FastQCFastQC Report
Thu 26 May 2016
SRR1547320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547320_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3150868
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233360.7406213145076214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAT207.035159E-445.00000435
CGACGTA253.8923587E-545.038
ACGTTAC253.8923587E-545.022
ATCGTCC650.041.53846410
CGTAAGG7250.040.965522
CGTTTTT123400.040.7151531
TAATGCG1400.040.178571
CGTTAGG4850.039.8969082
GGGCGAT55550.039.329437
GCGTAAG2750.039.2727241
GACCGAT18100.038.784539
CGGTCTA2450.038.5714331
TCGTAAG2050.038.4146351
TAAGGGA36400.037.9532974
ATAGGGC16900.037.943794
ATAGGGA35050.037.681884
TACGGGA10850.037.534564
AGGGCGA31300.037.523966
TAGGGAC31900.037.3824465
TAGGGTA16800.037.0982135
Produced by FastQC (version 0.11.5)