##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547329_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1276041
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30471042858341	31.0	31.0	33.0	30.0	34.0
2	31.709038345946563	31.0	31.0	34.0	30.0	34.0
3	31.66396064076311	31.0	31.0	34.0	30.0	34.0
4	35.5371865010607	37.0	35.0	37.0	33.0	37.0
5	35.4706267275111	37.0	35.0	37.0	33.0	37.0
6	35.5272087652356	37.0	35.0	37.0	33.0	37.0
7	35.93142540090796	37.0	35.0	37.0	35.0	37.0
8	35.92314196800886	37.0	35.0	37.0	35.0	37.0
9	37.628353634405165	39.0	37.0	39.0	35.0	39.0
10	36.92752897438248	39.0	37.0	39.0	32.0	39.0
11	36.71867675098214	39.0	37.0	39.0	32.0	39.0
12	36.595834303129756	39.0	35.0	39.0	32.0	39.0
13	36.54006336786984	39.0	35.0	39.0	32.0	39.0
14	37.68790187776098	40.0	36.0	41.0	32.0	41.0
15	37.75534328442425	40.0	36.0	41.0	32.0	41.0
16	37.77654401386789	40.0	36.0	41.0	32.0	41.0
17	37.67096981993525	40.0	36.0	41.0	32.0	41.0
18	37.45022534542385	39.0	36.0	41.0	32.0	41.0
19	37.27797852890307	39.0	36.0	41.0	32.0	41.0
20	37.021499309191476	39.0	35.0	41.0	32.0	41.0
21	36.90935714448047	38.0	35.0	41.0	31.0	41.0
22	36.835339930300044	38.0	35.0	40.0	31.0	41.0
23	36.74308349026403	38.0	35.0	40.0	31.0	41.0
24	36.590354855369064	38.0	35.0	40.0	31.0	41.0
25	36.49147715473092	38.0	35.0	40.0	31.0	41.0
26	36.36761671450996	38.0	35.0	40.0	30.0	41.0
27	36.28429102199694	38.0	35.0	40.0	30.0	41.0
28	36.29376877388736	38.0	35.0	40.0	30.0	41.0
29	36.26284265160759	38.0	35.0	40.0	30.0	41.0
30	36.15707645757464	38.0	35.0	40.0	30.0	41.0
31	35.9940621030202	38.0	35.0	40.0	30.0	41.0
32	35.75120862103961	38.0	35.0	40.0	28.0	41.0
33	35.3599829472564	38.0	34.0	40.0	25.0	41.0
34	34.93413456150704	38.0	34.0	40.0	23.0	41.0
35	34.63997708537578	38.0	34.0	40.0	22.0	41.0
36	34.43185446235662	38.0	33.0	40.0	21.0	41.0
37	34.32142149037531	38.0	33.0	40.0	20.0	41.0
38	34.20135795009721	38.0	33.0	40.0	19.0	41.0
39	34.13591804652045	38.0	33.0	40.0	18.0	41.0
40	33.987346017878735	38.0	33.0	40.0	18.0	41.0
41	33.875052604109115	38.0	33.0	40.0	18.0	41.0
42	33.78806950560366	38.0	33.0	40.0	18.0	41.0
43	33.7298104057785	38.0	33.0	40.0	18.0	41.0
44	33.58971302646231	37.0	33.0	40.0	17.0	41.0
45	33.49955604874765	37.0	33.0	40.0	17.0	41.0
46	33.41916834960632	37.0	32.0	40.0	17.0	41.0
47	33.28793432185957	37.0	32.0	40.0	17.0	41.0
48	33.216720309143675	37.0	32.0	40.0	17.0	41.0
49	33.097242956926934	36.0	32.0	40.0	15.0	41.0
50	33.02506972738337	36.0	32.0	40.0	15.0	41.0
51	32.61296384677295	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	2.0
13	9.0
14	20.0
15	45.0
16	128.0
17	362.0
18	786.0
19	1497.0
20	2573.0
21	3968.0
22	6157.0
23	9083.0
24	13567.0
25	21001.0
26	29733.0
27	34836.0
28	34533.0
29	33758.0
30	35893.0
31	40223.0
32	47253.0
33	58055.0
34	80903.0
35	104619.0
36	106637.0
37	130102.0
38	187989.0
39	292243.0
