FastQCFastQC Report
Thu 26 May 2016
SRR1547322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547322_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2339944
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172820.7385646835992656No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31790.13585795215611998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT253.8920487E-545.043
CGTAAGG3000.044.252
TAGTACG1250.043.21
CGTTTTT96050.042.352941
TCACGAC5050.042.32673325
ATTAGCG1650.042.2727281
CGACGGT5000.041.85000228
CACGACG5000.041.85000226
CGGTCTA5000.041.85000231
ACGGTCT5300.039.48113330
CATTACG403.4602635E-739.37500430
CGAATCG356.2510644E-638.57142626
CCGCGTA356.2510644E-638.57142642
GTATGCG2050.038.4146351
GGGCGAT28400.038.1073957
TACGGGA10050.038.05974
CTATGCG950.037.8947331
TGGGCGA8150.037.822096
ACGGGTC2500.037.8000035
CACGACC3400.037.7205927
Produced by FastQC (version 0.11.5)