##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547318_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	738982
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.401802750269965	31.0	31.0	33.0	30.0	34.0
2	31.806612339678097	31.0	31.0	34.0	30.0	34.0
3	31.76678457662027	31.0	31.0	34.0	30.0	34.0
4	35.61295674319537	37.0	35.0	37.0	33.0	37.0
5	35.565868992749486	37.0	35.0	37.0	33.0	37.0
6	35.614604956548334	37.0	35.0	37.0	33.0	37.0
7	35.97461778500694	37.0	35.0	37.0	35.0	37.0
8	35.97065693075068	37.0	35.0	37.0	35.0	37.0
9	37.74055930996966	39.0	38.0	39.0	35.0	39.0
10	37.105324622250606	39.0	37.0	39.0	33.0	39.0
11	36.82888487135005	39.0	37.0	39.0	32.0	39.0
12	36.59263013172175	39.0	35.0	39.0	32.0	39.0
13	36.509717422075234	39.0	35.0	39.0	32.0	39.0
14	37.6387990505858	40.0	36.0	41.0	32.0	41.0
15	37.7291990873932	40.0	36.0	41.0	32.0	41.0
16	37.781303198183444	40.0	36.0	41.0	33.0	41.0
17	37.673832380220354	40.0	36.0	41.0	32.0	41.0
18	37.44120560446669	39.0	36.0	41.0	32.0	41.0
19	37.26459237166805	39.0	36.0	41.0	32.0	41.0
20	37.03126057197604	39.0	35.0	41.0	32.0	41.0
21	36.9151589619233	38.0	35.0	41.0	31.0	41.0
22	36.833714488309596	38.0	35.0	40.0	31.0	41.0
23	36.707651877853586	38.0	35.0	40.0	31.0	41.0
24	36.588602970031744	38.0	35.0	40.0	31.0	41.0
25	36.47614150277003	38.0	35.0	40.0	31.0	41.0
26	36.28934940228585	38.0	35.0	40.0	30.0	41.0
27	36.26776565599703	38.0	35.0	40.0	30.0	41.0
28	36.250788246533745	38.0	35.0	40.0	30.0	41.0
29	36.20200627349516	38.0	35.0	40.0	30.0	41.0
30	36.1303401165387	38.0	35.0	40.0	30.0	41.0
31	35.99679288534768	38.0	35.0	40.0	30.0	41.0
32	35.72016774427523	38.0	35.0	40.0	29.0	41.0
33	35.354635160261005	38.0	34.0	40.0	26.0	41.0
34	34.94682549777938	38.0	34.0	40.0	24.0	41.0
35	34.670487237848825	38.0	34.0	40.0	23.0	41.0
36	34.42138509463018	38.0	33.0	40.0	21.0	41.0
37	34.370788463047816	38.0	33.0	40.0	21.0	41.0
38	34.25810506886501	38.0	33.0	40.0	21.0	41.0
39	34.09991447694261	38.0	33.0	40.0	20.0	41.0
40	33.99343962369855	37.0	33.0	40.0	19.0	41.0
41	33.925508334438454	37.0	33.0	40.0	18.0	41.0
42	33.830612924266084	37.0	33.0	40.0	18.0	41.0
43	33.698431084924934	37.0	33.0	40.0	18.0	41.0
44	33.598241905756836	37.0	33.0	40.0	18.0	41.0
45	33.44071168174597	37.0	33.0	40.0	18.0	41.0
46	33.366192951925754	36.0	33.0	40.0	18.0	41.0
47	33.31975068404914	36.0	32.0	40.0	18.0	41.0
48	33.18516283211228	36.0	32.0	40.0	18.0	41.0
49	33.05255608391003	36.0	32.0	40.0	18.0	41.0
50	32.94849671575221	35.0	32.0	40.0	18.0	41.0
51	32.48739617473768	35.0	31.0	39.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	4.0
14	13.0
15	17.0
16	65.0
17	165.0
18	449.0
19	804.0
20	1435.0
21	2226.0
22	3278.0
23	4951.0
24	7624.0
25	11589.0
26	16531.0
27	19265.0
28	19107.0
29	19094.0
30	20231.0
31	23217.0
32	27588.0
33	35027.0
34	51442.0
35	64492.0
36	64325.0
37	77644.0
38	107757.0
39	160586.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.265394826937595	22.501089336411443	26.63529016944932	21.598225667201636
2	37.7158036325648	23.637246915351117	26.369383828022876	12.277565624061209
3	26.891724020341496	24.131034314773565	36.44297154734486	12.534270117540075
4	23.82453158534308	26.554909321201333	36.439453193717846	13.181105899737746
5	21.744237342722826	30.656633043835978	34.36200069825787	13.23712891518332
6	20.87642188848984	38.826656129648626	31.103734597053784	9.19318738480775
7	83.89595416397152	2.408719021572921	12.661066169406022	1.034260645049541
8	84.23087436500482	2.1127713530234837	12.529669193566285	1.126685088405401
9	79.6089755907451	4.459107258363533	13.681524042534187	2.2503931083571724
10	35.83632618927119	34.28324370553004	19.485454314178153	10.39497579102062
11	26.72351965271143	24.90696661082408	32.28657802219811	16.082935714266384
12	25.47964632426771	21.821505801223847	34.83941422118536	17.859433653323084
