##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547328_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	32002
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312261733641648	31.0	31.0	33.0	30.0	34.0
2	31.723923504780952	31.0	31.0	34.0	30.0	34.0
3	31.885163427285793	31.0	31.0	34.0	30.0	34.0
4	35.63824135991501	37.0	35.0	37.0	33.0	37.0
5	35.50349978126367	37.0	35.0	37.0	33.0	37.0
6	35.54215361539904	37.0	35.0	37.0	33.0	37.0
7	35.90409974376602	37.0	35.0	37.0	35.0	37.0
8	35.86663333541654	37.0	35.0	37.0	35.0	37.0
9	37.55393412911693	39.0	37.0	39.0	35.0	39.0
10	36.91744265983376	39.0	37.0	39.0	32.0	39.0
11	36.62649209424411	39.0	35.0	39.0	32.0	39.0
12	36.15261546153366	38.0	35.0	39.0	32.0	39.0
13	35.966189613149176	38.0	35.0	39.0	31.0	39.0
14	37.080901193675395	39.0	35.0	41.0	31.0	41.0
15	37.27529529404412	39.0	35.0	41.0	32.0	41.0
16	37.32929191925505	39.0	35.0	41.0	32.0	41.0
17	37.22817323917255	39.0	35.0	41.0	32.0	41.0
18	37.126304605962126	39.0	35.0	41.0	32.0	41.0
19	37.12036747703269	39.0	35.0	41.0	32.0	41.0
20	36.98384475970252	39.0	35.0	41.0	31.0	41.0
21	36.83157302668583	39.0	35.0	41.0	31.0	41.0
22	36.750296856446475	38.0	35.0	41.0	31.0	41.0
23	36.65808386975814	38.0	35.0	40.0	31.0	41.0
24	36.52952940441222	38.0	35.0	40.0	31.0	41.0
25	36.46853321667396	38.0	35.0	40.0	31.0	41.0
26	36.31073057933879	38.0	35.0	40.0	30.0	41.0
27	36.21314292856697	38.0	35.0	40.0	30.0	41.0
28	36.315917755140305	38.0	35.0	40.0	30.0	41.0
29	36.388069495656524	38.0	35.0	40.0	30.0	41.0
30	36.32922942316105	38.0	35.0	40.0	30.0	41.0
31	36.240828698206364	38.0	35.0	40.0	30.0	41.0
32	36.123461033685395	38.0	35.0	40.0	30.0	41.0
33	35.982907318292604	38.0	35.0	40.0	29.0	41.0
34	35.812605462158615	38.0	35.0	40.0	29.0	41.0
35	35.68951940503719	38.0	34.0	40.0	28.0	41.0
36	35.55996500218736	38.0	34.0	40.0	27.0	41.0
37	35.441878632585464	38.0	34.0	40.0	27.0	41.0
38	35.425035935254044	38.0	34.0	40.0	27.0	41.0
39	35.434035372789204	38.0	34.0	40.0	27.0	41.0
40	35.224110993062936	38.0	34.0	40.0	26.0	41.0
41	35.17817636397725	38.0	34.0	40.0	26.0	41.0
42	35.0648709455659	38.0	34.0	40.0	26.0	41.0
43	35.03609149428161	38.0	34.0	40.0	26.0	41.0
44	34.92903568526967	37.0	34.0	40.0	26.0	41.0
45	34.81123054809074	37.0	34.0	40.0	25.0	41.0
46	34.76117117680145	37.0	34.0	40.0	25.0	41.0
47	34.614274107868255	37.0	33.0	40.0	24.0	41.0
48	34.51571776763952	37.0	33.0	40.0	24.0	41.0
49	34.37807012061746	36.0	33.0	40.0	24.0	41.0
50	34.32966689581901	36.0	33.0	40.0	24.0	41.0
51	33.89219423786013	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	2.0
17	6.0
18	15.0
19	32.0
20	46.0
21	81.0
22	130.0
23	176.0
24	247.0
25	297.0
26	428.0
27	563.0
28	602.0
29	725.0
30	919.0
31	1055.0
32	1240.0
33	1629.0
34	2484.0
35	3287.0
36	2525.0
37	3134.0
38	4712.0
39	7663.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.08236985188426	23.37041434910318	30.62933566652084	13.91788013249172
2	29.7106430848072	27.62639835010312	29.570026873320415	13.092931691769264
3	29.3137928879445	26.67645772139241	30.319980001249924	13.689769389413161
4	26.932691706768324	27.773264170989314	30.463721017436413	14.83032310480595
5	23.08605712142991	33.094806574589086	29.41691144303481	14.402224860946191
6	21.47678270108118	41.98175114055371	26.457721392412974	10.083744765952128
7	88.81632397975126	3.9153802887319546	5.818386350853072	1.4499093806637084
8	89.2819198800075	3.4529091931754263	5.40903693519155	1.8561339916255235
9	83.95725267170802	6.968314480344978	6.462096118992563	2.6123367289544404
10	40.93494156615212	36.538341353665395	12.196112742953567	10.330604337228923
