##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547327_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	448606
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294813265983958	31.0	31.0	33.0	30.0	34.0
2	31.718804474304847	31.0	31.0	34.0	30.0	34.0
3	31.762941200073115	31.0	31.0	34.0	30.0	34.0
4	35.57902480127328	37.0	35.0	37.0	33.0	37.0
5	35.486598485084905	37.0	35.0	37.0	33.0	37.0
6	35.52254762531041	37.0	35.0	37.0	33.0	37.0
7	35.899615698407956	37.0	35.0	37.0	35.0	37.0
8	35.87597134233604	37.0	35.0	37.0	35.0	37.0
9	37.569428407110024	39.0	37.0	39.0	35.0	39.0
10	36.95349816988627	39.0	37.0	39.0	32.0	39.0
11	36.7089472722166	39.0	37.0	39.0	32.0	39.0
12	36.456199872493904	39.0	35.0	39.0	32.0	39.0
13	36.37319607851879	39.0	35.0	39.0	32.0	39.0
14	37.4822204785491	40.0	36.0	41.0	32.0	41.0
15	37.58983606995894	40.0	36.0	41.0	32.0	41.0
16	37.611068510006554	40.0	36.0	41.0	32.0	41.0
17	37.51288881557536	39.0	36.0	41.0	32.0	41.0
18	37.351468772151954	39.0	36.0	41.0	32.0	41.0
19	37.252404114077834	39.0	36.0	41.0	32.0	41.0
20	37.05086423275658	39.0	35.0	41.0	32.0	41.0
21	36.92376160818179	39.0	35.0	41.0	31.0	41.0
22	36.85064176582569	39.0	35.0	41.0	31.0	41.0
23	36.76280076503658	38.0	35.0	40.0	31.0	41.0
24	36.62848245453694	38.0	35.0	40.0	31.0	41.0
25	36.540527768242065	38.0	35.0	40.0	31.0	41.0
26	36.41221472739999	38.0	35.0	40.0	30.0	41.0
27	36.32938703450244	38.0	35.0	40.0	30.0	41.0
28	36.36576193809267	38.0	35.0	40.0	30.0	41.0
29	36.351040779659655	38.0	35.0	40.0	30.0	41.0
30	36.26285426409812	38.0	35.0	40.0	30.0	41.0
31	36.12498272426138	38.0	35.0	40.0	30.0	41.0
32	35.967989728180186	38.0	35.0	40.0	29.0	41.0
33	35.69704373102455	38.0	35.0	40.0	27.0	41.0
34	35.390964454331865	38.0	34.0	40.0	25.0	41.0
35	35.159193144986915	38.0	34.0	40.0	24.0	41.0
36	34.995967508236625	38.0	34.0	40.0	24.0	41.0
37	34.8787332313879	38.0	34.0	40.0	23.0	41.0
38	34.774940593750415	38.0	34.0	40.0	23.0	41.0
39	34.7204629452125	38.0	34.0	40.0	23.0	41.0
40	34.541914285586905	38.0	33.0	40.0	23.0	41.0
41	34.452940887995254	38.0	33.0	40.0	22.0	41.0
42	34.390587731773536	38.0	33.0	40.0	22.0	41.0
43	34.3181834393655	38.0	33.0	40.0	22.0	41.0
44	34.19612087221304	37.0	33.0	40.0	21.0	41.0
45	34.085308711876344	37.0	33.0	40.0	21.0	41.0
46	34.00361118665377	37.0	33.0	40.0	21.0	41.0
47	33.86135495289854	37.0	33.0	40.0	20.0	41.0
48	33.800381626638966	37.0	33.0	40.0	20.0	41.0
49	33.67997307213903	37.0	33.0	40.0	20.0	41.0
50	33.6202123912743	36.0	33.0	40.0	20.0	41.0
51	33.183847295845354	36.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	9.0
15	18.0
16	44.0
17	148.0
18	253.0
19	510.0
20	817.0
21	1282.0
22	2023.0
23	2917.0
24	4217.0
25	6280.0
26	8437.0
27	10139.0
28	10631.0
29	10943.0
30	12356.0
31	14315.0
32	16897.0
33	21375.0
34	30571.0
35	37621.0
36	37063.0
37	46172.0
38	67188.0
39	106344.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.53992590379977	23.394693784746526	27.06651270825624	17.99886760319746
2	34.66850643994953	24.849868258560964	27.878360967084703	12.603264334404802
3	28.35026727239493	25.16060864099009	33.68590700971454	12.803217076900442
4	25.155258734836362	27.357413855365287	33.13330628658556	14.354021123212796
5	22.66799819886493	30.996241690927008	32.321458027757096	14.01430208245097
6	21.033378956144144	39.56099561753521	29.64806534018716	9.75756008613349
7	85.5262747265975	3.1653611409566524	9.917165619719755	1.3911985127260893
8	85.98569791754903	2.7083899903255864	9.893536867540782	1.412375224584602
9	80.89281017195489	5.780796511861188	10.98246568258115	2.343927633602761