40	62.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.13688431641303	22.396929252273242	25.895641284253408	21.570545147060322
2	37.55459268158312	23.376678335570723	27.1512435728946	11.917485409951562
3	26.48950934962121	23.689912784934027	37.44354609295469	12.377031772490069
4	23.85832430149188	25.84987472973047	37.22458761121312	13.06721335756453
5	21.258642943291008	30.21634884772511	35.56766592922955	12.957342279754332
6	19.794426668108628	38.451742538053246	32.5495027197402	9.204328074097932
7	81.99329018424957	2.9566448100021865	13.685453680563556	1.3646113251846923
8	82.90344902710807	2.4060355427451	13.507716444847775	1.1827989852990617
9	78.08385467238122	5.137452479975173	14.786593847689847	1.9920989999537633
10	30.736238098932557	35.26007393179373	24.489573610879276	9.51411435839444
11	24.280959624338088	25.029524913384442	34.21065624066939	16.478859221608083
12	24.21426897725073	23.053099391007027	35.43977035220655	17.29286127953569
13	22.238783863527896	23.764753640361086	35.9261967287885	18.070265767322525
14	18.341495296781215	27.339011834259242	36.21913402469043	18.10035884426911
15	18.236326262243924	27.868618641564026	37.78891117134951	16.106143924842538
16	21.993415572070177	26.77453153934709	36.72656286122468	14.505490027358055
17	21.29351643089838	26.27423413511008	34.26872647508975	18.16352295890179
18	20.565796867028567	27.33673918001067	34.971290107449526	17.126173845511232
19	22.3048475715122	27.525212747866252	33.21625245583802	16.95368722478353
20	23.75197975613636	27.789937784130757	33.102384641245855	15.355697818487023
21	23.950092512701392	25.508819857669152	35.25740944060575	15.283678189023705
22	22.393559454594328	23.379421194146584	35.67197292250014	18.55504642875895
23	20.1198080625936	26.57955347829733	35.5142193706942	17.78641908841487
24	19.76738991928943	26.990120223409747	36.132302958917464	17.110186898383358
25	20.533196033669764	28.209595146237465	33.86717197958372	17.390036840509044
26	20.05264721117895	30.02011690846924	32.292692789651745	17.634543090700063
27	18.607239109088187	28.793745655507934	35.06917097491382	17.529844260490062
28	17.97802735178572	28.772272991228338	36.24664097783692	17.003058679149024
29	19.33911214451573	27.270518737250605	35.44564790629769	17.94472121193598
30	19.875223445014697	26.93377407152278	35.03680524371866	18.154197239743862
31	20.960298297625233	27.836096175593106	33.37831621397745	17.825289312804212
32	21.69044725051938	27.76956226328151	33.31397658852654	17.22601389767257
33	20.896899080828906	27.95513623778546	32.77136079483339	18.376603886552235
34	19.711200502178222	28.98488371455149	32.86783104931582	18.436084733954473
35	20.04551577888171	28.796723616247437	32.17310415574421	18.984656449126636
36	21.41435894301202	30.057184682937304	30.82244222560247	17.706014148448208
37	20.548164204755174	30.572685360423375	30.521041251809304	18.358109183012143
