FastQCFastQC Report
Thu 26 May 2016
SRR1547318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547318_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences738982
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159582.1594571992281275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA253.8894486E-545.09
CGTTTTT78650.043.2549251
CGGGTAT700.041.7857176
CGTAAGG650.041.5384642
TACGCGG603.6379788E-1241.2500042
TACGGGA2900.039.5689664
ACGTTAG403.455989E-739.3751
AATACGG403.455989E-739.3752
TATCCGG403.455989E-739.3752
GGGCGAT13750.039.109097
TTGGGCG1900.039.0789455
TTAGGGA11600.038.987074
ACGGGAT4050.038.888895
TAAGGGA9700.038.7371144
ACACGAC1000.038.2526
CGTTAGG1000.038.252
GTTTTTT92200.038.1426242
AGGGATC8200.038.1402446
TGCGATG301.1395272E-437.5000041
GCGATAA301.1395272E-437.5000049
Produced by FastQC (version 0.11.5)