10	33.933117256568124	38.92658591280544	17.078683744755978	10.061613085870452
11	27.71429717837033	24.154603371332527	30.929367864005386	17.201731586291757
12	26.105758728148977	22.347449655153966	33.450956964463245	18.09583465223381
13	23.56410747961463	24.054961369219317	33.703962942983374	18.676968208182682
14	19.391849417974793	27.708947272216598	33.48818339478295	19.41101991502566
15	19.518686776369464	27.406900487287285	35.78106400716887	17.293348729174376
16	24.01550581133556	26.44168825205191	33.390770520233794	16.152035416378737
17	22.89224843180876	26.02283518276617	31.820573064114164	19.264343321310907
18	22.864830162770893	27.029286277936542	32.290918980129554	17.814964579163007
19	23.94528829306786	28.007650365799837	30.42045804113186	17.626603300000447
20	25.558507911173724	27.97153849926216	30.1783747876756	16.29157880188852
21	25.665060208735508	25.50545467514924	32.744323526658135	16.085161589457115
22	23.7651747858923	22.89603794866765	33.69950468785527	19.639282577584787
23	21.52601614780007	26.111554459815515	33.02296447216489	19.339464920219523
24	20.612296759294345	27.208062308573673	33.67498428465067	18.504656647481312
25	22.103806012402867	28.233015162525692	31.05977182650254	18.6034069985689
26	21.14193746851357	29.712041301275505	29.58721015768848	19.558811072522435
27	19.77815722482535	28.377462628676387	32.83281989095108	19.011560255547185
28	19.211735910799234	27.93654119650651	33.97836854611842	18.873354346575837
29	20.283946269109197	27.277611088572158	33.355104479208926	19.083338163109723
30	21.258743752870004	26.378158116476374	33.529868080230756	18.833230050422866
31	22.678252185659574	27.12045759530635	31.3056445968177	18.895645622216378
32	23.07682019411243	27.264459235944237	31.11906661970638	18.539653950236957
33	22.43772932149815	27.247072040944616	30.253719299340627	20.06147933821661
34	20.703468076664155	28.33354881566453	31.317013147394373	19.645969960276947
35	20.891829355826715	28.138500153809805	30.97729410663255	19.992376383730935
36	22.944632929564026	28.90264508276751	29.457920758973355	18.694801228695113
37	21.648841076579448	29.5796311239707	29.164344658787446	19.607183140662407
38	21.80242796574277	30.109271833189926	28.169930852462965	19.918369348604344
39	21.464046401519372	28.70804224642559	28.272024894896635	21.555886457158397
40	22.140586617209756	28.163466382527208	29.74191161063383	19.954035389629208
41	20.62856939051194	27.941222364391027	29.77066735621013	21.65954088888691
42	21.329852922163322	29.036615649367153	28.81949862462829	20.814032803841233
43	21.176043120243598	28.3571775678435	29.22408527750409	21.242694034408814
44	21.127448139347223	28.66658047373419	29.488905632113706	20.717065754804885
45	21.383129070944214	28.87299768616559	28.69266126623362	21.051211976656575
46	21.79618640856342	28.699794474438594	28.90153051898548	20.60248859801251
47	21.310905337868867	28.473538026687116	29.902408795245716	20.313147840198305
48	21.343227687547646	28.470863073610253	29.834420404542072	20.35148883430003
49	21.296416008702515	28.012777359197155	30.36205489895365	20.32875173314668
50	20.252515570456033	28.779151415718918	30.50382741202748	20.46450560179757
51	20.32652260558263	29.02792205186734	29.657427675956182	20.988127666593847
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	577.0
1	700.0
2	823.0
3	5255.5
4	9688.0
5	6770.5
6	3853.0
7	3826.0
8	3799.0
9	3868.0
10	3937.0
11	3980.5
12	4024.0
13	3964.5
14	3905.0
15	3663.0
16	3421.0
17	3171.5
18	2922.0
19	2902.0
20	2882.0
21	2978.5
22	3075.0
23	3218.5
24	3362.0
25	3993.5
26	5320.0
27	6015.0
28	7040.0
29	8065.0
30	9080.5
31	10096.0
32	11252.0
