##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547319_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1730795
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238059966662718	31.0	31.0	33.0	30.0	34.0
2	31.621216261891213	31.0	31.0	34.0	30.0	34.0
3	31.598607576287197	31.0	31.0	34.0	30.0	34.0
4	35.44661037269001	37.0	35.0	37.0	33.0	37.0
5	35.36914077057075	37.0	35.0	37.0	33.0	37.0
6	35.42206616034828	37.0	35.0	37.0	33.0	37.0
7	35.87127129440517	37.0	35.0	37.0	35.0	37.0
8	35.881134391999055	37.0	35.0	37.0	35.0	37.0
9	37.61282300907964	39.0	37.0	39.0	35.0	39.0
10	36.89478881092215	39.0	37.0	39.0	32.0	39.0
11	36.58754618542346	39.0	35.0	39.0	32.0	39.0
12	36.164044268674225	38.0	35.0	39.0	32.0	39.0
13	35.93011592938505	38.0	35.0	39.0	31.0	39.0
14	37.018868785731414	39.0	35.0	41.0	31.0	41.0
15	37.216751839472614	39.0	35.0	41.0	32.0	41.0
16	37.334976123688826	39.0	35.0	41.0	32.0	41.0
17	37.24651561854523	39.0	35.0	41.0	32.0	41.0
18	37.050683645376836	39.0	35.0	40.0	32.0	41.0
19	36.89118757565165	38.0	35.0	40.0	31.0	41.0
20	36.67005624582923	38.0	35.0	40.0	31.0	41.0
21	36.52404935304297	38.0	35.0	40.0	31.0	41.0
22	36.417902755670085	38.0	35.0	40.0	31.0	41.0
23	36.34590982756479	38.0	35.0	40.0	31.0	41.0
24	36.17977692332136	38.0	35.0	40.0	30.0	41.0
25	36.01604869438611	37.0	35.0	40.0	30.0	41.0
26	35.77979541193498	37.0	34.0	40.0	30.0	41.0
27	35.708738469893895	37.0	34.0	40.0	30.0	41.0
28	35.65288494593525	37.0	34.0	40.0	30.0	41.0
29	35.60246765214829	37.0	34.0	40.0	29.0	41.0
30	35.51596058458685	37.0	34.0	40.0	29.0	41.0
31	35.35511253499114	37.0	34.0	40.0	28.0	41.0
32	35.08986737308578	37.0	34.0	40.0	27.0	41.0
33	34.76860691185264	37.0	33.0	40.0	25.0	41.0
34	34.445427101418716	36.0	33.0	40.0	23.0	41.0
35	34.1749565950907	36.0	33.0	40.0	23.0	41.0
36	33.88892214271476	36.0	33.0	40.0	21.0	41.0
37	33.80676394373684	36.0	33.0	40.0	21.0	41.0
38	33.7348403479326	36.0	33.0	40.0	21.0	41.0
39	33.613286379958346	36.0	33.0	40.0	20.0	41.0
40	33.48927111529673	35.0	33.0	40.0	20.0	41.0
41	33.45534624262261	35.0	33.0	40.0	20.0	41.0
42	33.36880277560312	35.0	33.0	40.0	19.0	41.0
43	33.240009937629814	35.0	33.0	40.0	18.0	41.0
44	33.136394547014525	35.0	32.0	40.0	18.0	41.0
45	32.98866070216288	35.0	32.0	40.0	18.0	41.0
46	32.89195658642416	35.0	32.0	39.0	18.0	41.0
47	32.83916639463368	35.0	32.0	39.0	18.0	41.0
48	32.69239915761254	35.0	31.0	39.0	18.0	41.0
49	32.5495954171349	35.0	31.0	39.0	18.0	41.0
50	32.40227063285947	35.0	31.0	39.0	18.0	41.0
51	31.992482067489217	35.0	31.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	12.0
14	33.0
15	81.0
16	236.0
17	598.0
18	1230.0
19	2419.0
20	4201.0
21	6559.0
22	9803.0
23	14279.0
24	20510.0
25	29655.0
26	38922.0
27	43851.0
28	44989.0
29	47548.0
30	52993.0
31	62137.0
32	74081.0
33	93211.0
34	140889.0
35	190315.0
36	167951.0
37	170090.0
38	213803.0
39	300297.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.18295349824792	22.484176346707727	27.008686759552692	19.324183395491666
