.809181346990235	0.0
37	2.2060469954191434E-4	0.0	0.0	3.9332714904825616	0.0
38	2.2060469954191434E-4	0.0	0.0	4.071002357896564	0.0
39	2.2060469954191434E-4	0.0	0.0	4.288187684595578	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	20	7.0349884E-4	45.0	9
CGTTTTT	12910	0.0	42.978313	1
CGACGGT	305	0.0	39.836067	28
TACGGGA	1230	0.0	39.32927	4
GGGCGAT	2870	0.0	38.885017	7
TAGGGAC	3920	0.0	38.686226	5
AGGGATT	6475	0.0	38.18919	6
TAGGGAT	5315	0.0	37.97272	5
ATAGGGA	4635	0.0	37.961166	4
CGAATAT	155	0.0	37.741936	14
ACGGGAT	1810	0.0	37.54144	5
AGGGCGA	1445	0.0	37.52595	6
TAAGGGA	4655	0.0	37.26638	4
ACGGGAC	1070	0.0	37.219627	5
TTAGGGA	4715	0.0	37.03075	4
TCACGAC	335	0.0	36.9403	25
GTACGGG	865	0.0	36.936417	3
GTAGGGA	4760	0.0	36.869747	4
AGGGACC	3520	0.0	36.81818	6
CGGGATC	1250	0.0	36.72	6
>>END_MODULE