##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547324_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1886399
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.296078401229007	31.0	31.0	33.0	30.0	34.0
2	31.721622519944084	31.0	31.0	34.0	30.0	34.0
3	31.846754583733347	31.0	31.0	34.0	30.0	34.0
4	35.622194986320494	37.0	35.0	37.0	33.0	37.0
5	35.49820425053236	37.0	35.0	37.0	33.0	37.0
6	35.53065814814363	37.0	35.0	37.0	33.0	37.0
7	35.90576967014932	37.0	35.0	37.0	35.0	37.0
8	35.8892805816797	37.0	35.0	37.0	35.0	37.0
9	37.614028103280376	39.0	37.0	39.0	35.0	39.0
10	36.91399221479655	39.0	37.0	39.0	32.0	39.0
11	36.65321917579473	39.0	35.0	39.0	32.0	39.0
12	36.265790005189785	38.0	35.0	39.0	32.0	39.0
13	36.06592613757747	38.0	35.0	39.0	31.0	39.0
14	37.094361797265584	39.0	35.0	41.0	31.0	41.0
15	37.261941402640694	39.0	35.0	41.0	32.0	41.0
16	37.35518996776398	39.0	35.0	41.0	32.0	41.0
17	37.28654595342767	39.0	35.0	41.0	32.0	41.0
18	37.165128374219876	39.0	35.0	41.0	32.0	41.0
19	37.10534409740463	39.0	35.0	41.0	32.0	41.0
20	36.94486479265521	39.0	35.0	41.0	31.0	41.0
21	36.804867368992454	38.0	35.0	41.0	31.0	41.0
22	36.74764564654667	38.0	35.0	40.0	31.0	41.0
23	36.68655676768277	38.0	35.0	40.0	31.0	41.0
24	36.53562793449318	38.0	35.0	40.0	31.0	41.0
25	36.45458887541819	38.0	35.0	40.0	31.0	41.0
26	36.34043328055199	38.0	35.0	40.0	30.0	41.0
27	36.257891358084905	38.0	35.0	40.0	30.0	41.0
28	36.32912337209678	38.0	35.0	40.0	30.0	41.0
29	36.33510089859038	38.0	35.0	40.0	30.0	41.0
30	36.27113776035717	38.0	35.0	40.0	30.0	41.0
31	36.15840763274365	38.0	35.0	40.0	30.0	41.0
32	36.06955898513517	38.0	35.0	40.0	30.0	41.0
33	35.882374831623636	38.0	35.0	40.0	29.0	41.0
34	35.65395072834538	38.0	34.0	40.0	28.0	41.0
35	35.51467266469077	38.0	34.0	40.0	27.0	41.0
36	35.3752853982641	38.0	34.0	40.0	27.0	41.0
37	35.27002081744106	38.0	34.0	40.0	26.0	41.0
38	35.21619445302929	38.0	34.0	40.0	26.0	41.0
39	35.17447952421519	38.0	34.0	40.0	26.0	41.0
40	35.00727470699465	38.0	34.0	40.0	25.0	41.0
41	34.9220954845714	38.0	34.0	40.0	24.0	41.0
42	34.86698890319598	38.0	34.0	40.0	24.0	41.0
43	34.820411800472755	37.0	34.0	40.0	24.0	41.0
44	34.702746873805594	37.0	34.0	40.0	24.0	41.0
45	34.59232961849534	37.0	33.0	40.0	24.0	41.0
46	34.527925428289564	37.0	33.0	40.0	24.0	41.0
47	34.38381222636357	37.0	33.0	40.0	23.0	41.0
48	34.338472401649916	37.0	33.0	40.0	23.0	41.0
49	34.22947584259746	36.0	33.0	40.0	24.0	41.0
50	34.13481718342726	36.0	33.0	40.0	23.0	41.0
51	33.676981380927366	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	7.0
12	5.0
13	8.0
14	27.0
15	72.0
16	193.0
17	434.0
18	962.0
19	1855.0
20	2979.0
21	4733.0
22	7173.0
23	10590.0
24	14862.0
25	21351.0
26	28303.0
27	34647.0
28	38288.0
29	43551.0
30	50662.0
31	61210.0
32	74670.0
33	95480.0
34	146205.0
35	191832.0
36	151740.0
37	189336.0
38	277670.0
39	437452.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.227327304562824	24.041997477734032	29.507596219039556	16.223078998663592
