##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547323_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1150538
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.296762905701506	31.0	31.0	33.0	30.0	34.0
2	31.724617526757047	31.0	31.0	34.0	30.0	34.0
3	31.860664315302927	31.0	31.0	34.0	30.0	34.0
4	35.63600159229856	37.0	35.0	37.0	33.0	37.0
5	35.51237508017988	37.0	35.0	37.0	33.0	37.0
6	35.549155264754404	37.0	35.0	37.0	33.0	37.0
7	35.91765852149168	37.0	35.0	37.0	35.0	37.0
8	35.89433551955694	37.0	35.0	37.0	35.0	37.0
9	37.631694042265444	39.0	37.0	39.0	35.0	39.0
10	36.92287434226423	39.0	37.0	39.0	32.0	39.0
11	36.696290778748725	39.0	35.0	39.0	32.0	39.0
12	36.37279776939136	38.0	35.0	39.0	32.0	39.0
13	36.22760047908022	39.0	35.0	39.0	32.0	39.0
14	37.256940666018856	39.0	36.0	41.0	32.0	41.0
15	37.382413271008865	39.0	36.0	41.0	32.0	41.0
16	37.43070111547815	39.0	36.0	41.0	32.0	41.0
17	37.34724450648305	39.0	35.0	41.0	32.0	41.0
18	37.244350034505594	39.0	36.0	41.0	32.0	41.0
19	37.217479996314765	39.0	35.0	41.0	32.0	41.0
20	37.08860376623806	39.0	35.0	41.0	31.0	41.0
21	36.98709125643829	39.0	35.0	41.0	31.0	41.0
22	36.89644236000897	39.0	35.0	41.0	31.0	41.0
23	36.81828935680525	39.0	35.0	41.0	31.0	41.0
24	36.681309091920475	38.0	35.0	40.0	31.0	41.0
25	36.58297596428801	38.0	35.0	40.0	31.0	41.0
26	36.48192497770608	38.0	35.0	40.0	30.0	41.0
27	36.41488155975726	38.0	35.0	40.0	30.0	41.0
28	36.48689569575277	38.0	35.0	40.0	30.0	41.0
29	36.50816574506883	38.0	35.0	40.0	30.0	41.0
30	36.46396468434767	38.0	35.0	40.0	30.0	41.0
31	36.37359044203668	38.0	35.0	40.0	30.0	41.0
32	36.2901016741733	38.0	35.0	40.0	30.0	41.0
33	36.13382434999974	38.0	35.0	40.0	30.0	41.0
34	35.93078629302118	38.0	35.0	40.0	29.0	41.0
35	35.7961631862659	38.0	35.0	40.0	28.0	41.0
36	35.66748164771611	38.0	34.0	40.0	27.0	41.0
37	35.59987675331019	38.0	34.0	40.0	27.0	41.0
38	35.49909955168799	38.0	34.0	40.0	27.0	41.0
39	35.45781451807763	38.0	34.0	40.0	27.0	41.0
40	35.30182401624283	38.0	34.0	40.0	26.0	41.0
41	35.205609028124236	38.0	34.0	40.0	26.0	41.0
42	35.13017214555278	38.0	34.0	40.0	26.0	41.0
43	35.09972899634779	38.0	34.0	40.0	26.0	41.0
44	34.987545826387304	38.0	34.0	40.0	25.0	41.0
45	34.870747424248485	38.0	34.0	40.0	25.0	41.0
46	34.79703321402683	37.0	34.0	40.0	24.0	41.0
47	34.654961418049645	37.0	33.0	40.0	24.0	41.0
48	34.59267838176575	37.0	33.0	40.0	24.0	41.0
49	34.47350804580118	37.0	33.0	40.0	24.0	41.0
50	34.392963118123866	37.0	33.0	40.0	24.0	41.0
51	33.949538389866305	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	4.0
13	9.0
14	24.0
15	38.0
16	96.0
17	249.0
18	453.0
19	945.0
20	1618.0
21	2636.0
22	3906.0
23	5736.0
24	8277.0
25	12077.0
26	16242.0
27	19881.0
28	22142.0
29	25151.0
30	30222.0
31	36332.0
32	44619.0
33	56909.0
34	84873.0
35	105602.0
36	94111.0
37	119964.0
38	179192.0
39	279160.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.40669843151639	23.253121583120244	30.440541729173653	14.899638256189712
2	31.02070509622455	25.460349853720608	30.28044271462568	13.238502335429164
3	28.19281066770502	25.940299233923607	32.577541984706286	13.289348113665087
4	25.02872569180679	28.475808708621532	32.3569495314366	14.138516068135083
5	22.70068437548347	32.72156156511128	30.63471176093271	13.943042298472541
6	20.377771094913857	41.081737413279704	27.957007938894673	10.583483552911769
7	89.46336409575346	3.0328420269473932	6.013099958454219	1.4906939188449229
8	90.70600014949528	2.4407711870446698	5.458402938451402	1.3948257250086482
9	86.64216218847183	4.390380847916366	6.762488505377484	2.2049684582343216
10	38.0932224750508	31.87039454585594	15.728554815225573	14.307828163867686
11	30.970815392451183	26.429548611171473	24.586150131503697	18.01348586487365
12	30.071583902487358	22.799942287868806	27.620817391515967	19.507656418127866
13	25.052366805789987	25.198385450980325	28.52500308551304	21.22424465771665
