FastQCFastQC Report
Thu 26 May 2016
SRR1547324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547324_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1886399
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165680.8782871492192267No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21100.11185332477381509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTTA302.1662454E-645.00000440
ACGATAA253.8917606E-545.010
CCGTAGA207.034439E-445.019
TCGAGTA453.8562575E-1045.027
CGCGTAT207.034439E-445.026
CGACTAG207.034439E-445.026
TAGTACG551.8189894E-1245.01
ACGCGTA207.034439E-445.025
TATAGCG1300.044.9999961
TACGCGG1650.043.6363642
ATATGCG1000.042.751
CGTTTTT74400.042.3084681
CGGTCTA4100.041.70731731
GGCGATA5600.041.3839268
TAACGCC2400.041.25000412
ATAACGC2400.041.25000411
TCTCACG4450.040.95505523
CGGGTTA1100.040.9090926
CGAGTAC501.0822987E-940.528
ATATCGG501.0822987E-940.52