FastQCFastQC Report
Thu 26 May 2016
SRR1547317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547317_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1633676
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292071.7878085985225958No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA24830.15198852159179665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT138400.043.1304171
TACGGGA5650.040.619474
TAGGGAC17650.038.2436265
TTAGGGA21200.037.5707554
ATAGGGA22800.037.54
TAGGGAT22550.037.3170745
GGGCGAT35250.037.0851067
TAAGGGA22900.037.0414854
TACGAAT1400.036.96428312
ACACGAC7150.036.8181826
ACGGGAC4950.036.818185
AGGGATC17100.036.5789456
GACCGAT9000.036.59
ACGGGAT6350.036.4960635
AGGGCGA18700.036.457226
GTTTTTT169950.036.354812
GACACGA7250.036.31034525
CACGACC7300.036.06164627
AGGGATT27650.036.0488246
CGAACCG250.002107367936.012
Produced by FastQC (version 0.11.5)