2	29.354977393435856	26.173094875474384	31.600896734996148	12.871030996093616
3	28.250386053003634	25.829212165612898	32.96836989417403	12.95203188720944
4	25.651201044953904	27.76321446311199	32.180678636916156	14.404905855017947
5	22.830747895858725	31.351320690903673	31.701087627803027	14.116843785434577
6	21.31532088386391	40.58425603491096	27.93804492050727	10.162378160717854
7	88.57124076083586	3.112385025649399	6.8107542465830395	1.5056199669317043
8	89.63681596523323	2.4851582300457116	6.436125125172352	1.4419006795487064
9	85.49045032360598	4.637460049544131	7.817752235873747	2.05433739097614
10	39.449872481908656	32.36992810110693	15.28685076699044	12.893348649993982
11	31.7815584083749	24.990736318244444	26.244500765744682	16.98320450763598
12	30.89023053977446	22.373315507482776	28.460044773136545	18.276409179606222
13	24.428236020057263	27.20659839196268	29.04830844375978	19.316857144220283
14	19.233788821983048	31.042425276943003	30.559070482967815	19.164715418106137
15	18.444136155712552	26.525777420365472	37.34771911986807	17.682367304053916
16	22.822372149264286	24.970698139683066	34.511945776052684	17.694983934999968
17	23.19175317628985	24.967305432201776	27.9048069894015	23.93613440210687
18	23.37103656225433	26.664295305500058	31.08668950736297	18.877978624882648
19	26.385033070946285	27.295444919128986	26.846388277347476	19.473133732577256
20	27.563733865422957	26.86997819655333	27.100417250009144	18.465870688014572
21	25.18571097630989	26.39738464662036	30.883710180083852	17.533194196985896
22	24.58355840943512	22.486335075453283	30.505423295919897	22.4246832191917
23	21.9282346947809	26.208294215592776	30.305624631904493	21.55784645772183
24	21.11112230233371	25.79523207974559	33.678028879362216	19.41561673855849
25	22.0820197635813	26.72398575274902	30.728970912304344	20.465023571365336
26	20.325445465142845	30.337007176106432	27.669278874723744	21.668268484026974
27	19.776250941608854	29.933327996887193	30.223351475483184	20.06706958602077
28	18.60359340733323	28.559546522236285	33.54067723742432	19.296182833006167
29	20.440691497397953	27.046929096124416	32.065856693096215	20.44652271338142
30	20.680195441155345	28.491109251012116	31.23300001749365	19.595695290338895
31	24.764697182303426	27.093525812937774	27.901520304029	20.2402567007298
32	25.89107606609206	26.890546485658657	27.634503623040512	19.58387382520877
33	24.450023563413676	27.12814203145782	27.872046157785284	20.54978824734322
34	21.53118189736106	27.800534245406194	29.465823508176157	21.20246034905659
35	21.596756571647884	27.04173401279369	29.81251580392059	21.548993611637833
36	25.397119061237838	26.870455296042884	28.162917813251596	19.56950782946768
37	20.999799088103842	29.64065396557144	28.977856752468593	20.381690193856127
38	22.429348191978473	29.530443983483877	26.68544671620373	21.354761108333918