33	12408.0
34	13803.5
35	15199.0
36	16681.5
37	18164.0
38	19888.0
39	21612.0
40	22635.5
41	23659.0
42	25714.0
43	27769.0
44	28902.5
45	30036.0
46	30698.5
47	31361.0
48	31225.0
49	31089.0
50	30169.0
51	29249.0
52	27460.0
53	25671.0
54	23685.5
55	21700.0
56	21017.5
57	20335.0
58	18727.0
59	17119.0
60	15463.0
61	13807.0
62	12171.0
63	10535.0
64	9256.5
65	7978.0
66	6752.0
67	5526.0
68	4625.5
69	3725.0
70	3257.5
71	2790.0
72	2291.0
73	1792.0
74	1333.0
75	738.5
76	603.0
77	457.0
78	311.0
79	219.5
80	128.0
81	84.0
82	40.0
83	34.5
84	29.0
85	22.0
86	15.0
87	10.5
88	6.0
89	4.0
90	2.0
91	2.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	448606.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.46358232843341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.8241250567642	24.40853064733057
2	6.860971377071194	4.7290730381343655
3	3.77217544013697	3.9000803651536504
4	2.730981155766666	3.7647757559665886
5	2.175461817109799	3.748710371816345
6	1.7798322838605705	3.6803637867379453
7	1.5411750522746441	3.7180089307616404
8	1.3753009054280256	3.7918236784470225
9	1.1268516266489428	3.495180942625046
>10	7.772250672989796	42.03932294254126
>50	0.030493758120865237	0.7466033436494518
>100	0.009732050464105926	0.6014863714935685
>500	0.0	0.0
>1k	0.0	0.0
>5k	6.488033642737284E-4	1.3760398253425354
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6154	1.3718051029188196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014489329166350873	0.0
2	0.0	0.0	0.0	0.04458255128107961	0.0
3	0.0	0.0	0.0	0.08604432397248366	0.0
4	0.0	0.0	0.0	0.13998921102259	0.0
5	0.0	0.0	0.0	0.25478928057136996	0.0
6	0.0	0.0	0.0	0.5046744805018212	0.0
7	0.0	0.0	0.0	0.6502365104345461	0.0
8	0.0	0.0	0.0	0.856207897353134	0.0
9	0.0	0.0	0.0	0.9955283701065077	0.0
10	0.0	0.0	0.0	1.141313312795638	0.0
11	0.0	0.0	0.0	1.2993584570870653	0.0
12	0.0	0.0	0.0	1.4221833858664397	0.0
13	0.0	0.0	0.0	1.4950758572110048	0.0
14	0.0	0.0	0.0	1.5369834554152195	0.0
15	0.0	0.0	0.0	1.5650704627222998	0.0
16	0.0	0.0	0.0	1.615002920157109	0.0
17	0.0	0.0	0.0	1.669393632720026	0.0
18	0.0	0.0	0.0	1.7465214464362937	0.0
19	0.0	0.0	0.0	1.8020267227812379	0.0
20	0.0	0.0	0.0	1.859761126690236	0.0
21	0.0	0.0	0.0	1.9270807791246662	0.0
22	0.0	0.0	0.0	2.006660633161393	0.0
23	0.0	0.0	0.0	2.088246702005769	0.0
24	0.0	0.0	0.0	2.157795482004253	0.0
25	0.0	0.0	0.0	2.2271213492463318	0.0
26	0.0	0.0	0.0	2.287084880719384	0.0
27	0.0	0.0	0.0	2.353067056615382	0.0
28	0.0	0.0	0.0	2.4152597156524878	0.0
29	0.0	0.0	0.0	2.5013040396249715	0.0
30	0.0	0.0	0.0	2.5871254508410497	0.0
31	0.0	0.0	0.0	2.66559074109575	0.0
32	0.0	0.0	0.0	2.7467309844273147	0.0
33	0.0	0.0	0.0	2.826979576733258	0.0
34	0.0	0.0	0.0	2.9139155517313635	0.0
35	0.0	0.0	0.0	3.0131117283317654	0.0
36	0.0	0.0	0.0	3.1004935288426814	0.0
37	0.0	0.0	0.0	3.2037021350583808	0.0
38	0.0	0.0	0.0	3.3320998827478903	0.0
39	0.0	0.0	0.0	3.5478794309483153	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	20	7.028682E-4	45.000004	1
CGAAAGG	40	6.7993824E-9	45.000004	2
TACGGCG	30	2.162531E-6	45.000004	15
TAACGCC	35	1.2097189E-7	45.000004	12
AATCGAG	20	7.028682E-4	45.000004	1
GTACCGG	25	3.8869897E-5	45.0	2
CGTTTTT	3215	0.0	43.390358	1
GGCGATA	85	0.0	42.352943	8
GCGAGAC	75	0.0	42.000004	21
TTAACGG	65	0.0	41.538464	2
TCGGGAT	55	6.002665E-11	40.909092	5
CCGTTAC	45	1.9237632E-8	40.000004	45
ACGGGAT	220	0.0	39.886364	5
TACGGGA	175	0.0	39.857143	4
GGGCGAT	385	0.0	39.740257	7
ACACGGG	85	0.0	39.705883	3
GTTAACG	40	3.4519326E-7	39.375004	1
ATGGTCG	40	3.4519326E-7	39.375004	44
ATAGGGA	705	0.0	38.936172	4
TATGGGA	580	0.0	38.793102	4
>>END_MODULE