##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547325_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3862931
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.365567233792163	31.0	31.0	33.0	30.0	34.0
2	31.76709239693901	31.0	31.0	34.0	30.0	34.0
3	31.71799832821244	31.0	31.0	34.0	30.0	34.0
4	35.57517568913346	37.0	35.0	37.0	33.0	37.0
5	35.50888923462521	37.0	35.0	37.0	33.0	37.0
6	35.56310117887169	37.0	35.0	37.0	33.0	37.0
7	35.95925192554565	37.0	35.0	37.0	35.0	37.0
8	35.947104413721085	37.0	35.0	37.0	35.0	37.0
9	37.671738376895675	39.0	37.0	39.0	35.0	39.0
10	37.01664073212801	39.0	37.0	39.0	33.0	39.0
11	36.78495111613435	39.0	37.0	39.0	32.0	39.0
12	36.534564298456274	39.0	35.0	39.0	32.0	39.0
13	36.432683110311835	39.0	35.0	39.0	32.0	39.0
14	37.57924695004907	40.0	36.0	41.0	32.0	41.0
15	37.666616100572334	40.0	36.0	41.0	32.0	41.0
16	37.70027396295714	40.0	36.0	41.0	32.0	41.0
17	37.59889343092072	40.0	36.0	41.0	32.0	41.0
18	37.35700145821916	39.0	36.0	41.0	32.0	41.0
19	37.18807299431442	38.0	36.0	41.0	32.0	41.0
20	36.923138155975344	38.0	35.0	41.0	32.0	41.0
21	36.80973540557675	38.0	35.0	40.0	31.0	41.0
22	36.72687914953697	38.0	35.0	40.0	31.0	41.0
23	36.64558259000743	38.0	35.0	40.0	31.0	41.0
24	36.49408208430334	38.0	35.0	40.0	31.0	41.0
25	36.400346524439605	38.0	35.0	40.0	31.0	41.0
26	36.259886599061694	38.0	35.0	40.0	30.0	41.0
27	36.15456294715075	38.0	35.0	40.0	30.0	41.0
28	36.17392259918699	38.0	35.0	40.0	30.0	41.0
29	36.12711668937395	38.0	35.0	40.0	30.0	41.0
30	36.0171499309721	38.0	35.0	40.0	30.0	41.0
31	35.824548251056	38.0	35.0	40.0	29.0	41.0
32	35.57951850550786	38.0	35.0	40.0	28.0	41.0
33	35.19261384684324	38.0	34.0	40.0	25.0	41.0
34	34.74629419992229	38.0	34.0	40.0	23.0	41.0
35	34.43595756693557	38.0	33.0	40.0	21.0	41.0
36	34.23610491618929	38.0	33.0	40.0	20.0	41.0
37	34.124854676410216	38.0	33.0	40.0	20.0	41.0
38	34.010800865974566	38.0	33.0	40.0	18.0	41.0
39	33.95017047935881	37.0	33.0	40.0	18.0	41.0
40	33.795948982780175	37.0	33.0	40.0	18.0	41.0
41	33.707498011225155	37.0	33.0	40.0	18.0	41.0
42	33.61977912626449	37.0	33.0	40.0	18.0	41.0
43	33.559371109657405	37.0	33.0	40.0	18.0	41.0
44	33.421854804033515	37.0	33.0	40.0	17.0	41.0
45	33.31124941139254	36.0	33.0	40.0	17.0	41.0
46	33.225917056245635	36.0	32.0	40.0	17.0	41.0
47	33.09549277478681	36.0	32.0	40.0	17.0	41.0
48	33.00706613708606	36.0	32.0	40.0	15.0	41.0
49	32.886377986042206	36.0	32.0	40.0	15.0	41.0
50	32.80825932433171	35.0	31.0	40.0	15.0	41.0
51	32.39206395351095	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	10.0
12	9.0
13	34.0
14	63.0
15	200.0
16	497.0
17	1042.0
18	2356.0
19	4501.0
20	7770.0
21	12433.0
22	19280.0
23	28880.0
24	43692.0
25	67016.0
26	93423.0
27	103923.0
28	101847.0
29	99937.0
30	107172.0
31	122848.0
32	146532.0
33	183531.0
34	268929.0
35	354382.0
36	329302.0
37	381956.0
38	539631.0
39	841469.0
40	260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.284361796780736	22.033839071937862	26.57337135972659	21.108427771554812
