FastQCFastQC Report
Thu 26 May 2016
SRR1547325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547325_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3862931
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT936312.42383309461132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57600.14910957508689646No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53740.13911716258975373No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40020.10360009019058326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT430750.043.7986071
CGGTCTA6600.041.59090831
CGACGGT6550.041.22137528
CGGTCGA403.4610457E-739.37500418
GCCGATA1450.038.7931069
CGTTAGT356.252123E-638.57142615
TACGGGA13850.038.5018044
AACGGGC4750.038.368424
TAGGGAC34050.038.0616765
TAGGGAT50700.037.9437875
GTTTTTT507300.037.930222
ATAGGGA44400.037.5506744
CGTAAGG4800.037.5000042
TTAGGGA43850.037.405934
AGGGCGA20350.037.371016
TAGGGTA24950.037.334675
TACGGGC4900.037.193884
TAAGGGA44350.037.1364174
GCGTAAG3100.037.016131
AGGGATC31850.036.875986
Produced by FastQC (version 0.11.5)