39	21.716667576689765	28.308168102294374	28.4088891056452	21.566275215370663
40	22.838858587181186	26.89065250776744	28.714020734743816	21.556468170307554
41	20.1985370009208	25.895369961498073	29.999750848044343	23.906342189536783
42	21.9908407500216	26.789348382818268	28.087695127064848	23.132115740095283
43	21.080481912893294	27.009609313830214	29.123531129946528	22.786377643329963
44	21.73771296528465	27.62061472678898	28.692180180333004	21.949492127593366
45	20.8904372828866	30.228281503541933	27.17590499146787	21.705376222103595
46	22.135985016955587	28.30838014651195	28.258443733271697	21.29719110326076
47	21.94890900599502	27.785691150175545	28.55594177053741	21.709458073292023
48	21.39913136086268	27.426859322974618	29.6202447096293	21.553764606533402
49	21.77922062087607	26.465503851518157	29.793325802229536	21.961949725376233
50	21.179559573557874	28.329796612487602	28.986497554335006	21.50414625961952
51	20.74184729741693	29.41440278541284	28.27084832000017	21.57290159717006
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2758.0
1	3140.5
2	3523.0
3	14346.0
4	25169.0
5	16994.5
6	8820.0
7	9105.5
8	9391.0
9	10060.5
10	10730.0
11	10970.0
12	11210.0
13	10931.5
14	10653.0
15	9918.5
16	9184.0
17	8645.5
18	8107.0
19	7952.5
20	7798.0
21	8481.5
22	9165.0
23	10015.5
24	10866.0
25	11610.0
26	15533.0
27	18712.0
28	21796.0
29	24880.0
30	30760.0
31	36640.0
32	42593.0
33	48546.0
34	53409.5
35	58273.0
36	63748.5
37	69224.0
38	73285.5
39	77347.0
40	91094.0
41	104841.0
42	119031.5
43	133222.0
44	136946.0
45	140670.0
46	142440.5
47	144211.0
48	149686.0
49	155161.0
50	145921.5
51	136682.0
52	130435.0
53	124188.0
54	114734.0
55	105280.0
56	98865.0
57	92450.0
58	85490.0
59	78530.0
60	70174.5
61	61819.0
62	55448.5
63	49078.0
64	39901.5
65	30725.0
66	25099.0
67	19473.0
68	16577.5
69	13682.0
70	11189.5
71	8697.0
72	7714.5
73	6732.0
74	5171.5
75	2673.0
76	1735.0
77	1286.0
78	837.0
79	777.5
80	718.0
81	561.5
82	405.0
83	282.5
84	160.0
85	118.5
86	77.0
87	51.5
88	26.0
89	24.0
90	22.0
91	15.5
92	9.0
93	6.5
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1886399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.921315389464578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.02204469333296	16.339870212246453
2	6.595219756209135	2.627709056525397
3	2.1497241022926197	1.2847599552631466
4	1.078623658252572	0.8595040833035018
5	0.7082632154953514	0.7054767447319605
6	0.5009730381601343	0.5988025136883784
7	0.39429180006277403	0.5498367913171162
8	0.30738937920963333	0.48988806164854654
9	0.27613725184045246	0.4950915556224294
>10	3.1711765782203	15.473170938300434
>50	1.4960180803759373	21.83486098156806
>100	1.2950681200944372	37.01550079769279
>500	0.0034691707315163615	0.40955912519376503
>1k	0.0013342964351986007	0.43518315145848396
>5k	0.0	0.0
>10k+	2.6685928703972016E-4	0.8807860314395508
