FastQCFastQC Report
Thu 26 May 2016
SRR1547329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547329_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1276041
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293752.3020420190260347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG253.8910475E-545.0000041
CGTTTTT144700.043.989291
TATAGCG700.041.7857131
TACGGGA4200.040.7142874
GGCGATA4050.040.08
TAGCCGT850.039.70588344
GCTATCG403.4586265E-739.3750041
GCGATAT1150.039.1304369
ACGGGTA2250.039.05
CGGGTAT1850.038.918926
GCCGCAT700.038.57142619
AGGGCGA7250.038.4827586
GTTTTTT168800.038.4019552
AGGGATT24050.037.9833646
TAGGGTA7550.037.5496675
CGAACGG900.037.5000042
GGCACCG4500.037.58
TAACGCG301.1399925E-437.4999961
CGTCCAA301.1399925E-437.49999632
CGGGATA4100.037.3170746
Produced by FastQC (version 0.11.5)