##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547321_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	825617
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34556701230716	31.0	31.0	33.0	30.0	34.0
2	31.752383974651686	31.0	31.0	34.0	30.0	34.0
3	31.831471493440663	31.0	31.0	34.0	30.0	34.0
4	35.61589090340921	37.0	35.0	37.0	33.0	37.0
5	35.52364231841156	37.0	35.0	37.0	33.0	37.0
6	35.55747156369115	37.0	35.0	37.0	33.0	37.0
7	35.88712683968475	37.0	35.0	37.0	35.0	37.0
8	35.86976164492737	37.0	35.0	37.0	35.0	37.0
9	37.6246455681024	39.0	37.0	39.0	35.0	39.0
10	36.99449502614409	39.0	37.0	39.0	33.0	39.0
11	36.70462696383432	39.0	35.0	39.0	32.0	39.0
12	36.149936350632316	38.0	35.0	39.0	32.0	39.0
13	35.87838186471451	38.0	35.0	39.0	31.0	39.0
14	36.949988917379365	39.0	35.0	41.0	31.0	41.0
15	37.138521856986955	39.0	35.0	41.0	32.0	41.0
16	37.27864372947747	39.0	35.0	41.0	32.0	41.0
17	37.17499639663428	39.0	35.0	41.0	32.0	41.0
18	37.0421575621626	39.0	35.0	41.0	32.0	41.0
19	36.976433382549054	38.0	35.0	41.0	32.0	41.0
20	36.82957715260223	38.0	35.0	41.0	31.0	41.0
21	36.71544069465624	38.0	35.0	40.0	31.0	41.0
22	36.59942443045625	38.0	35.0	40.0	31.0	41.0
23	36.51656639822097	38.0	35.0	40.0	31.0	41.0
24	36.372010266261476	38.0	35.0	40.0	31.0	41.0
25	36.286171433001016	38.0	35.0	40.0	30.0	41.0
26	36.045133518326296	38.0	35.0	40.0	30.0	41.0
27	36.024648232776215	38.0	35.0	40.0	30.0	41.0
28	36.107009666709864	38.0	35.0	40.0	30.0	41.0
29	36.12843727781768	38.0	35.0	40.0	30.0	41.0
30	36.11358172130661	38.0	35.0	40.0	30.0	41.0
31	36.01107050848032	38.0	35.0	40.0	30.0	41.0
32	35.81420925198972	38.0	35.0	40.0	29.0	41.0
33	35.64367012791646	38.0	34.0	40.0	29.0	41.0
34	35.45773766770791	38.0	34.0	40.0	27.0	41.0
35	35.3387587707133	38.0	34.0	40.0	27.0	41.0
36	35.104829479044156	38.0	34.0	40.0	26.0	41.0
37	35.04921773655339	38.0	34.0	40.0	26.0	41.0
38	34.96489292250523	37.0	34.0	40.0	26.0	41.0
39	34.83285591260839	37.0	34.0	40.0	25.0	41.0
40	34.67844533239989	37.0	34.0	40.0	24.0	41.0
41	34.648110443462286	37.0	34.0	40.0	24.0	41.0
42	34.56383044438281	37.0	33.0	40.0	24.0	41.0
43	34.4440400330904	37.0	33.0	40.0	24.0	41.0
44	34.35352227485626	36.0	33.0	40.0	23.0	41.0
45	34.199350304075615	36.0	33.0	40.0	23.0	41.0
46	34.11001711447317	36.0	33.0	40.0	23.0	41.0
47	34.06836220668906	36.0	33.0	40.0	23.0	41.0
48	33.97171569868353	35.0	33.0	40.0	23.0	41.0
49	33.85654365159632	36.0	33.0	40.0	23.0	41.0
50	33.709089081256806	35.0	33.0	40.0	23.0	41.0
51	33.23394140382284	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	9.0
14	10.0
15	32.0
16	64.0
17	195.0
18	440.0
19	886.0
20	1502.0
21	2351.0
22	3595.0
23	5182.0
24	7227.0
25	9763.0
26	12756.0
27	15210.0
28	16980.0
29	19221.0
30	22403.0
31	27454.0
32	34029.0
33	43780.0
34	69219.0
35	93656.0
36	69081.0
37	82683.0
38	115345.0
39	172484.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.66090693384463	23.92646953732784	28.412932388746842	14.99969114008069
2	31.69060230106696	26.169519280731862	28.704350806729995	13.43552761147118
3	28.81372355462642	26.369006452144273	31.35691246667644	13.460357526552869
4	24.823132275619326	28.81336019001547	31.98916688973216	14.374340644633044
5	23.77216069921041	31.934783319626415	29.690764603926517	14.602291377236662
6	22.30453103557703	41.79746783314782	26.083401867936345	9.814599263338811
7	88.61966262807088	2.9545176516471923	7.218237996552881	1.2075817237290414
8	88.86008887898383	3.309524876546874	6.3529457363402155	1.477440508129072
9	83.94097989745852	5.118717274474726	7.721497982720801	3.2188048453459657
10	42.66930065635761	32.53457717077047	13.659360211817345	11.136761961054582
11	37.0755447138322	23.508115748585602	23.782092665243084	15.63424687233911
12	33.11111568681362	21.78964338185866	27.650472313433465	17.448768617894252
13	23.805348000343983	29.831265586827794	28.804881682426597	17.558504730401626