13	22.686479508296546	23.0218056732099	36.37436365161803	17.917351166875513
14	20.122140999374814	25.301563502223328	35.65743143946673	18.91886405893513
15	19.01548346238474	24.8364642169904	38.410949116487274	17.737103204137583
16	23.181078835479077	24.07690579743485	36.007236982768184	16.734778384317885
17	23.209360985788557	23.844153172878364	33.676868990043054	19.269616851290017
18	22.842234316938708	24.12237375199937	35.05863471640717	17.976757214654754
19	23.853355020825948	25.508199117163883	32.58103715652073	18.057408705489443
20	25.467061443986456	25.31888462777172	32.295373906265645	16.91868002197618
21	24.449309996725223	24.7332140701668	33.65535290440092	17.16212302870706
22	23.930623479326965	22.779851200705835	34.23533996768528	19.054185352281923
23	21.98673310040028	24.796679756746443	34.2490074183133	18.967579724539977
24	21.595383920041357	24.85879223039262	35.05714618218035	18.488677667385677
25	21.853576947747037	25.699814068542942	33.46806823440896	18.978540749301064
26	21.201734277695532	26.612285549580367	32.90066605140585	19.285314121318244
27	20.507536042826484	25.750965517428032	34.120587510927194	19.620910928818294
28	19.945817354143944	26.581567615990647	34.295557943224594	19.17705708664081
29	21.06127077520156	24.765691180570027	34.681629593143	19.491408451085412
30	21.341791816309463	25.086943930975313	34.6055790262821	18.96568522643312
31	23.169035240371212	25.26367354008623	32.6075601300167	18.95973108952586
32	23.580817936025504	25.52727941952578	32.00226798487649	18.88963465957222
33	22.802179214108058	26.39184716271844	32.111201626020666	18.69477199715284
34	21.683613403303465	26.544895545493663	32.89836558941896	18.873125461783914
35	21.662773924128057	27.72882154098476	31.46883144650343	19.13957308838375
36	22.92356241424013	28.398120657878	29.70451242384794	18.97380450403393
37	22.679036837162474	28.661591216024206	29.731712003810646	18.927659943002674
38	22.546963254856003	28.148723514239858	29.536984662684613	19.767328568219526
39	22.20500634656866	28.92100213537001	28.895426410927467	19.978565107133868
40	22.91760827733287	27.510683616109727	29.554035145646306	20.01767296091109
41	20.830818612631973	27.928285127377933	29.973531155021366	21.26736510496873
42	21.616088077923415	28.19811578631144	29.040896801275267	21.14489933448988
43	21.531106305701627	27.429220197514958	30.01385690043871	21.0258165963447
44	21.48807413441735	28.00447101553218	29.747679916425568	20.759774933624904
45	21.389018947687493	28.220173157126965	29.307750391755143	21.083057503430393
46	21.693491857717778	28.356577020820534	29.289076053273288	20.6608550681884
47	21.257486650554412	27.881734602466636	30.51346311547507	20.34731563150388
48	21.720691437680482	27.19957996270545	30.598309566403515	20.481419033210553
49	21.654789967820594	27.45236013867726	30.408724434424656	20.484125459077486
50	20.70050420713901	28.125583573077556	30.727135437669663	20.446776782113773
51	20.669380309669247	28.335872862938476	30.300738042333915	20.694008785058365
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	582.0
1	930.0
2	1278.0
3	12581.0
4	23884.0
5	15892.0
6	7900.0
7	7680.0
8	7460.0
9	7951.0
10	8442.0
11	8388.5
12	8335.0
13	8463.0
14	8591.0
15	8063.0
16	7535.0
17	7185.5
18	6836.0
19	6333.5
20	5831.0
21	5752.5
22	5674.0
23	5701.0
24	5728.0
25	6104.5
26	7646.0
27	8811.0
28	10301.0
29	11791.0
30	13307.5
31	14824.0
32	16216.5
33	17609.0
34	20066.0
35	22523.0
36	25158.0
37	27793.0
38	28924.5
39	30056.0
40	32593.5
41	35131.0
42	38339.0
43	41547.0
44	43331.0
45	45115.0
46	45443.5
47	45772.0
48	45258.5
49	44745.0
50	43972.5
51	43200.0
52	40922.5
53	38645.0
54	37067.5
55	35490.0
56	34324.5
57	33159.0
58	32110.5
59	31062.0
60	29299.5
61	27537.0
62	25063.5
63	22590.0
64	20137.5
65	17685.0
66	15529.5
67	13374.0
68	11093.0
69	8812.0
70	7698.0
71	6584.0
72	5316.5
73	4049.0
74	3496.5
75	2352.5
76	1761.0