2	37.30346206028531	23.82509550390623	26.81761595016841	12.053826485640048
3	26.759421796558108	23.105408820400882	37.58604541473819	12.549123968302824
4	24.11945747930781	25.624920559026293	36.638837193830284	13.616784767835616
5	21.2190432601566	29.63889336879173	35.91128083830646	13.230782532745215
6	19.610937912170836	37.93849799543404	32.91130491328993	9.539259179105192
7	80.69691123139398	3.348105363518013	14.327229764135058	1.6277536409529447
8	81.1859699280158	2.8647418242779903	14.1280028040884	1.8212854436178125
9	76.4939109707111	5.489329216597449	15.393906854665538	2.6228529580259137
10	34.94892867617879	30.26895898477089	22.84022158304148	11.941890756008844
11	27.34291655740162	24.69536732600194	31.213112530355836	16.748603586240606
12	25.888114491302073	21.686745116596697	34.74092599634837	17.68421439575286
13	22.05532534751462	24.335355718235714	35.68197309245234	17.92734584179733
14	18.653607843370747	28.189320492651827	35.58150533882174	17.57556632515569
15	18.185828325693628	27.219305755137746	37.87139868664494	16.7234672325237
16	21.30449650796248	25.228977685596764	37.00568299045466	16.460842815986098
17	21.32844205604501	25.395017410354985	33.60378427675773	19.672756256842277
18	21.041613220634797	27.762856753071695	34.48117504558068	16.71435498071283
19	22.97840681078694	28.670975484677307	31.547004075402846	16.803613629132904
20	23.276729509276763	27.66932673661528	32.40127768267153	16.652666071436432
21	23.667909160168794	26.316312665175744	33.95494250350317	16.060835671152294
22	22.168193011990116	24.40266212365688	33.93143703576378	19.497707828589224
23	20.43010864030447	26.872807202613767	33.883494165440695	18.81358999164106
24	20.238570142723233	27.356766144670974	35.12011992965963	17.284543782946162
25	21.07135747441515	28.00565166708906	33.06792174128919	17.855069117206597
26	19.450179151530275	29.49788644943438	32.1048706280283	18.94706377100704
27	18.40586849726283	29.274066764329987	34.49595656769432	17.82410817071286
28	18.048109065370312	28.998291711656254	35.429729394597004	17.52386982837643
29	19.0410597548856	27.5046849141235	34.963891407845495	18.490363923145402
30	19.51015951359214	27.532332314504192	35.27937723971772	17.678130932185947
31	21.499426212893784	27.77802658137047	32.584635863286195	18.137911342449556
32	21.834845095602276	28.416117191842154	32.443214750664715	17.305822961890854
33	21.318164885678776	28.13350795031027	31.16038055041625	19.387946613594703
34	20.060622361621267	29.105412444591945	32.23381416856786	18.600151025218935
35	20.268573267293668	28.7682332405109	31.559947614906918	19.403245877288512
36	22.42983371952541	30.067039768507385	29.516991113742392	17.98613539822482
37	20.32386806805506	31.131102264058043	29.930640749213488	18.61438891867341
38	20.53407114960117	31.53641626008852	28.67431491786936	19.255197672440953
39	20.336216204741945	29.95375791076776	28.986047123285402	20.723978761204897
40	21.172990146601116	28.798495235871414	30.14829930951394	19.880215308013526
