##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547317_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1633676
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.366079932618216	31.0	31.0	33.0	30.0	34.0
2	31.76960486657085	31.0	31.0	34.0	30.0	34.0
3	31.770912347368757	31.0	31.0	34.0	30.0	34.0
4	35.59988822753104	37.0	35.0	37.0	33.0	37.0
5	35.54314074516611	37.0	35.0	37.0	33.0	37.0
6	35.58081957499529	37.0	35.0	37.0	33.0	37.0
7	35.94727351078182	37.0	35.0	37.0	35.0	37.0
8	35.95108944490829	37.0	35.0	37.0	35.0	37.0
9	37.696263518592424	39.0	37.0	39.0	35.0	39.0
10	37.05946772799503	39.0	37.0	39.0	33.0	39.0
11	36.75988445689353	39.0	37.0	39.0	32.0	39.0
12	36.5421001471528	39.0	35.0	39.0	32.0	39.0
13	36.45953787654345	39.0	35.0	39.0	32.0	39.0
14	37.57314975552068	40.0	36.0	41.0	32.0	41.0
15	37.65377222900992	40.0	36.0	41.0	32.0	41.0
16	37.71807078025263	40.0	36.0	41.0	32.0	41.0
17	37.6078800202733	40.0	36.0	41.0	32.0	41.0
18	37.40119950345111	39.0	36.0	41.0	32.0	41.0
19	37.26354491343449	39.0	36.0	41.0	32.0	41.0
20	37.061392834319655	39.0	35.0	41.0	32.0	41.0
21	36.93015995827814	39.0	35.0	41.0	31.0	41.0
22	36.839799935850195	38.0	35.0	41.0	31.0	41.0
23	36.71648295010761	38.0	35.0	40.0	31.0	41.0
24	36.57408568161618	38.0	35.0	40.0	31.0	41.0
25	36.445322083448616	38.0	35.0	40.0	31.0	41.0
26	36.24722099118797	38.0	35.0	40.0	30.0	41.0
27	36.23497254045478	38.0	35.0	40.0	30.0	41.0
28	36.23018334112761	38.0	35.0	40.0	30.0	41.0
29	36.221701243086144	38.0	35.0	40.0	30.0	41.0
30	36.142735768904	38.0	35.0	40.0	30.0	41.0
31	36.02343793995872	38.0	35.0	40.0	30.0	41.0
32	35.76978421669903	38.0	35.0	40.0	29.0	41.0
33	35.466018965816964	38.0	34.0	40.0	27.0	41.0
34	35.108564366496175	38.0	34.0	40.0	24.0	41.0
35	34.84017271478555	38.0	34.0	40.0	23.0	41.0
36	34.564888631527914	38.0	33.0	40.0	23.0	41.0
37	34.50935007920787	38.0	33.0	40.0	22.0	41.0
38	34.39718157088676	38.0	33.0	40.0	22.0	41.0
39	34.243546455968016	38.0	33.0	40.0	21.0	41.0
40	34.130227168667474	37.0	33.0	40.0	21.0	41.0
41	34.0842884390785	37.0	33.0	40.0	21.0	41.0
42	33.98053102328736	37.0	33.0	40.0	20.0	41.0
43	33.86203690327825	37.0	33.0	40.0	20.0	41.0
44	33.7382057396938	37.0	33.0	40.0	19.0	41.0
45	33.58647614337237	36.0	33.0	40.0	20.0	41.0
46	33.50082084819756	36.0	33.0	40.0	19.0	41.0
47	33.4546831807531	36.0	33.0	40.0	19.0	41.0
48	33.33242882921705	36.0	32.0	40.0	18.0	41.0
49	33.20983597726844	36.0	32.0	40.0	18.0	41.0
50	33.09710187332127	35.0	32.0	40.0	18.0	41.0
51	32.64076046902813	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	1.0
12	3.0
13	11.0
14	20.0
15	51.0
16	181.0
17	416.0
18	946.0
19	1800.0
20	3138.0
21	4874.0
22	7528.0
23	11333.0
24	16342.0
25	24645.0
26	34415.0
27	39178.0
28	40480.0
29	41234.0
30	45136.0
31	52110.0
32	62117.0
33	78399.0
34	114789.0
35	144713.0
36	143605.0
37	171763.0
38	239544.0
39	354794.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.44976360061603	22.397525580347633	25.951963547239476	19.200747271796857
2	36.134582377411434	23.853873105805555	26.50911196589777	13.502432550885244
3	27.617593696669353	24.201371630604847	35.19002543956085	12.99100923316496
4	23.751343595670132	27.250874714447665	34.67064460762109	14.32713708226111
5	22.120114392327487	30.855995925752723	33.34014823012642	13.683741451793379
6	21.633298157039707	39.02303761578183	29.774692166623	9.568972060555458
7	85.11240907009713	2.597516276177161	10.950029259167668	1.3400453945580397
8	85.33681097108607	2.2672182244214887	11.119585523690132	1.2763852808023133
9	80.46216018353701	4.751921433625761	12.509395987943753	2.2765223948934796
