FastQCFastQC Report
Thu 26 May 2016
SRR1547327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547327_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences448606
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61541.3718051029188196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG207.028682E-445.0000041
CGAAAGG406.7993824E-945.0000042
TACGGCG302.162531E-645.00000415
TAACGCC351.2097189E-745.00000412
AATCGAG207.028682E-445.0000041
GTACCGG253.8869897E-545.02
CGTTTTT32150.043.3903581
GGCGATA850.042.3529438
GCGAGAC750.042.00000421
TTAACGG650.041.5384642
TCGGGAT556.002665E-1140.9090925
CCGTTAC451.9237632E-840.00000445
ACGGGAT2200.039.8863645
TACGGGA1750.039.8571434
GGGCGAT3850.039.7402577
ACACGGG850.039.7058833
GTTAACG403.4519326E-739.3750041
ATGGTCG403.4519326E-739.37500444
ATAGGGA7050.038.9361724
TATGGGA5800.038.7931024
Produced by FastQC (version 0.11.5)