14	20.455560789821806	29.106644022187883	29.276912192383044	21.16088299560727
15	21.003043793425334	27.376409992542623	33.089302569754324	18.53124364427772
16	24.624306194145696	25.583248880089144	31.802860922455405	17.989584003309755
17	24.693665050611106	25.992361834202782	27.217440884177662	22.09653223100845
18	24.804830435848274	27.339210004363174	28.787662815135178	19.06829674465337
19	26.504991577853144	28.06826023999207	26.02313004872503	19.403618133429752
20	27.77552762272954	27.842539750968676	26.285702862486943	18.09622976381484
21	27.238213774773197	26.593732671150367	28.21766860373147	17.95038495034497
22	24.976228512226452	23.65649809045855	29.511845762591065	21.855427634723927
23	24.033539092146455	26.498646720056186	28.625999315103023	20.84181487269434
24	23.247993547366537	26.340720602014013	30.559181878390806	19.852103972228644
25	23.69830461923031	26.906108272825406	28.27181718465622	21.12376992328806
26	22.240551811413443	29.64665226181143	26.22903372161545	21.883762205159673
27	21.362440875486076	28.80313383825654	29.27856359372746	20.555861692529927
28	19.61343302003063	29.16896269397447	30.412815569759537	20.80478871623536
29	21.36218012790538	27.69495662029416	29.809184920445915	21.13367833135455
30	22.386483540743548	27.09428111022843	29.77389708119158	20.745338267836438
31	24.89409302430689	26.813369049957497	27.413870728302758	20.878667197432854
32	25.137283601236987	26.966514795686887	27.755189311435174	20.14101229164095
33	24.32488105564527	27.25342405031385	26.907064347287964	21.51463054675291
34	21.61362771155755	27.77526687514884	29.205815018712983	21.40529039458062
35	21.56504174568767	27.567798716774238	28.947674913822922	21.919484623715167
36	24.007551249936988	29.013817883459737	26.99267646961682	19.985954396986454
37	22.881990859928138	29.22780473135177	27.19988388041073	20.690320528309364
38	22.74996566823521	29.45526353757981	26.269449596623495	21.52532119756149
39	22.425421846127637	28.137706012317715	26.981203576066154	22.455668565488494
40	23.892213903408667	26.46561869316789	28.0194135265415	21.622753876881944
41	21.17044374023283	26.76756439161505	28.562203073692483	23.499788794459636
42	22.357540559286175	28.001335027613166	27.04821570430529	22.592908708795363
43	22.178059307906388	27.114445589802337	27.481838931004454	23.225656171286822
44	22.51303303324184	27.606302442857167	27.34555486216014	22.535109661740854
45	21.917050979628662	28.360645193813678	26.913061541643994	22.809242284913665
46	22.854699279815183	28.052093889988853	26.768433550217374	22.324773279978587
47	22.40369288106955	27.97282662545696	27.5187781716032	22.10470232187029
48	22.032996737178607	27.486793135037697	28.623218007575584	21.856992120208112
49	22.40021624666026	27.155209128251308	28.353518093274626	22.091056531813813
50	21.319852103972227	27.616123935063424	28.942720709789683	22.121303251174666
51	20.641908394159948	28.694662844686576	27.59934917403858	23.064079587114897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1105.0
1	1319.5
2	1534.0
3	6667.5
4	11801.0
5	8153.0
6	4505.0
7	4546.5
8	4588.0
9	4869.0
10	5150.0
11	5248.0
12	5346.0
13	5201.5
14	5057.0
15	4816.5
16	4576.0
17	4366.0
18	4156.0
19	4148.5
20	4141.0
21	4424.5
22	4708.0
23	5131.0
24	5554.0
25	6477.5
26	9331.5
27	11262.0
28	12863.0
29	14464.0
30	16484.5
31	18505.0
32	22945.0
33	27385.0
34	30073.5
35	32762.0
36	36105.0
37	39448.0
38	44671.5
39	49895.0
40	53580.5
41	57266.0
42	64278.0
43	71290.0
44	75720.0
45	80150.0
46	86126.5
47	92103.0
48	93634.0
49	95165.0
50	91378.5
51	87592.0
52	83603.0
53	79614.0
54	75831.0
55	72048.0
56	67713.0
57	63378.0
58	59692.0
59	56006.0
60	50644.0
61	45282.0
62	38713.0
63	32144.0
64	26134.5
65	20125.0
66	16295.5
67	12466.0
68	10894.5
69	9323.0
70	7340.5
71	5358.0
72	4427.5
73	3497.0
74	2827.5
75	1632.0
76	1106.0
77	842.5