14	20.380999906736417	31.60230470060573	29.370034773993268	18.646660618664587
15	18.39012520333278	24.45601289702126	38.71819499840725	18.435666901238708
16	21.5559999370168	22.812393640150336	36.93056223406253	18.70104418877034
17	22.48645558412678	22.050054686373947	28.92685107016934	26.53663865932993
18	23.07668083384911	23.87341830412891	31.835342537762667	21.21455832425931
19	26.91526458394146	25.092022087723485	27.36087071850507	20.63184260982998
20	28.619202366230347	25.33523413398707	26.552747823748785	19.492815676033803
21	25.863687399847628	26.269807913354498	28.974088469593045	18.89241621720483
22	25.271160840922608	23.75241788868204	28.018560664327406	22.957860606067946
23	23.061177277115174	27.143336438082066	27.773774038083033	22.021712246719726
24	21.770627300552192	25.496446899712577	33.170586361472694	19.562339438262537
25	21.188880558418735	26.726193864709664	31.41977454437106	20.665151032500543
26	20.627966720646498	29.91786748577125	28.030793939562777	21.42337185401948
27	21.319207332213363	29.00473221844996	28.479064747940026	21.196995701396652
28	19.11758115445782	27.71054859577746	33.549333407621205	19.62253684214351
29	20.509752100550255	25.485303718310064	32.6611491769186	21.343795004221086
30	21.196995701396652	28.1221195784486	30.471635152861438	20.20924956729331
31	26.092122618599177	26.1054459876674	26.675686183787402	21.126745209946016
32	26.8196996912612	26.053969334449267	27.625279033740824	19.501051940548706
33	25.33886778009658	27.11160259539229	26.563891005151298	20.985638619359825
34	21.318359481454475	27.485747023135428	28.90202115508765	22.293872340322448
35	21.78491964191629	26.76204583965689	29.4823144387773	21.97072007964952
36	27.02354723800503	26.59429251093425	26.59671494167392	19.785445309386798
37	22.45908211676843	29.426598531764732	27.8437822864597	20.27053706500714
38	22.49953671012104	29.273379787480152	26.145052730261124	22.08203077213769
39	22.563246638574544	28.473493157238767	27.001503118273966	21.961757085912716
40	24.377647262592703	26.066081488147653	26.681621139099608	22.87465011016004
41	20.676173092366074	25.318882726494245	28.66995229022658	25.3349918909131
42	22.689455280111723	26.962986469513105	26.369612059829194	23.97794619054598
43	22.24457587477002	26.576245401923654	27.204987300406845	23.97419142289948
44	21.47775542412523	28.155791365730114	28.357337603271247	22.009115606873404
45	20.725590679455486	29.948026748480228	26.429203856025254	22.897178716039036
46	22.72203697356038	29.14886684746075	26.730554240041084	21.398541938937786
47	21.892112202147	27.112571567688164	28.913164336490166	22.08215189367467
48	22.571482903089446	25.61962750282516	29.693550399277147	22.115339194808247
49	21.5874915366326	25.422199397541473	30.24525900023861	22.745050065587314
50	21.389942309811936	29.23147173568374	28.120181633856863	21.258404320647468
51	20.943488324489444	29.243705010919108	26.590779986361714	23.22202667822974
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	962.0
1	1246.0
2	1530.0
3	5997.5
4	10465.0
5	7112.5
6	3760.0
7	3992.5
8	4225.0
9	4654.0
10	5083.0
11	5316.5
12	5550.0
13	5355.0
14	5160.0
15	4803.5
16	4447.0
17	4226.5
18	4006.0
19	3998.0
20	3990.0
21	3961.5
22	3933.0
23	3936.5
24	3940.0
25	4716.0
26	6485.5
27	7479.0
28	8593.0
29	9707.0
30	11792.5
31	13878.0
32	15446.5
33	17015.0
34	19518.0
35	22021.0
36	23463.0
37	24905.0
38	28096.0
39	31287.0
40	37240.0
41	43193.0
42	50200.5
43	57208.0
44	62732.5
45	68257.0
46	64986.0
47	61715.0
48	60119.0
49	58523.0
50	56301.5
51	54080.0
52	51776.0
53	49472.0
54	46910.5
55	44349.0
56	42921.0
57	41493.0
58	37719.0
59	33945.0
60	33161.0
61	32377.0
62	29692.5
63	27008.0
64	23505.0
65	20002.0
66	17476.0
67	14950.0
68	12763.5
69	10577.0
70	8826.5
71	7076.0
72	5858.5
73	4641.0
74	4106.0
75	2863.5
76	2156.0
77	1611.0
78	1066.0
79	828.0
80	590.0
81	442.5