2	35.99975733694632	23.911612871541692	27.5157947648335	12.572835026678492
3	26.58165756198741	24.702174434291756	35.911763091527305	12.80440491219353
4	23.54062728399377	27.384930046597084	35.43718349082358	13.637259178585564
5	21.116885593036727	31.187517874733867	34.39309681389188	13.302499718337527
6	20.655421352615416	39.47215008132101	30.47628401977126	9.39614454629231
7	83.59360871738131	3.1800993185212576	11.788282263352968	1.4380097007444554
8	83.84459164719104	2.659875952958034	11.578667606504526	1.9168647933464102
9	79.40189334958791	4.878451809717499	13.260264791613102	2.4593900490814913
10	40.273227043064026	28.10459933152106	19.440892768929885	12.181280856485026
11	32.14840579040268	24.887580562689397	27.63394856121031	15.330065085697614
12	30.144008966977605	22.604179004445935	29.95762063098172	17.294191397594748
13	21.31061159756066	29.715188684968467	31.735185276130334	17.23901444134054
14	17.746006892786262	32.32930531923191	31.873676547482514	18.05101124049931
15	16.863637808059302	27.211772624718698	38.99993933423658	16.92465023298542
16	19.15241261963433	25.59875664073446	38.04737129469405	17.201459444937154
17	19.43107069294746	26.4288376150844	30.4220892711153	23.718002420852848
18	20.575978090992866	27.668614711736517	33.40239600877054	18.353011188500084
19	23.364754346990836	30.061850190230498	28.417056901597242	18.156338561181425
20	25.196340410042783	28.698834928457735	28.491878009816297	17.61294665168319
21	21.799924312238016	30.329703979962964	30.54474966706051	17.325622040738505
22	21.70066356789799	28.05069346745282	29.964438307251868	20.28420465739732
23	20.353479181532187	30.41573381018549	28.756496292166318	20.474290716116002
24	20.14190010948726	28.431616684818252	34.06954607564732	17.356937130047175
25	19.208225121981517	29.686415780031723	32.207627130884944	18.897731967101823
26	18.58538995086073	32.869808382852966	28.820166455299447	19.72463521098686
27	18.550723800334527	32.58398597176442	29.677460357812453	19.187829870088603
28	17.88773367152089	31.603685011800938	32.647656134897545	17.860925181780626
29	18.53922619374334	28.795957926848644	32.84276878544253	19.822047093965487
30	19.3174812730566	31.17440251445145	30.916428577618955	18.59168763487299
31	23.45627298438001	30.399729604025893	27.667112511880376	18.476884899713717
32	24.72632518582501	30.193177123807267	27.089054451855937	17.99144323851178
33	23.467192821795766	31.489344491982006	26.75695272981491	18.286509956407315
34	20.628901747462873	31.061275309900942	28.363093260611453	19.94672968202473
35	20.074301115961163	31.076123977709663	29.350269673762636	19.499305232566538
36	24.4136942849962	30.97801877172051	26.824262838753288	17.78402410453
37	20.180552867323975	33.60906404282425	28.106390415964917	18.103992673886857
38	19.740177201806105	33.84063392833929	27.47280873818101	18.946380131673596