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16568	0.8782871492192267	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2110	0.11185332477381509	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.301105439517302E-5	0.0	0.0	0.03525235117279006	0.0
2	5.301105439517302E-5	0.0	0.0	0.1092557831084516	0.0
3	5.301105439517302E-5	0.0	0.0	0.15728379839047837	0.0
4	5.301105439517302E-5	0.0	0.0	0.26065535446106575	0.0
5	5.301105439517302E-5	0.0	0.0	0.4563721672880446	0.0
6	5.301105439517302E-5	0.0	0.0	0.7240779919836684	0.0
7	5.301105439517302E-5	0.0	0.0	0.8667837504154742	0.0
8	5.301105439517302E-5	0.0	0.0	1.1282342706924675	0.0
9	5.301105439517302E-5	0.0	0.0	1.2327720699597486	0.0
10	5.301105439517302E-5	0.0	0.0	1.411472334325877	0.0
11	5.301105439517302E-5	0.0	0.0	1.638147602919637	0.0
12	5.301105439517302E-5	0.0	0.0	1.8335463494202446	0.0
13	5.301105439517302E-5	0.0	0.0	1.9156074616239724	0.0
14	5.301105439517302E-5	0.0	0.0	1.9487393706209555	0.0
15	5.301105439517302E-5	0.0	0.0	1.9901410041035856	0.0
16	5.301105439517302E-5	0.0	0.0	2.0760719232781613	0.0
17	5.301105439517302E-5	0.0	0.0	2.180450689382257	0.0
18	5.301105439517302E-5	0.0	0.0	2.31165304901031	0.0
19	5.301105439517302E-5	0.0	0.0	2.3901624205695615	0.0
20	5.301105439517302E-5	0.0	0.0	2.480811323585307	0.0
21	5.301105439517302E-5	0.0	0.0	2.5946260573717437	0.0
22	5.301105439517302E-5	0.0	0.0	2.715120184011972	0.0
23	5.301105439517302E-5	0.0	0.0	2.8415515487444596	0.0
24	5.301105439517302E-5	0.0	0.0	2.9380316677436746	0.0
25	5.301105439517302E-5	0.0	0.0	3.024757752734178	0.0
26	5.301105439517302E-5	0.0	0.0	3.1040092790549614	0.0
27	5.301105439517302E-5	0.0	0.0	3.1946581820707074	0.0
28	5.301105439517302E-5	0.0	0.0	3.285943217739195	0.0
29	5.301105439517302E-5	0.0	0.0	3.3972664319690584	0.0
30	1.0602210879034604E-4	0.0	0.0	3.529847079011386	0.0
31	1.0602210879034604E-4	0.0	0.0	3.640110072153346	0.0
32	1.0602210879034604E-4	0.0	0.0	3.746609280433249	0.0
33	1.0602210879034604E-4	0.0	0.0	3.8577734614999266	0.0
34	1.0602210879034604E-4	0.0	0.0	3.9707930294704354	0.0
35	1.0602210879034604E-4	0.0	0.0	4.10867478195228	0.0
36	1.0602210879034604E-4	0.0	0.0	4.22498103529529	0.0
37	1.0602210879034604E-4	0.0	0.0	4.345210106663543	0.0
38	1.0602210879034604E-4	0.0	0.0	4.487173710333816	0.0
39	1.0602210879034604E-4	0.0	0.0	4.728533040995039	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCTTA	30	2.1662454E-6	45.000004	40
ACGATAA	25	3.8917606E-5	45.0	10
CCGTAGA	20	7.034439E-4	45.0	19
TCGAGTA	45	3.8562575E-10	45.0	27
CGCGTAT	20	7.034439E-4	45.0	26
CGACTAG	20	7.034439E-4	45.0	26
TAGTACG	55	1.8189894E-12	45.0	1
ACGCGTA	20	7.034439E-4	45.0	25
TATAGCG	130	0.0	44.999996	1
TACGCGG	165	0.0	43.636364	2
ATATGCG	100	0.0	42.75	1
CGTTTTT	7440	0.0	42.308468	1
CGGTCTA	410	0.0	41.707317	31
GGCGATA	560	0.0	41.383926	8
TAACGCC	240	0.0	41.250004	12
ATAACGC	240	0.0	41.250004	11
TCTCACG	445	0.0	40.955055	23
CGGGTTA	110	0.0	40.909092	6
CGAGTAC	50	1.0822987E-9	40.5	28
ATATCGG	50	1.0822987E-9	40.5	2
>>END_MODULE