41	19.25077098193056	28.256135043571838	31.05867021699326	21.43442375750434
42	20.101368623980083	29.99662691360524	29.25452201967884	20.64748244273584
43	19.774932557687414	29.053353528706573	30.08117411364583	21.090539799960187
44	19.70198276904247	29.786501493296154	29.819818163979633	20.691697573681747
45	19.98829386287252	30.458012322767345	29.1936614969307	20.360032317429432
46	20.7519626936127	30.393294625246998	29.285379417856543	19.56936326328376
47	20.44571854894638	29.584375180400578	29.882982636759497	20.086923633893537
48	19.988371524109542	29.8200511476907	30.357026827556588	19.834550500643164
49	20.223607411056527	28.946569327797985	30.330207813704153	20.499615447441332
50	19.513964914206337	29.21359454776697	31.01220291017365	20.260237627853048
51	18.764326880288568	30.163184379943626	30.409551710864108	20.6629370289037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6972.0
1	7773.5
2	8575.0
3	71226.0
4	133877.0
5	89118.5
6	44360.0
7	44842.5
8	45325.0
9	48467.0
10	51609.0
11	52785.0
12	53961.0
13	52790.0
14	51619.0
15	49823.5
16	48028.0
17	43548.5
18	39069.0
19	36234.0
20	33399.0
21	33737.5
22	34076.0
23	33679.0
24	33282.0
25	36303.5
26	44816.0
27	50307.0
28	57857.0
29	65407.0
30	70896.0
31	76385.0
32	87656.5
33	98928.0
34	108062.5
35	117197.0
36	126758.5
37	136320.0
38	149754.0
39	163188.0
40	176333.0
41	189478.0
42	210173.0
43	230868.0
44	255003.0
45	279138.0
46	276162.0
47	273186.0
48	268633.5
49	264081.0
50	243266.0
51	222451.0
52	208950.0
53	195449.0
54	184333.5
55	173218.0
56	165407.0
57	157596.0
58	148264.0
59	138932.0
60	129599.5
61	120267.0
62	110500.0
63	100733.0
64	83257.5
65	65782.0
66	54161.5
67	42541.0
68	34917.0
69	27293.0
70	23317.5
71	19342.0
72	15997.0
73	12652.0
74	10620.5
75	6723.0
76	4857.0
77	3711.5
78	2566.0
79	1884.5
80	1203.0
81	949.5
82	696.0
83	585.0
84	474.0
85	325.0
86	176.0
87	119.5
88	63.0
89	56.5
90	50.0
91	36.0
92	22.0
93	16.5
94	11.0
95	8.5
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3862931.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.371303623038354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.55083536597975	18.825780304276883
2	7.273982634383117	3.4000491339375243
3	2.680926819801461	1.8797026409017974
4	1.4458250591481516	1.3516326577259536
5	0.9582256582755395	1.1197491399471715
6	0.7172827267722788	1.0058299434573474
7	0.5553584255963063	0.9085615268956682
8	0.4423546373585315	0.8270723631012212
9	0.36679295101198484	0.7715186482402198
>10	3.2591859383799235	16.58751678984386
>50	0.8784806467616915	14.84273409986591
>100	0.8617295182391593	33.57445217122172
>500	0.005186280517902306	0.8070223598758592
>1k	0.0034951020881515324	1.337817358319687
>5k	2.2549045730009887E-4	0.29338123077212186
>10k+	1.1274522865004943E-4	2.467179631617077