11	35.85713392912943	23.34854071620524	25.0734329104431	15.720892444222237
12	33.9478782576089	22.326729579401288	27.254546590838075	16.47084557215174
13	24.867195800262483	28.585713392912943	27.554527842009875	18.9925629648147
14	18.805074682832323	32.90731829260671	28.013874132866697	20.27373289169427
15	18.573839135054058	27.85450909318168	36.075870258108864	17.4957815136554
16	22.911068058246357	24.91406787075808	35.11030560589963	17.06455846509593
17	21.933004187238296	26.292106743328542	27.37328916942691	24.40159990000625
18	22.648584463471032	29.60127492031748	28.71070558090119	19.03943503531029
19	27.604524717205177	27.857633897881385	25.96087744515968	18.576963939753767
20	29.232547965752143	27.376413974126617	25.576526467095807	17.814511593025436
21	26.02649834385351	27.476407724517216	27.43891006812074	19.05818386350853
22	24.392225485907133	25.601524904693456	27.795137803887254	22.211111805512154
23	23.323542278607587	28.129491906755828	27.38891319292544	21.158052621711143
24	21.886132116742704	27.673270420598712	31.10118117617649	19.339416286482095
25	22.69233172926692	27.923254796575215	28.9638147615774	20.420598712580464
26	21.10805574651584	32.99793762889819	26.129616898943812	19.764389725642147
27	20.970564339728767	29.945003437285166	28.785700893694145	20.29873132929192
28	17.91138053871633	31.013686644584716	31.701143678520094	19.373789138178864
29	21.289294419098805	27.248296981438656	31.376163989750637	20.086244609711894
30	20.99556277732642	29.52627960752453	28.707580776201485	20.770576838947566
31	26.270233110430596	27.348290731829263	26.16398975064059	20.217486407099557
32	27.32954190363102	28.920067495781517	25.042184863446032	18.70820573714143
33	25.33279170051872	29.213799137553902	26.37647647022061	19.07693269170677
34	22.001749890631835	28.585713392912943	26.910818073870384	22.50171864258484
35	22.432972939191302	26.826448346978317	29.36691456783951	21.373664145990876
36	24.07349540653709	29.723142303606025	26.523342291106804	19.68001999875008
37	22.04862196112743	32.494844072245485	26.635835260296233	18.820698706330855
38	22.479845009686894	31.34179113805387	25.320292481719893	20.858071370539342
39	23.75789013186676	29.70126867070808	26.245234672832947	20.295606524592213
40	23.114180363727264	28.520092494219114	27.463908505718393	20.90181863633523
41	19.936253984125994	27.838885069683144	27.798262608586967	24.4265983376039
42	21.498656333979127	28.954440347478283	26.413974126617084	23.132929191925506
43	22.26423348540716	27.53265420911193	27.157677645147178	23.04543466033373
44	21.342416098993812	28.929441909880634	27.445159677520152	22.282982313605398
45	20.75807762014874	30.360602462346105	25.714017873882884	23.16730204362227
46	21.68926942066121	31.719892506718327	25.66402099868758	20.92681707393288
47	22.795450284357226	27.673270420598712	27.454534091619276	22.076745203424785
48	21.7361414911568	26.607712017998875	29.29191925504656	22.364227235797763
49	20.84244734704081	27.520154990313106	29.59190050621836	22.045497156427725
50	20.414349103181053	28.985688394475346	28.4638460096244	22.136116492719204
51	20.058121367414536	30.157490156865197	27.59515030310606	22.18923817261421
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	64.0
2	76.0
3	199.5
4	323.0
5	224.0
6	125.0
7	126.0
8	127.0
9	149.0
10	171.0
11	167.0
12	163.0
13	154.0
14	145.0
15	138.5
16	132.0
17	117.5
18	103.0
19	110.0
20	117.0
21	118.0
22	119.0
23	137.0
24	155.0
25	204.0
26	312.0
27	371.0
28	514.0
29	657.0
30	666.5
31	676.0
32	782.0
33	888.0
34	958.5
35	1029.0
36	1056.0
37	1083.0
38	1210.5
39	1338.0
40	1602.0
41	1866.0
42	2089.5
43	2313.0
44	2500.0
