FastQCFastQC Report
Thu 26 May 2016
SRR1547323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547323_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1150538
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70170.6098885912503541No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT11720.10186538819230656No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG351.211938E-745.01
GTATGCG551.8189894E-1245.01
ATAACGC750.045.011
CGTTTTT40450.042.886281
CACGACG1900.042.6315826
CGGTCTA1850.042.56756631
CGACGGT1950.041.5384628
TCACGAC2000.040.525
ACGGGCC950.040.2631575
CGGGATA2250.040.0000046
TAGGGCG4400.038.8636365
TACGGGA2850.038.684214
TAATGCG356.248296E-638.5714261
TGCGGGA6950.038.525184
ATAGGGC6550.038.4732864
GCGCGAC1950.038.0769239
GGGCGAT14900.037.9026877
ACGGTCT2200.037.84090830
TAGGGAC9400.037.819155
GTAGGGA14000.037.767864
Produced by FastQC (version 0.11.5)