##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547320_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3150868
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.315432763289355	31.0	31.0	33.0	30.0	34.0
2	31.7210622596694	31.0	31.0	34.0	30.0	34.0
3	31.825007902584304	31.0	31.0	34.0	30.0	34.0
4	35.605063747513384	37.0	35.0	37.0	33.0	37.0
5	35.504481939579826	37.0	35.0	37.0	33.0	37.0
6	35.53530582683883	37.0	35.0	37.0	33.0	37.0
7	35.911720516378345	37.0	35.0	37.0	35.0	37.0
8	35.90956047666865	37.0	35.0	37.0	35.0	37.0
9	37.65125958942107	39.0	37.0	39.0	35.0	39.0
10	36.93607666204995	39.0	37.0	39.0	32.0	39.0
11	36.6756861918684	39.0	35.0	39.0	32.0	39.0
12	36.24784535562899	38.0	35.0	39.0	32.0	39.0
13	36.0382037584564	38.0	35.0	39.0	31.0	39.0
14	37.09996769144249	39.0	35.0	41.0	31.0	41.0
15	37.280891170306084	39.0	35.0	41.0	32.0	41.0
16	37.38889823375654	39.0	35.0	41.0	32.0	41.0
17	37.29048090875276	39.0	35.0	41.0	32.0	41.0
18	37.16550899625119	39.0	35.0	41.0	32.0	41.0
19	37.093105455385626	39.0	35.0	41.0	32.0	41.0
20	36.95049776759928	39.0	35.0	41.0	31.0	41.0
21	36.8150163700923	38.0	35.0	41.0	31.0	41.0
22	36.72547342510064	38.0	35.0	41.0	31.0	41.0
23	36.62545463662711	38.0	35.0	40.0	31.0	41.0
24	36.505189998438524	38.0	35.0	40.0	31.0	41.0
25	36.38515577294891	38.0	35.0	40.0	30.0	41.0
26	36.16438105309394	38.0	35.0	40.0	30.0	41.0
27	36.15887780763904	38.0	35.0	40.0	30.0	41.0
28	36.20938103405157	38.0	35.0	40.0	30.0	41.0
29	36.215817038352604	38.0	35.0	40.0	30.0	41.0
30	36.189244360601585	38.0	35.0	40.0	30.0	41.0
31	36.09408835914421	38.0	35.0	40.0	30.0	41.0
32	35.93648924677264	38.0	35.0	40.0	29.0	41.0
33	35.76928643154839	38.0	35.0	40.0	29.0	41.0
34	35.58075774675423	38.0	34.0	40.0	27.0	41.0
35	35.45779861295364	38.0	34.0	40.0	27.0	41.0
36	35.224009701453696	38.0	34.0	40.0	26.0	41.0
37	35.16841803591899	38.0	34.0	40.0	26.0	41.0
38	35.07419511068061	38.0	34.0	40.0	25.0	41.0
39	34.939409711863526	38.0	34.0	40.0	25.0	41.0
40	34.82178053793431	38.0	34.0	40.0	24.0	41.0
41	34.79219694382627	37.0	34.0	40.0	24.0	41.0
42	34.6999499820367	37.0	34.0	40.0	24.0	41.0
43	34.57893793075432	37.0	33.0	40.0	24.0	41.0
44	34.48546495759264	37.0	33.0	40.0	23.0	41.0
45	34.33401113597904	37.0	33.0	40.0	23.0	41.0
46	34.26372796321522	37.0	33.0	40.0	23.0	41.0
47	34.226550588599714	36.0	33.0	40.0	23.0	41.0
48	34.112335077191425	36.0	33.0	40.0	23.0	41.0
49	33.97959070326018	36.0	33.0	40.0	23.0	41.0
50	33.84796792502891	36.0	33.0	40.0	23.0	41.0
51	33.36592202529589	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	4.0
13	22.0
14	51.0
15	121.0
16	332.0
17	816.0
18	1811.0
19	3290.0
20	5577.0
21	8714.0
22	13089.0
23	19020.0
24	26666.0
25	37066.0
26	49244.0
27	59230.0
28	65663.0
29	74101.0
30	86267.0
31	103068.0
32	125941.0
33	159910.0
34	245780.0
35	326887.0
36	256604.0
37	320173.0
38	456160.0
39	705062.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.5981704089159	23.66827172702887	28.410964851590105	14.322593012465138
2	31.354376000517952	25.19051893002182	29.68778761915764	13.767317450302583
3	29.06662545051078	25.960021175117458	31.776323222680226	13.197030151691532
4	25.058491818762324	28.459427687862522	31.62668191749067	14.855398575884488
5	23.079545065042396	32.540207968090066	30.09684950305757	14.28339746380997
6	21.862610556837037	41.395323447380214	26.860534938309062	9.881531057473687
7	88.92343316190967	3.2397104543890762	6.569967386764536	1.266888996936717
8	89.61457604698134	2.409558255058606	6.545370989835182	1.4304947081248722
9	84.63083823251245	4.77804846156678	8.237856996865625	2.3532563090551557
10	40.32384092256483	30.610200109937956	15.845094113748972	13.220864853748237
11	33.777549551425196	24.765429716509864	24.76504886907354	16.6919718629914
