FastQCFastQC Report
Thu 26 May 2016
SRR1547321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547321_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences825617
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67240.8144212146794458No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG15100.18289352084562213No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC10810.13093238147954803No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC9090.11009947711832485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG600.045.0000041
TATCACG253.8898484E-545.01
TCTAACG253.8898484E-545.01
TTCGCAT351.2113742E-745.025
CGAGGGT900.042.54
CGTAAGG1500.042.02
CGTTGAT700.041.78571325
ACGGGAC2050.041.7073175
TATAGCG603.6379788E-1241.2500041
CGTTTTT30300.040.9900971
TCGATAG556.002665E-1140.9090921
TAGACGG501.0804797E-940.52
CGTTAGG1500.040.52
TCATCGA900.040.016
AACACGT2050.039.51219641
CGACCAC4600.038.64130812
TACGGGA2100.038.571434
GGCGATT3850.038.571438
CTCACGA700.038.57142624
CGACGGT700.038.57142628
Produced by FastQC (version 0.11.5)