FastQCFastQC Report
Thu 26 May 2016
SRR1547319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547319_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1730795
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451612.6092633731897767No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAC351.212411E-745.00000415
TATATCG207.034278E-445.01
TGTTACG600.044.9999961
CGTACAC302.1661417E-644.99999623
CGTTTTT156750.043.4928251
TAATGCG750.042.0000041
ATACCGG603.6379788E-1241.2499962
CTAACGG501.0822987E-940.52
GCTTACG501.0822987E-940.51
TAGGGTA8350.040.14975
CGACGGT2050.039.51219628
TACGGGA4050.039.4444474
TTAACGC403.4595723E-739.37531
CGTAATA403.4595723E-739.37525
ACGGGAT4800.039.3749965
TCTCACG1950.039.2307723
CGACAAT2300.039.13043220
GTATGCG750.039.0000041
GTTTTTT179450.038.743382
GGGCGAT31000.038.6129047
Produced by FastQC (version 0.11.5)