12	31.132310207853838	22.782769700285762	27.541775790036272	18.54314430182413
13	23.608319993093964	27.77945632758973	29.650432833111385	18.961790846204917
14	20.07526180087519	30.586206721449454	30.251695723210236	19.08683575446512
15	19.144375454636627	25.189090752135602	37.5016027329612	18.164931060266568
16	22.54429573057329	23.251878529979674	35.872020027497186	18.33180571194985
17	22.71462974646986	23.649165880639874	29.15983786055144	24.476366512338824
18	24.06308356935295	24.102279118008116	31.945070374258776	19.889566938380156
19	26.760689435419067	25.304011466046816	27.868765051408058	20.066534047126062
20	27.35411321578689	25.60008861050352	27.89536089737812	19.150437276331473
21	25.296204093602142	26.60473241024378	29.34857950253708	18.750483993617
22	25.07927974132842	23.69965990323936	29.103726338266156	22.117334017166062
23	22.520175392939343	27.169751319318998	28.50750967669861	21.802563611043052
24	21.99352686307392	25.481645057806297	33.09792730130237	19.426900777817412
25	22.01206143830843	26.170026798964603	31.313466638399323	20.504445124327646
26	20.692742444304237	29.016544012633975	28.828913175670955	21.461800367390826
27	20.974633021757814	28.65616712601099	29.7779532497077	20.591246602523494
28	19.673721653842687	27.792659038715684	32.76973202304889	19.763887284392744
29	20.563635163389897	25.835293639720863	32.151807057610796	21.449264139278444
30	21.46700528235394	27.259186992282764	30.66926319985477	20.604544525508526
31	24.834394839771136	27.090058993267885	28.16170020451507	19.913845962445905
32	25.82472512336283	26.76024511341002	28.16782550078264	19.247204262444505
33	25.070012453711165	26.78944341686164	27.598331634330602	20.54221249509659
34	20.954257683914403	28.094734530294506	29.374032806198162	21.576974979592926
35	21.71274709064296	27.110846915833985	29.92235790264778	21.254048090875276
36	25.926855710870782	27.18073242039971	26.98034319431979	19.91206867440972
37	22.066459147130253	29.576834066041485	28.06604402342466	20.290662763403606
38	22.401033619942186	29.60644495421579	26.91252061336749	21.08000081247453
39	22.14088308364552	28.27896947761696	27.671898664114142	21.908248774623374
40	23.67519045545545	26.803693458437483	28.117363215469517	21.40375287063755
41	21.246177243857883	25.519348954002517	29.093411720199004	24.141062081940596
42	22.8977538887697	27.09209017959496	26.951779636595376	23.05837629503997
43	22.169668802374456	26.392378227205963	28.370182438616915	23.067770531802665
44	21.82963551630852	27.9245909381161	28.656706659879116	21.58906688569626
45	20.758565576215823	30.213737928723134	26.88449658951121	22.143199905549835
46	22.092420247373106	28.63807687278553	27.463892489307707	21.805610390533655
47	21.562471039726198	27.624737056582504	28.57412624076921	22.23866566292209
48	22.416267517395205	27.03813679278218	29.66668232372794	20.878913366094675
49	21.954172627986953	25.9737951573979	30.32570707500282	21.74632513961232
50	21.30406605417936	29.370763865703037	28.2135271931417	21.111642886975908
51	20.61574778759377	29.231437178580634	27.719504593654825	22.43331044017077
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3103.0
1	4511.5
2	5920.0
3	21541.5
4	37163.0
5	25664.0
6	14165.0
7	14920.0
8	15675.0
9	16778.5
10	17882.0
11	18689.5
12	19497.0
13	19006.5
14	18516.0
15	17758.5
16	17001.0
17	16566.0
18	16131.0
19	15907.5
20	15684.0
21	15784.0
22	15884.0
23	17290.5
24	18697.0
25	20793.0
26	27238.5
27	31588.0
28	36674.0
29	41760.0
30	48023.5
31	54287.0
32	63692.0
33	73097.0
34	81565.0
35	90033.0
36	98956.0
37	107879.0
38	119207.5
39	130536.0
40	154629.5
41	178723.0
42	197277.0
43	215831.0
44	232131.0
45	248431.0
46	246579.0
47	244727.0
48	234614.0
