FastQCFastQC Report
Thu 26 May 2016
SRR1547328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547328_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences32002
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.6437097681394913No Hit
TGACTTGGGGTATATGAGTTCATATGCAGTGAGCTCATGAGTACCACAGCT1590.49684394725329667No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG640.19998750078120117No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG430.13436660208736956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.12499218798825074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAT453.601599E-1045.0000045
GGATGTA253.80617E-545.08
TAGCGGG206.9308653E-445.03
GCGGGAC206.9308653E-445.05
ATGGGAT406.4646883E-945.05
GTATGGG206.9308653E-445.03
CAATGGG253.80617E-545.03
GGGTATA206.9308653E-445.08
TATGAGT206.9308653E-445.013
GTGAGCT206.9308653E-445.029
TTGGGAT351.16318915E-745.05
ACGGGGG206.9308653E-445.05
ACGGGAT206.9308653E-445.05
AGTGAGC206.9308653E-445.028
GGGATAT253.80617E-545.07
ACTAGGG206.9308653E-445.03
GGGAATA206.9308653E-445.07
AACAGGG253.80617E-545.03
TGAGCTC206.9308653E-445.030
GGTATAT206.9308653E-445.09