82	295.0
83	211.5
84	128.0
85	99.5
86	71.0
87	50.5
88	30.0
89	19.0
90	8.0
91	4.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	825617.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.74336259666894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94431198892877	18.50660061901026
2	5.19619781884453	2.467504178736919
3	2.0174828824833946	1.4370548283412823
4	1.2350788756760482	1.1729970232265046
5	0.878769306215022	1.0432469138143232
6	0.7002476675787201	0.997574056727595
7	0.59229079701753	0.984408260938008
8	0.5646553242385971	1.0725452884429343
9	0.4881428724641575	1.0431137897906415
>10	9.407105737372943	54.954787629582
>50	0.9336511288686891	13.466096086254211
>100	0.0389876295571367	1.4476202500526425
>500	0.001538985377255396	0.27186271755300784
>1k	0.0010259902515035975	0.31558974067197276
>5k	5.129951257517988E-4	0.8189986168577171
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6724	0.8144212146794458	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCG	1510	0.18289352084562213	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC	1081	0.13093238147954803	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGC	909	0.11009947711832485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029674776561044648	0.0
2	0.0	0.0	0.0	0.11724564780037233	0.0
3	0.0	0.0	0.0	0.1630295887802698	0.0
4	0.0	0.0	0.0	0.28184981656143226	0.0
5	0.0	0.0	0.0	0.5492861702217856	0.0
6	0.0	0.0	0.0	0.761369981480517	0.0
7	0.0	0.0	0.0	0.9096227427487564	0.0
8	1.211215369838557E-4	0.0	0.0	1.1603443243053377	0.0
9	1.211215369838557E-4	0.0	0.0	1.2560303385225837	0.0
10	1.211215369838557E-4	0.0	0.0	1.5950495205403958	0.0
11	1.211215369838557E-4	0.0	0.0	1.7975647303774025	0.0
12	1.211215369838557E-4	0.0	0.0	2.086924082231834	0.0
13	1.211215369838557E-4	0.0	0.0	2.1782497211176612	0.0
14	1.211215369838557E-4	0.0	0.0	2.221853474431849	0.0
15	1.211215369838557E-4	0.0	0.0	2.2776905029814065	0.0
16	1.211215369838557E-4	0.0	0.0	2.367078197275492	0.0
17	1.211215369838557E-4	0.0	0.0	2.459615051531158	0.0
18	1.211215369838557E-4	0.0	0.0	2.572742567074079	0.0
19	1.211215369838557E-4	0.0	0.0	2.6737579289186146	0.0
20	1.211215369838557E-4	0.0	0.0	2.755999452530653	0.0
21	1.211215369838557E-4	0.0	0.0	2.8551979913204306	0.0
22	1.211215369838557E-4	0.0	0.0	2.970990180676997	0.0
23	1.211215369838557E-4	0.0	0.0	3.087266856181498	0.0
24	1.211215369838557E-4	0.0	0.0	3.183195113472712	0.0
25	1.211215369838557E-4	0.0	0.0	3.266163366306653	0.0
26	1.211215369838557E-4	0.0	0.0	3.3497372268255137	0.0
27	1.211215369838557E-4	0.0	0.0	3.433311087344374	0.0
28	1.211215369838557E-4	0.0	0.0	3.513251301753719	0.0
29	1.211215369838557E-4	0.0	0.0	3.6072416144531907	0.0
30	1.211215369838557E-4	0.0	0.0	3.731512311398627	0.0
31	1.211215369838557E-4	0.0	0.0	3.842096274664887	0.0
32	1.211215369838557E-4	0.0	0.0	3.936934438123246	0.0
33	1.211215369838557E-4	0.0	0.0	4.030440264674783	0.0
34	1.211215369838557E-4	0.0	0.0	4.13436254340693	0.0
35	1.211215369838557E-4	0.0	0.0	4.2604500634071245	0.0
36	1.211215369838557E-4	0.0	0.0	4.363040005232451	0.0
37	1.211215369838557E-4	0.0	0.0	4.486341729882016	0.0
38	1.211215369838557E-4	0.0	0.0	4.598500273129066	0.0
39	1.211215369838557E-4	0.0	0.0	4.758986309632674	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	60	0.0	45.000004	1
TATCACG	25	3.8898484E-5	45.0	1
TCTAACG	25	3.8898484E-5	45.0	1
TTCGCAT	35	1.2113742E-7	45.0	25
CGAGGGT	90	0.0	42.5	4
CGTAAGG	150	0.0	42.0	2
CGTTGAT	70	0.0	41.785713	25
ACGGGAC	205	0.0	41.707317	5
TATAGCG	60	3.6379788E-12	41.250004	1
CGTTTTT	3030	0.0	40.990097	1
TCGATAG	55	6.002665E-11	40.909092	1
TAGACGG	50	1.0804797E-9	40.5	2
CGTTAGG	150	0.0	40.5	2
TCATCGA	90	0.0	40.0	16
AACACGT	205	0.0	39.512196	41
CGACCAC	460	0.0	38.641308	12
TACGGGA	210	0.0	38.57143	4
GGCGATT	385	0.0	38.57143	8
CTCACGA	70	0.0	38.571426	24
CGACGGT	70	0.0	38.571426	28
>>END_MODULE