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93631	2.42383309461132	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5760	0.14910957508689646	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	5374	0.13911716258975373	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	4002	0.10360009019058326	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0354831603256698E-4	0.0	0.0	0.03536174992512162	0.0
2	1.0354831603256698E-4	0.0	0.0	0.0912778405827078	0.0
3	1.0354831603256698E-4	0.0	0.0	0.15159473467167806	0.0
4	1.0354831603256698E-4	0.0	0.0	0.25405579338590306	0.0
5	1.0354831603256698E-4	0.0	0.0	0.4860816825358775	0.0
6	1.0354831603256698E-4	0.0	0.0	0.958805632303554	0.0
7	1.0354831603256698E-4	0.0	0.0	1.2669654208164733	0.0
8	1.0354831603256698E-4	0.0	0.0	1.6580674104714788	0.0
9	1.0354831603256698E-4	0.0	0.0	1.8778745983296103	0.0
10	1.0354831603256698E-4	0.0	0.0	2.1508797335494734	0.0
11	1.0354831603256698E-4	0.0	0.0	2.4391582453841396	0.0
12	1.0354831603256698E-4	0.0	0.0	2.6595349489804505	0.0
13	1.0354831603256698E-4	0.0	0.0	2.7919473581070955	0.0
14	1.0354831603256698E-4	0.0	0.0	2.863473357406591	0.0
15	1.0354831603256698E-4	0.0	0.0	2.9268967009765383	0.0
16	1.0354831603256698E-4	0.0	0.0	3.0326971928828135	0.0
17	1.0354831603256698E-4	0.0	0.0	3.15226961082142	0.0
18	1.0354831603256698E-4	0.0	0.0	3.303812571335082	0.0
19	1.2943539504070873E-4	0.0	0.0	3.4017692783018902	0.0
20	1.2943539504070873E-4	0.0	0.0	3.502133483616456	0.0
21	1.2943539504070873E-4	0.0	0.0	3.6304557342598147	0.0
22	1.2943539504070873E-4	0.0	0.0	3.7750350705202864	0.0
23	1.2943539504070873E-4	0.0	0.0	3.937321168822327	0.0
24	1.2943539504070873E-4	0.0	0.0	4.069863013344013	0.0
25	1.2943539504070873E-4	0.0	0.0	4.193007848185743	0.0
26	1.2943539504070873E-4	0.0	0.0	4.309422042485356	0.0
27	1.2943539504070873E-4	0.0	0.0	4.43349881217138	0.0
28	1.2943539504070873E-4	0.0	0.0	4.569509525280156	0.0
29	1.2943539504070873E-4	0.0	0.0	4.70779830134165	0.0
30	1.2943539504070873E-4	0.0	0.0	4.867728675453949	0.0
31	1.2943539504070873E-4	0.0	0.0	5.019323410125628	0.0
32	1.5532247404885047E-4	0.0	0.0	5.173066772354981	0.0
33	1.5532247404885047E-4	0.0	0.0	5.326836021663343	0.0
34	1.812095530569922E-4	0.0	0.0	5.484643655296975	0.0
35	1.812095530569922E-4	0.0	0.0	5.655110070565589	0.0
36	1.812095530569922E-4	0.0	0.0	5.817784475052751	0.0
37	1.812095530569922E-4	0.0	0.0	5.989830002140861	0.0
38	1.812095530569922E-4	0.0	0.0	6.179944710376654	0.0
39	1.812095530569922E-4	0.0	0.0	6.4031172185058445	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	43075	0.0	43.798607	1
CGGTCTA	660	0.0	41.590908	31
CGACGGT	655	0.0	41.221375	28
CGGTCGA	40	3.4610457E-7	39.375004	18
GCCGATA	145	0.0	38.793106	9
CGTTAGT	35	6.252123E-6	38.571426	15
TACGGGA	1385	0.0	38.501804	4
AACGGGC	475	0.0	38.36842	4
TAGGGAC	3405	0.0	38.061676	5
TAGGGAT	5070	0.0	37.943787	5
GTTTTTT	50730	0.0	37.93022	2
ATAGGGA	4440	0.0	37.550674	4
CGTAAGG	480	0.0	37.500004	2
TTAGGGA	4385	0.0	37.40593	4
AGGGCGA	2035	0.0	37.37101	6
TAGGGTA	2495	0.0	37.33467	5
TACGGGC	490	0.0	37.19388	4
TAAGGGA	4435	0.0	37.136417	4
GCGTAAG	310	0.0	37.01613	1
AGGGATC	3185	0.0	36.87598	6
>>END_MODULE