49	224501.0
50	214838.5
51	205176.0
52	196696.5
53	188217.0
54	181580.5
55	174944.0
56	164145.5
57	153347.0
58	142906.5
59	132466.0
60	120739.0
61	109012.0
62	98847.0
63	88682.0
64	78398.5
65	68115.0
66	58441.0
67	48767.0
68	42303.5
69	35840.0
70	30099.5
71	24359.0
72	20523.5
73	16688.0
74	13824.0
75	9153.0
76	7346.0
77	5757.0
78	4168.0
79	2958.5
80	1749.0
81	1312.0
82	875.0
83	545.0
84	215.0
85	187.5
86	160.0
87	129.5
88	99.0
89	71.5
90	44.0
91	30.0
92	16.0
93	15.5
94	15.0
95	11.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3150868.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.630627639030603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.24882087684074	15.323471555057463
2	6.68399807453919	2.4905415853347432
3	2.078834881942898	1.1618999582551883
4	1.0015525334168012	0.7463820924406472
5	0.6866421422921493	0.6396287037155651
6	0.4652270058534478	0.5200482668206018
7	0.33031649745229813	0.4307802566943797
8	0.2837234899913956	0.42287573555807345
9	0.2259570421250452	0.3788749362823622
>10	2.912114154185932	13.545207485322369
>50	1.6210783451230941	22.157200829431297
>100	1.456227433220042	40.617730110886505
>500	0.004302752356987183	0.5502707392226261
>1k	0.001032660565676924	0.2668144526001674
>5k	0.0	0.0
>10k+	1.721100942794873E-4	0.7482732923780202
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23336	0.7406213145076214	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02770665099267884	0.0
2	0.0	0.0	0.0	0.0834373258416411	0.0
3	0.0	0.0	0.0	0.119554357719841	0.0
4	0.0	0.0	0.0	0.21378236092403743	0.0
5	0.0	0.0	0.0	0.35475938693718684	0.0
6	0.0	0.0	0.0	0.6102445421388646	0.0
7	0.0	0.0	0.0	0.7261173746408927	0.0
8	0.0	0.0	0.0	0.9919488851960793	0.0
9	0.0	0.0	0.0	1.0889380323136355	0.0
10	0.0	0.0	0.0	1.2543527688243366	0.0
11	0.0	0.0	0.0	1.448045427482205	0.0
12	0.0	0.0	0.0	1.6067318592844892	0.0
13	0.0	0.0	0.0	1.6814096940906442	0.0
14	0.0	0.0	0.0	1.7189231665687041	0.0
15	0.0	0.0	0.0	1.7608163845645073	0.0
16	0.0	0.0	0.0	1.8453962527151249	0.0
17	0.0	0.0	0.0	1.9429884082735298	0.0
18	0.0	0.0	0.0	2.06387573202051	0.0
19	0.0	0.0	0.0	2.1358241601996655	0.0
20	0.0	0.0	0.0	2.2136757236418663	0.0
21	0.0	0.0	0.0	2.3210747006856525	0.0
22	0.0	0.0	0.0	2.439930838105563	0.0
23	0.0	0.0	0.0	2.5656739666656936	0.0
24	0.0	0.0	0.0	2.665678155987493	0.0
25	0.0	0.0	0.0	2.7549551425194583	0.0
26	0.0	0.0	0.0	2.840233230970006	0.0
27	0.0	0.0	0.0	2.9288437344884013	0.0
28	0.0	0.0	0.0	3.021072288651889	0.0
29	3.173728636045686E-5	0.0	0.0	3.1225998677189906	0.0
30	3.173728636045686E-5	0.0	0.0	3.255452148423863	0.0
31	3.173728636045686E-5	0.0	0.0	3.3683734132943686	0.0
32	3.173728636045686E-5	0.0	0.0	3.4831354407737805	0.0
33	6.347457272091373E-5	0.0	0.0	3.5999921291529824	0.0
34	6.347457272091373E-5	0.0	0.0	3.720244707172754	0.0
35	6.347457272091373E-5	0.0	0.0	3.858460589272543	0.0
36	6.347457272091373E-5	0.0	0.0	3.9844893534099177	0.0
37	6.347457272091373E-5	0.0	0.0	4.111724134428989	0.0
38	6.347457272091373E-5	0.0	0.0	4.250352601251465	0.0
39	6.347457272091373E-5	0.0	0.0	4.456264115158109	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGTAT	20	7.035159E-4	45.000004	35
CGACGTA	25	3.8923587E-5	45.0	38
ACGTTAC	25	3.8923587E-5	45.0	22
ATCGTCC	65	0.0	41.538464	10
CGTAAGG	725	0.0	40.96552	2
CGTTTTT	12340	0.0	40.715153	1
TAATGCG	140	0.0	40.17857	1
CGTTAGG	485	0.0	39.896908	2
GGGCGAT	5555	0.0	39.32943	7
GCGTAAG	275	0.0	39.272724	1
GACCGAT	1810	0.0	38.78453	9
CGGTCTA	245	0.0	38.57143	31
TCGTAAG	205	0.0	38.414635	1
TAAGGGA	3640	0.0	37.953297	4
ATAGGGC	1690	0.0	37.94379	4
ATAGGGA	3505	0.0	37.68188	4
TACGGGA	1085	0.0	37.53456	4
AGGGCGA	3130	0.0	37.52396	6
TAGGGAC	3190	0.0	37.382446	5
TAGGGTA	1680	0.0	37.098213	5
>>END_MODULE