45	2687.0
46	2561.5
47	2436.0
48	2305.5
49	2175.0
50	1998.0
51	1821.0
52	1796.5
53	1772.0
54	1666.5
55	1561.0
56	1582.5
57	1604.0
58	1593.5
59	1583.0
60	1463.5
61	1344.0
62	1196.5
63	1049.0
64	844.0
65	639.0
66	541.0
67	443.0
68	340.0
69	237.0
70	194.0
71	151.0
72	121.5
73	92.0
74	81.0
75	49.5
76	29.0
77	22.5
78	16.0
79	10.5
80	5.0
81	3.5
82	2.0
83	2.5
84	3.0
85	1.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	32002.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.3776638960065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.9949896525433	37.86638335104056
2	15.886069055658425	18.23011061808637
3	8.697309661256943	14.97093931629273
4	4.623679337762772	10.611836760202488
5	2.559634026794467	7.343291044309732
6	1.078313909160222	3.712267983251047
7	0.5119268053588933	2.0561214924067244
8	0.26140943252369025	1.199925004687207
9	0.12525868641760157	0.6468345728391975
>10	0.2450713429909596	2.0217486407099554
>50	0.005446029844243546	0.19998750078120117
>100	0.010892059688487093	1.140553715392788
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	206	0.6437097681394913	No Hit
TGACTTGGGGTATATGAGTTCATATGCAGTGAGCTCATGAGTACCACAGCT	159	0.49684394725329667	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	64	0.19998750078120117	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	43	0.13436660208736956	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	40	0.12499218798825074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015624023498531342	0.0
2	0.0	0.0	0.0	0.04374726579588776	0.0
3	0.0	0.0	0.0	0.08436972689206924	0.0
4	0.0	0.0	0.0	0.20311230548090745	0.0
5	0.0	0.0	0.0	0.3343541028685707	0.0
6	0.0	0.0	0.0	0.5530904318480095	0.0
7	0.0	0.0	0.0	0.6562089869383163	0.0
8	0.0	0.0	0.0	0.8874445347165802	0.0
9	0.0	0.0	0.0	0.978063871008062	0.0
10	0.0	0.0	0.0	1.131179301293669	0.0
11	0.0	0.0	0.0	1.374914067870758	0.0
12	0.0	0.0	0.0	1.5499031310543092	0.0
13	0.0	0.0	0.0	1.6030248109493157	0.0
14	0.0	0.0	0.0	1.6248984438472596	0.0
15	0.0	0.0	0.0	1.678020123742266	0.0
16	0.0	0.0	0.0	1.72489219423786	0.0
17	0.0	0.0	0.0	1.8030123117305168	0.0
18	0.0	0.0	0.0	1.8998812574214112	0.0
19	0.0	0.0	0.0	1.9748765702143616	0.0
20	0.0	0.0	0.0	2.0561214924067244	0.0
21	0.0	0.0	0.0	2.1248671958002623	0.0
22	0.0	0.0	0.0	2.2092369226923316	0.0
23	0.0	0.0	0.0	2.2998562589838136	0.0
24	0.0	0.0	0.0	2.387350790575589	0.0
25	0.0	0.0	0.0	2.4623461033685397	0.0
26	0.0	0.0	0.0	2.549840634960315	0.0
27	0.0	0.0	0.0	2.6092119242547342	0.0
28	0.0	0.0	0.0	2.7154552840447472	0.0
29	0.0	0.0	0.0	2.79670020623711	0.0
30	0.0	0.0	0.0	2.9029435660271234	0.0
31	0.0	0.0	0.0	3.0123117305168425	0.0
32	0.0	0.0	0.0	3.124804699706268	0.0
33	0.0	0.0	0.0	3.202924817198925	0.0
34	0.0	0.0	0.0	3.331041809886882	0.0
35	0.0	0.0	0.0	3.459158802574839	0.0
36	0.0	0.0	0.0	3.552902943566027	0.0
37	0.0	0.0	0.0	3.6310230610586838	0.0
38	0.0	0.0	0.0	3.7372664208486968	0.0
39	0.0	0.0	0.0	3.821636147740766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGAT	45	3.601599E-10	45.000004	5
GGATGTA	25	3.80617E-5	45.0	8
TAGCGGG	20	6.9308653E-4	45.0	3
GCGGGAC	20	6.9308653E-4	45.0	5
ATGGGAT	40	6.4646883E-9	45.0	5
GTATGGG	20	6.9308653E-4	45.0	3
CAATGGG	25	3.80617E-5	45.0	3
GGGTATA	20	6.9308653E-4	45.0	8
TATGAGT	20	6.9308653E-4	45.0	13
GTGAGCT	20	6.9308653E-4	45.0	29
TTGGGAT	35	1.16318915E-7	45.0	5
ACGGGGG	20	6.9308653E-4	45.0	5
ACGGGAT	20	6.9308653E-4	45.0	5
AGTGAGC	20	6.9308653E-4	45.0	28
GGGATAT	25	3.80617E-5	45.0	7
ACTAGGG	20	6.9308653E-4	45.0	3
GGGAATA	20	6.9308653E-4	45.0	7
AACAGGG	25	3.80617E-5	45.0	3
TGAGCTC	20	6.9308653E-4	45.0	30
GGTATAT	20	6.9308653E-4	45.0	9
>>END_MODULE