FastQCFastQC Report
Thu 26 May 2016
SRR1547330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547330_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3798517
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT612101.6114183508985216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38830.10222410482827904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT303500.043.0650751
TACGTAG2450.038.571431
CGGTCTA3200.037.9687531
TAGGGAC46550.037.9430735
TACGGGA18150.037.685954
TTAGGGA54800.037.034674
TAGGGAT65200.036.9593545
CATACGA15350.036.9381118
ATAGGGA59900.036.886484
GGGCGAT43350.036.799317
GGCGATA9050.036.7955788
AGGGATT66550.036.7505656
TAAGGGA50250.036.7164154
AAGGGAT64500.036.5930255
GTTTTTT371900.036.408982
TCGATAG1800.036.251
AGGGATC41450.036.2062726
ACGGGAT19900.036.067845
ACGGGAG17250.036.05
GTAGGGA57350.035.6625984
Produced by FastQC (version 0.11.5)