78	579.0
79	463.5
80	348.0
81	213.5
82	79.0
83	53.0
84	27.0
85	40.5
86	54.0
87	36.5
88	19.0
89	14.5
90	10.0
91	7.0
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1150538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.834361383499783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.36178705793448	18.43868790012167
2	5.84892631831255	2.788108471522484
3	2.272118892749108	1.6246350838817887
4	1.377225647181732	1.3130117512621509
5	0.9858829303441716	1.1748945021823367
6	0.7831644333605366	1.1199734476451317
7	0.6621397132264297	1.1047174047983948
8	0.5764800858754924	1.099202775771798
9	0.49391478977573916	1.0594929232953263
>10	8.315796495827984	48.30775426535176
>50	1.2720198067997404	19.231668214927005
>100	0.04798001121870651	1.7265332321924804
>500	0.0018312981381185074	0.29645600447595954
>1k	3.662596276237015E-4	0.10231049388864093
>5k	3.662596276237015E-4	0.6125535286831001
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7017	0.6098885912503541	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	1172	0.10186538819230656	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.691586023234348E-5	0.0	0.0	0.02581401048900601	0.0
2	8.691586023234348E-5	0.0	0.0	0.07961492797282663	0.0
3	8.691586023234348E-5	0.0	0.0	0.12246444706737196	0.0
4	8.691586023234348E-5	0.0	0.0	0.20477376670740124	0.0
5	8.691586023234348E-5	0.0	0.0	0.3690447425465304	0.0
6	8.691586023234348E-5	0.0	0.0	0.6869829592764428	0.0
7	8.691586023234348E-5	0.0	0.0	0.8606408480206651	0.0
8	8.691586023234348E-5	0.0	0.0	1.1681491615226964	0.0
9	8.691586023234348E-5	0.0	0.0	1.3245107940806822	0.0
10	8.691586023234348E-5	0.0	0.0	1.5607481021921918	0.0
11	8.691586023234348E-5	0.0	0.0	1.7839480312688498	0.0
12	8.691586023234348E-5	0.0	0.0	1.9620386288849216	0.0
13	8.691586023234348E-5	0.0	0.0	2.0503451428809827	0.0
14	8.691586023234348E-5	0.0	0.0	2.102842322461318	0.0
15	8.691586023234348E-5	0.0	0.0	2.156556324084906	0.0
16	8.691586023234348E-5	0.0	0.0	2.23790956926238	0.0
17	8.691586023234348E-5	0.0	0.0	2.332647856915634	0.0
18	8.691586023234348E-5	0.0	0.0	2.454330061240915	0.0
19	8.691586023234348E-5	0.0	0.0	2.53907302496745	0.0
20	8.691586023234348E-5	0.0	0.0	2.627379538963511	0.0
21	8.691586023234348E-5	0.0	0.0	2.7415869793088103	0.0
22	8.691586023234348E-5	0.0	0.0	2.871091611055002	0.0
23	8.691586023234348E-5	0.0	0.0	3.0035513820490936	0.0
24	8.691586023234348E-5	0.0	0.0	3.1086326570699967	0.0
25	8.691586023234348E-5	0.0	0.0	3.203110197142554	0.0
26	8.691586023234348E-5	0.0	0.0	3.2958494200104647	0.0
27	8.691586023234348E-5	0.0	0.0	3.400148452289277	0.0
28	8.691586023234348E-5	0.0	0.0	3.5054904748908773	0.0
29	8.691586023234348E-5	0.0	0.0	3.627954921958249	0.0
30	8.691586023234348E-5	0.0	0.0	3.763630579780937	0.0
31	8.691586023234348E-5	0.0	0.0	3.8767950298034486	0.0
32	8.691586023234348E-5	0.0	0.0	4.007951062894055	0.0
33	8.691586023234348E-5	0.0	0.0	4.124418315605395	0.0
34	8.691586023234348E-5	0.0	0.0	4.250098649501363	0.0
35	8.691586023234348E-5	0.0	0.0	4.38012477640895	0.0
36	8.691586023234348E-5	0.0	0.0	4.498504178045401	0.0
37	8.691586023234348E-5	0.0	0.0	4.637134975115989	0.0
38	8.691586023234348E-5	0.0	0.0	4.8059255756872	0.0
39	8.691586023234348E-5	0.0	0.0	5.102047911498794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	35	1.211938E-7	45.0	1
GTATGCG	55	1.8189894E-12	45.0	1
ATAACGC	75	0.0	45.0	11
CGTTTTT	4045	0.0	42.88628	1
CACGACG	190	0.0	42.63158	26
CGGTCTA	185	0.0	42.567566	31
CGACGGT	195	0.0	41.53846	28
TCACGAC	200	0.0	40.5	25
ACGGGCC	95	0.0	40.263157	5
CGGGATA	225	0.0	40.000004	6
TAGGGCG	440	0.0	38.863636	5
TACGGGA	285	0.0	38.68421	4
TAATGCG	35	6.248296E-6	38.571426	1
TGCGGGA	695	0.0	38.52518	4
ATAGGGC	655	0.0	38.473286	4
GCGCGAC	195	0.0	38.076923	9
GGGCGAT	1490	0.0	37.902687	7
ACGGTCT	220	0.0	37.840908	30
TAGGGAC	940	0.0	37.81915	5
GTAGGGA	1400	0.0	37.76786	4
>>END_MODULE