38	20.056408845797275	31.186223640149496	29.911656443640915	18.845711070412314
39	20.120121532145127	29.89958786590713	30.00412996134137	19.976160640606373
40	21.427132827236743	29.22304220632409	30.902533696017603	18.447291270421562
41	19.3637195043106	29.852959270117495	31.165613017136597	19.617708208435307
42	20.629823022927944	30.587340061957256	29.264263452349887	19.51857346276491
43	20.554511963173596	29.692227757572056	29.97615280386759	19.777107475386764
44	20.426929855702127	30.04801569855514	30.12144594100033	19.403608504742405
45	20.323876740637644	30.007969963347573	29.590898725040965	20.077254570973814
46	20.55819523040404	30.29244358135828	29.38157943200885	19.767781756228835
47	20.566815643071028	29.961263000170057	29.98022790803744	19.491693448721474
48	19.92036306043458	30.207414965506597	30.421671403975264	19.450550570083564
49	20.45686619787295	29.684155916620234	30.43703141200009	19.42194647350673
50	19.33221581438214	29.8078980220855	31.233714277205827	19.62617188632654
51	19.09217650530038	30.472375103934745	30.67221194303318	19.763236447731693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1411.0
1	1770.0
2	2129.0
3	22720.0
4	43311.0
5	29330.0
6	15349.0
7	15242.0
8	15135.0
9	15839.0
10	16543.0
11	16855.5
12	17168.0
13	16970.0
14	16772.0
15	15965.0
16	15158.0
17	14521.0
18	13884.0
19	13098.5
20	12313.0
21	11726.0
22	11139.0
23	11589.0
24	12039.0
25	13019.0
26	15582.0
27	17165.0
28	19982.0
29	22799.0
30	26170.0
31	29541.0
32	32833.5
33	36126.0
34	39338.5
35	42551.0
36	47037.0
37	51523.0
38	55693.0
39	59863.0
40	66170.5
41	72478.0
42	74956.0
43	77434.0
44	79144.0
45	80854.0
46	84941.0
47	89028.0
48	88376.0
49	87724.0
50	82964.0
51	78204.0
52	73491.5
53	68779.0
54	62850.0
55	56921.0
56	53201.5
57	49482.0
58	46246.5
59	43011.0
60	38733.5
61	34456.0
62	30163.5
63	25871.0
64	21474.5
65	17078.0
66	13894.5
67	10711.0
68	8926.5
69	7142.0
70	5826.5
71	4511.0
72	3722.0
73	2933.0
74	2387.0
75	1417.5
76	994.0
77	683.0
78	372.0
79	252.0
80	132.0
81	106.0
82	80.0
83	52.0
84	24.0
85	24.0
86	24.0
87	19.5
88	15.0
89	12.5
90	10.0
91	9.0
92	8.0
93	6.5
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1276041.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.42919300047168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.30274568107417	22.16099036113767
2	6.666925077910019	3.924044496749973
3	3.098068352059673	2.735209543842521
4	2.066893668612009	2.4330805074014314
5	1.3904149778941821	2.045939536759723
6	1.0855379230276039	1.916790302766632
7	0.9031667397906917	1.8605627807834937
8	0.7306878868595954	1.720284387639828
9	0.6834587947366767	1.8102276700357876
>10	7.642833001393015	47.96009635514903
>50	0.40354542759824485	7.322389042729288
>100	0.02384687234808665	1.3510721300449904
>500	8.038271554430001E-4	0.18538406517131406
>1k	8.038271554430001E-4	0.25761367116745343
>5k	0.0	0.0
>10k+	2.679423851476667E-4	2.316315148620736