77	1357.5
78	954.0
79	714.5
80	475.0
81	343.5
82	212.0
83	144.0
84	76.0
85	66.0
86	56.0
87	39.0
88	22.0
89	15.5
90	9.0
91	8.5
92	8.0
93	5.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	738982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.39971477935738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.67837759835615	25.68551733687528
2	8.137763436667733	6.087000629465127
3	4.668401826847237	5.23790690398553
4	3.4721739944900216	5.1943326823291525
5	2.6703437217721593	4.993504677856321
6	2.2104555112685786	4.960224339234206
7	1.814700135010365	4.750848722161429
8	1.4776288156496167	4.421031700404425
9	1.2141822216228897	4.086906190097631
>10	5.621486362643113	30.986955004979126
>50	0.021780872236120446	0.5373633772130232
>100	0.011979474668335922	0.8186710554204675
>500	3.6301438388886367E-4	0.07317805951435481
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.6301438388886367E-4	2.1665593204639597
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15958	2.1594571992281275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.353212933467933E-4	0.0	0.0	0.018268374601817093	0.0
2	1.353212933467933E-4	0.0	0.0	0.05832347743246791	0.0
3	1.353212933467933E-4	0.0	0.0	0.09810793767642513	0.0
4	1.353212933467933E-4	0.0	0.0	0.15237177630848925	0.0
5	1.353212933467933E-4	0.0	0.0	0.3383032333669832	0.0
6	1.353212933467933E-4	0.0	0.0	0.6335742954496862	0.0
7	1.353212933467933E-4	0.0	0.0	0.8216708932017288	0.0
8	1.353212933467933E-4	0.0	0.0	1.1067928582834223	0.0
9	1.353212933467933E-4	0.0	0.0	1.2957013837955458	0.0
10	2.706425866935866E-4	0.0	0.0	1.5197934455778355	0.0
11	2.706425866935866E-4	0.0	0.0	1.6790666078470111	0.0
12	2.706425866935866E-4	0.0	0.0	1.8170943270607403	0.0
13	4.059638800403799E-4	0.0	0.0	1.9145256582704315	0.0
14	4.059638800403799E-4	0.0	0.0	1.992741365824878	0.0
15	4.059638800403799E-4	0.0	0.0	2.0520120923107736	0.0
16	4.059638800403799E-4	0.0	0.0	2.1154777788904195	0.0
17	4.059638800403799E-4	0.0	0.0	2.1827324616837758	0.0
18	4.059638800403799E-4	0.0	0.0	2.263789916398505	0.0
19	4.059638800403799E-4	0.0	0.0	2.3288794584983123	0.0
20	4.059638800403799E-4	0.0	0.0	2.399787816212032	0.0
21	4.059638800403799E-4	0.0	0.0	2.4794920579932933	0.0
22	4.059638800403799E-4	0.0	0.0	2.564203187628386	0.0
23	4.059638800403799E-4	0.0	0.0	2.658522129091101	0.0
24	4.059638800403799E-4	0.0	0.0	2.7320015913784097	0.0
25	4.059638800403799E-4	0.0	0.0	2.811029226692937	0.0
26	4.059638800403799E-4	0.0	0.0	2.887485757433875	0.0
27	4.059638800403799E-4	0.0	0.0	2.9704377102554593	0.0
28	4.059638800403799E-4	0.0	0.0	3.051359843676842	0.0
29	4.059638800403799E-4	0.0	0.0	3.144460893499436	0.0
30	4.059638800403799E-4	0.0	0.0	3.2539358198169914	0.0
31	4.059638800403799E-4	0.0	0.0	3.3546148620670055	0.0
32	4.059638800403799E-4	0.0	0.0	3.4685553910650055	0.0
33	4.059638800403799E-4	0.0	0.0	3.567745899088205	0.0
34	4.059638800403799E-4	0.0	0.0	3.671807973671889	0.0
35	4.059638800403799E-4	0.0	0.0	3.7838540045630342	0.0
36	4.059638800403799E-4	0.0	0.0	3.895223428987445	0.0
37	4.059638800403799E-4	0.0	0.0	4.007810745051977	0.0
38	4.059638800403799E-4	0.0	0.0	4.144755893918932	0.0
39	4.059638800403799E-4	0.0	0.0	4.326898354763715	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAA	25	3.8894486E-5	45.0	9
CGTTTTT	7865	0.0	43.254925	1
CGGGTAT	70	0.0	41.785717	6
CGTAAGG	65	0.0	41.538464	2
TACGCGG	60	3.6379788E-12	41.250004	2
TACGGGA	290	0.0	39.568966	4
ACGTTAG	40	3.455989E-7	39.375	1
AATACGG	40	3.455989E-7	39.375	2
TATCCGG	40	3.455989E-7	39.375	2
GGGCGAT	1375	0.0	39.10909	7
TTGGGCG	190	0.0	39.078945	5
TTAGGGA	1160	0.0	38.98707	4
ACGGGAT	405	0.0	38.88889	5
TAAGGGA	970	0.0	38.737114	4
ACACGAC	100	0.0	38.25	26
CGTTAGG	100	0.0	38.25	2
GTTTTTT	9220	0.0	38.142624	2
AGGGATC	820	0.0	38.140244	6
TGCGATG	30	1.1395272E-4	37.500004	1
GCGATAA	30	1.1395272E-4	37.500004	9
>>END_MODULE