FastQCFastQC Report
Thu 26 May 2016
SRR1547326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547326_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences204201
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32341.5837336741739756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA207.019651E-445.024
CGTATGG207.019651E-445.02
GTTAGCG207.019651E-445.01
TAGCTAG406.7684596E-945.01
AGTACGG207.019651E-445.02
GTTACGG302.1567103E-644.9999962
TTGCACG302.1567103E-644.9999961
TACGTTG302.1567103E-644.9999961
CGTTTTT18550.043.423181
TTAGGGA2150.041.8604664
TAGGGAT2800.040.982145
GTTGATC556.002665E-1140.90909216
TGCGAGG556.002665E-1140.9090922
AAGGGCG556.002665E-1140.9090925
GAGGGTA1100.040.9090925
CGTGGGA501.0713848E-940.54
TTAGCGG501.0713848E-940.52
TAGGGCG501.0713848E-940.55
TAGGGAC1850.040.1351365
GTAGGGA2900.039.568974
Produced by FastQC (version 0.11.5)