10	37.46501754325827	34.564932091797886	17.60336810971086	10.366682255232984
11	27.130961096325095	26.096178189555335	30.351979217421327	16.420881496698243
12	25.55941324962844	23.133044740817642	32.781530731919915	18.526011277633998
13	23.480543265616927	25.018730764239667	33.37381463643953	18.12691133370387
14	20.859888986555475	26.45059363056077	33.58701480587338	19.102502577010373
15	19.946549989104327	24.74976678362172	36.79444394114867	18.50923928612528
16	23.60608835534096	24.305492643584163	34.37823656587965	17.71018243519523
17	23.402682049561847	24.16531797002588	32.576349288353384	19.85565069205889
18	23.37250470717572	24.312409559790314	33.598400172372	18.716685560661965
19	24.739911708319156	25.994689277433224	30.824716773705436	18.440682240542188
20	26.170489130035573	26.187812026374875	30.54112320925324	17.100575634336305
21	25.49728342706877	25.492508918537087	31.89206427712716	17.11814337726697
22	24.99192006248485	23.690805275954354	31.624997857592323	19.692276803968472
23	22.59530041452528	26.594747061228784	31.72752736772775	19.082425156518184
24	21.580533716599863	25.780326086690387	34.132349376498155	18.506790820211595
25	22.05645427857176	27.02518736885404	31.904367818343417	19.013990534230775
26	21.64358171387717	27.566971663903985	30.718086083164593	20.071360539054258
27	20.50290265634067	26.718210954926192	32.214343603015536	20.56454278571761
28	19.818678856762297	27.153303347787443	33.58768813399964	19.44032966145062
29	21.08306665458757	25.54429397261146	33.06487944978074	20.30775992302023
30	21.23542244606642	26.22178449092721	33.33335373721595	19.209439325790427
31	23.161324522120665	26.317703143095695	30.96134117168888	19.559631163094764
32	23.245674172847004	26.431495596434058	30.861321339114976	19.461508891603966
33	22.894319314233666	26.618252334000132	30.014090921333235	20.473337430432963
34	21.00765390444617	28.35513284151815	30.960055727084196	19.67715752695149
35	21.677186908542453	28.772106586618158	30.471097084121944	19.07960942071745
36	22.985279822926945	29.25280165712173	28.388982882774798	19.37293563717653
37	22.088957663575886	29.241844772158004	28.457968409892782	20.211229154373328
38	22.064166946199858	29.229051537759016	28.838827282765983	19.867954233275142
39	21.7864496999405	28.73060508938125	28.331688780394643	21.151256430283606
40	23.253264417179416	27.47943900748986	28.96571902874254	20.301577546588184
41	21.16313148996496	27.871132342031103	29.05404743657861	21.911688731425325
42	21.943947269838084	27.69478158459817	28.300593263290885	22.060677882272863
43	21.762577157282102	27.39894569057757	28.984755851221415	21.85372130091891
44	21.740112482524072	27.69239433033233	29.382264292307653	21.18522889483594
45	21.239217568232625	28.078456193272107	28.59600067577659	22.086325562718677
46	21.876247187324783	28.336769347165536	28.78612405397398	21.000859411535703
47	21.377861950594852	28.18551536534784	29.847901297442085	20.588721386615216
48	21.299817099596247	27.713451137189992	30.122925231196398	20.863806532017364
49	21.602386274879475	27.497374020307575	29.657716707596855	21.242522997216096
50	20.840423682541704	28.486921519322067	29.995298945445732	20.677355852690496
51	20.761093386938416	27.96337829532906	29.84526919658488	21.430259121147646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1348.0
1	2110.5
2	2873.0
3	22991.5
4	43110.0
5	28892.0
6	14674.0
7	14696.0
8	14718.0
9	15564.0
10	16410.0
11	17234.5
12	18059.0
13	17946.0
14	17833.0
15	16573.0
16	15313.0
17	14452.0
18	13591.0
19	12815.0
20	12039.0
21	11857.0
22	11675.0
23	11978.5
24	12282.0
25	13276.0
26	15380.0
27	16490.0