39	19.64680970305553	33.961387686005565	27.16370222932236	19.22810038161654
40	22.285597081110126	31.27204550510026	27.191608480495955	19.25074893329366
41	18.40882369084727	30.32398406512614	28.77637155180134	22.49082069222525
42	20.33608832935154	31.38806155552795	26.192472245413235	22.083377869707274
43	20.314768646777924	31.28868525735283	27.62984640006471	20.76669969580453
44	19.805118457125193	32.125121692632575	27.4080985905321	20.661661259710133
45	18.43245445012263	34.402745559121676	25.851010662730133	21.31378932802556
46	20.520974465491292	33.54435389517534	26.180396869646604	19.754274769686763
47	19.694706767699234	32.12650833865362	27.364823679291888	20.813961214355253
48	21.162702688648857	30.40781837248201	28.42399013170248	20.00548880716665
49	19.922347822821305	30.172030771986286	28.8735523271098	21.032069078082614
50	19.17084345633076	32.7612455547884	28.54041062055298	19.52750036832785
51	18.427485635213873	33.250789377135945	26.885333040596954	21.43639194705323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2006.0
1	2438.0
2	2870.0
3	29287.5
4	55705.0
5	34867.0
6	14029.0
7	15272.5
8	16516.0
9	20226.0
10	23936.0
11	27472.5
12	31009.0
13	31741.5
14	32474.0
15	30055.5
16	27637.0
17	24926.5
18	22216.0
19	20042.0
20	17868.0
21	16303.0
22	14738.0
23	14887.0
24	15036.0
25	15237.5
26	16398.5
27	17358.0
28	19337.0
29	21316.0
30	24123.5
31	26931.0
32	31640.5
33	36350.0
34	39077.5
35	41805.0
36	46486.5
37	51168.0
38	57368.5
39	63569.0
40	73971.0
41	84373.0
42	94106.5
43	103840.0
44	108996.0
45	114152.0
46	119134.0
47	124116.0
48	124860.0
49	125604.0
50	118131.5
51	110659.0
52	104178.5
53	97698.0
54	88803.0
55	79908.0
56	73420.5
57	66933.0
58	65659.5
59	64386.0
60	59739.0
61	55092.0
62	49373.5
63	43655.0
64	39148.5
65	34642.0
66	29838.5
67	25035.0
68	21944.5
69	18854.0
70	15636.0
71	12418.0
72	9831.0
73	7244.0
74	6113.5
75	4213.5
76	3444.0
77	2658.5
78	1873.0
79	1395.0
80	917.0
81	756.5
82	596.0
83	411.0
84	226.0
85	140.0
86	54.0
87	43.0
88	32.0
89	29.5
90	27.0
91	32.0
92	37.0
93	27.0
94	17.0
95	10.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1730795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.926061844116656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.59914082299908	21.16365326694507
2	7.447110934714399	4.010427391762346
3	3.1593782837612827	2.5520884517254627
4	1.8632428640065783	2.006791703474007
5	1.1846010439793977	1.594832048539721
6	0.8064879120879366	1.3029326038447377
7	0.6065913628312625	1.1433181584791101
8	0.4668434963646629	1.0056205483710847
9	0.3693124561528977	0.8949717030757989
>10	3.831705067645591	24.23684311068935
>50	1.3462141667145242	25.653707138908306
>100	0.31568319439836945	10.939021809802512
>500	0.0021696437318144976	0.35221394669299677
>1k	0.0013017862390886984	0.5052742453056442
>5k	0.0	0.0
>10k+	2.1696437318144974E-4	2.6383038723838825