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29375	2.3020420190260347	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.836738788173734E-5	0.0	0.0	0.02155103166747777	0.0
2	7.836738788173734E-5	0.0	0.0	0.07076575125720883	0.0
3	7.836738788173734E-5	0.0	0.0	0.1213127164409294	0.0
4	7.836738788173734E-5	0.0	0.0	0.1861225462191262	0.0
5	7.836738788173734E-5	0.0	0.0	0.3446597719038808	0.0
6	7.836738788173734E-5	0.0	0.0	0.6456689087576339	0.0
7	7.836738788173734E-5	0.0	0.0	0.8498943215774415	0.0
8	7.836738788173734E-5	0.0	0.0	1.102158943168754	0.0
9	7.836738788173734E-5	0.0	0.0	1.242985139192236	0.0
10	7.836738788173734E-5	0.0	0.0	1.380206435373158	0.0
11	7.836738788173734E-5	0.0	0.0	1.5030864995717221	0.0
12	7.836738788173734E-5	0.0	0.0	1.595638384660054	0.0
13	7.836738788173734E-5	0.0	0.0	1.654727395122884	0.0
14	7.836738788173734E-5	0.0	0.0	1.697986193233603	0.0
15	7.836738788173734E-5	0.0	0.0	1.7287845766711258	0.0
16	7.836738788173734E-5	0.0	0.0	1.7741592942546518	0.0
17	7.836738788173734E-5	0.0	0.0	1.8253331985414263	0.0
18	7.836738788173734E-5	0.0	0.0	1.8991552779260228	0.0
19	7.836738788173734E-5	0.0	0.0	1.9472728540854094	0.0
20	7.836738788173734E-5	0.0	0.0	1.9989169626994745	0.0
21	7.836738788173734E-5	0.0	0.0	2.061454138229101	0.0
22	7.836738788173734E-5	0.0	0.0	2.13088764389232	0.0
23	7.836738788173734E-5	0.0	0.0	2.2086280926710034	0.0
24	7.836738788173734E-5	0.0	0.0	2.2699897573824037	0.0
25	7.836738788173734E-5	0.0	0.0	2.3297057069482876	0.0
26	7.836738788173734E-5	0.0	0.0	2.390205330392989	0.0
27	7.836738788173734E-5	0.0	0.0	2.450078014734636	0.0
28	7.836738788173734E-5	0.0	0.0	2.5164551922704677	0.0
29	7.836738788173734E-5	0.0	0.0	2.5917662520248173	0.0
30	7.836738788173734E-5	0.0	0.0	2.6836911980100955	0.0
31	7.836738788173734E-5	0.0	0.0	2.7597075642553803	0.0
32	1.5673477576347468E-4	0.0	0.0	2.8419933215312048	0.0
33	1.5673477576347468E-4	0.0	0.0	2.927257039546535	0.0
34	1.5673477576347468E-4	0.0	0.0	3.0067215708586166	0.0
35	1.5673477576347468E-4	0.0	0.0	3.0956685561043886	0.0
36	2.3510216364521202E-4	0.0	0.0	3.186809828210849	0.0
37	2.3510216364521202E-4	0.0	0.0	3.2824180414265687	0.0
38	2.3510216364521202E-4	0.0	0.0	3.3871952390244515	0.0
39	2.3510216364521202E-4	0.0	0.0	3.5252785764720724	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	25	3.8910475E-5	45.000004	1
CGTTTTT	14470	0.0	43.98929	1
TATAGCG	70	0.0	41.785713	1
TACGGGA	420	0.0	40.714287	4
GGCGATA	405	0.0	40.0	8
TAGCCGT	85	0.0	39.705883	44
GCTATCG	40	3.4586265E-7	39.375004	1
GCGATAT	115	0.0	39.130436	9
ACGGGTA	225	0.0	39.0	5
CGGGTAT	185	0.0	38.91892	6
GCCGCAT	70	0.0	38.571426	19
AGGGCGA	725	0.0	38.482758	6
GTTTTTT	16880	0.0	38.401955	2
AGGGATT	2405	0.0	37.983364	6
TAGGGTA	755	0.0	37.549667	5
CGAACGG	90	0.0	37.500004	2
GGCACCG	450	0.0	37.5	8
TAACGCG	30	1.1399925E-4	37.499996	1
CGTCCAA	30	1.1399925E-4	37.499996	32
CGGGATA	410	0.0	37.317074	6
>>END_MODULE