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45161	2.6092633731897767	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1555383508734426E-4	0.0	0.0	0.057372479120866425	0.0
2	1.1555383508734426E-4	0.0	0.0	0.15305105457318746	0.0
3	1.1555383508734426E-4	0.0	0.0	0.23468983906239618	0.0
4	1.1555383508734426E-4	0.0	0.0	0.3602390808847957	0.0
5	1.1555383508734426E-4	0.0	0.0	0.6658789746908212	0.0
6	1.1555383508734426E-4	0.0	0.0	1.357584231523664	0.0
7	1.1555383508734426E-4	0.0	0.0	1.827021686566	0.0
8	1.1555383508734426E-4	0.0	0.0	2.5465754176548927	0.0
9	1.1555383508734426E-4	0.0	0.0	2.946507240892191	0.0
10	1.1555383508734426E-4	0.0	0.0	3.378794137953946	0.0
11	1.1555383508734426E-4	0.0	0.0	3.749433063996603	0.0
12	1.1555383508734426E-4	0.0	0.0	4.028322244979908	0.0
13	1.1555383508734426E-4	0.0	0.0	4.211128412088087	0.0
14	1.1555383508734426E-4	0.0	0.0	4.316051294347395	0.0
15	1.1555383508734426E-4	0.0	0.0	4.3972856404137985	0.0
16	1.1555383508734426E-4	0.0	0.0	4.5166527520590245	0.0
17	1.1555383508734426E-4	0.0	0.0	4.652139623698936	0.0
18	1.1555383508734426E-4	0.0	0.0	4.820443784503653	0.0
19	1.1555383508734426E-4	0.0	0.0	4.930162150919086	0.0
20	1.1555383508734426E-4	0.0	0.0	5.048893716471333	0.0
21	1.1555383508734426E-4	0.0	0.0	5.193336010330513	0.0
22	1.1555383508734426E-4	0.0	0.0	5.3441915420370405	0.0
23	1.1555383508734426E-4	0.0	0.0	5.511051279903166	0.0
24	1.1555383508734426E-4	0.0	0.0	5.652835835555337	0.0
25	1.1555383508734426E-4	0.0	0.0	5.793464852856635	0.0
26	1.1555383508734426E-4	0.0	0.0	5.913005295254493	0.0
27	1.1555383508734426E-4	0.0	0.0	6.028559130341837	0.0
28	1.1555383508734426E-4	0.0	0.0	6.149832880266005	0.0
29	1.1555383508734426E-4	0.0	0.0	6.288670813123449	0.0
30	1.1555383508734426E-4	0.0	0.0	6.479161310264936	0.0
31	1.1555383508734426E-4	0.0	0.0	6.629496849713571	0.0
32	1.7333075263101637E-4	0.0	0.0	6.772321389881529	0.0
33	1.7333075263101637E-4	0.0	0.0	6.914799268544224	0.0
34	1.7333075263101637E-4	0.0	0.0	7.059819331578841	0.0
35	1.7333075263101637E-4	0.0	0.0	7.235172276323886	0.0
36	1.7333075263101637E-4	0.0	0.0	7.382618969895337	0.0
37	2.311076701746885E-4	0.0	0.0	7.540696616294824	0.0
38	2.311076701746885E-4	0.0	0.0	7.71165851530655	0.0
39	2.311076701746885E-4	0.0	0.0	7.928726394518126	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGAC	35	1.212411E-7	45.000004	15
TATATCG	20	7.034278E-4	45.0	1
TGTTACG	60	0.0	44.999996	1
CGTACAC	30	2.1661417E-6	44.999996	23
CGTTTTT	15675	0.0	43.492825	1
TAATGCG	75	0.0	42.000004	1
ATACCGG	60	3.6379788E-12	41.249996	2
CTAACGG	50	1.0822987E-9	40.5	2
GCTTACG	50	1.0822987E-9	40.5	1
TAGGGTA	835	0.0	40.1497	5
CGACGGT	205	0.0	39.512196	28
TACGGGA	405	0.0	39.444447	4
TTAACGC	40	3.4595723E-7	39.375	31
CGTAATA	40	3.4595723E-7	39.375	25
ACGGGAT	480	0.0	39.374996	5
TCTCACG	195	0.0	39.23077	23
CGACAAT	230	0.0	39.130432	20
GTATGCG	75	0.0	39.000004	1
GTTTTTT	17945	0.0	38.74338	2
GGGCGAT	3100	0.0	38.612904	7
>>END_MODULE