28	19858.0
29	23226.0
30	27759.5
31	32293.0
32	35405.0
33	38517.0
34	43463.5
35	48410.0
36	51694.5
37	54979.0
38	60556.0
39	66133.0
40	72229.5
41	78326.0
42	84451.0
43	90576.0
44	96231.0
45	101886.0
46	103567.5
47	105249.0
48	104694.5
49	104140.0
50	102375.5
51	100611.0
52	97843.5
53	95076.0
54	90375.0
55	85674.0
56	81276.5
57	76879.0
58	72561.5
59	68244.0
60	64288.5
61	60333.0
62	55440.5
63	50548.0
64	45367.5
65	40187.0
66	35531.5
67	30876.0
68	25732.5
69	20589.0
70	16680.5
71	12772.0
72	10869.5
73	8967.0
74	7627.5
75	5120.0
76	3952.0
77	3042.5
78	2133.0
79	1670.5
80	1208.0
81	862.5
82	517.0
83	373.0
84	229.0
85	171.0
86	113.0
87	69.5
88	26.0
89	21.5
90	17.0
91	14.0
92	11.0
93	7.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1633676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.769844615558352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.41443776978568	20.188327808668575
2	5.892359856716812	3.1547511556652545
3	2.656808586648966	2.1336705911362204
4	1.7056466017852034	1.8263957799538009
5	1.2756558016000008	1.7074553795883776
6	0.9889728940973498	1.588479042239106
7	0.8551335969864164	1.6024255461808905
8	0.7612654044473752	1.6303165270605133
9	0.7087669130377567	1.7076222117602735
>10	9.338290461788091	54.89489120324449
>50	0.375405326433383	6.105160682120173
>100	0.025639859232370384	1.2775230788706957
>500	0.0011549481718458121	0.22341190067579889
>1k	2.3098963436916245E-4	0.15353771087130047
>5k	0.0	0.0
>10k+	2.3098963436916245E-4	1.8060313819645883
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29207	1.7878085985225958	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	2483	0.15198852159179665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01915924577455995	0.0
2	0.0	0.0	0.0	0.05264201714415833	0.0
3	0.0	0.0	0.0	0.10020346751742695	0.0
4	0.0	0.0	0.0	0.153824871027058	0.0
5	0.0	0.0	0.0	0.2601495033286894	0.0
6	0.0	0.0	0.0	0.5278280393419503	0.0
7	0.0	0.0	0.0	0.6883861916316332	0.0
8	6.121164784204457E-5	0.0	0.0	0.9349467091393887	0.0
9	6.121164784204457E-5	0.0	0.0	1.0945254750635989	0.0
10	6.121164784204457E-5	0.0	0.0	1.2738143915929474	0.0
11	6.121164784204457E-5	0.0	0.0	1.4221302143142214	0.0
12	6.121164784204457E-5	0.0	0.0	1.5472468225033604	0.0
13	6.121164784204457E-5	0.0	0.0	1.6295764888509106	0.0
14	6.121164784204457E-5	0.0	0.0	1.6873602844138005	0.0
15	6.121164784204457E-5	0.0	0.0	1.730575707790284	0.0
16	6.121164784204457E-5	0.0	0.0	1.7815650104427072	0.0
17	6.121164784204457E-5	0.0	0.0	1.841736060271437	0.0
18	6.121164784204457E-5	0.0	0.0	1.910293105854527	0.0
19	6.121164784204457E-5	0.0	0.0	1.9610987735634238	0.0
20	6.121164784204457E-5	0.0	0.0	2.0156995634385275	0.0
21	6.121164784204457E-5	0.0	0.0	2.080645121798937	0.0
22	6.121164784204457E-5	0.0	0.0	2.1558130253489676	0.0
23	6.121164784204457E-5	0.0	0.0	2.237836633457307	0.0
24	6.121164784204457E-5	0.0	0.0	2.30737306540587	0.0
25	6.121164784204457E-5	0.0	0.0	2.3725634703576475	0.0
26	6.121164784204457E-5	0.0	0.0	2.4345096579737966	0.0
27	6.121164784204457E-5	0.0	0.0	2.5004346026996784	0.0
28	6.121164784204457E-5	0.0	0.0	2.569726188056873	0.0
29	6.121164784204457E-5	0.0	0.0	2.644037128537115	0.0
30	6.121164784204457E-5	0.0	0.0	2.7439345378153317	0.0
31	6.121164784204457E-5	0.0	0.0	2.826325415810724	0.0
32	6.121164784204457E-5	0.0	0.0	2.9256719202583623	0.0
33	6.121164784204457E-5	0.0	0.0	3.016265159064588	0.0
34	6.121164784204457E-5	0.0	0.0	3.1030020640567653	0.0
35	6.121164784204457E-5	0.0	0.0	3.1984922346903546	0.0
36	6.121164784204457E-5	0.0	0.0	3.2927581723671033	0.0
37	6.121164784204457E-5	0.0	0.0	3.3916761952798473	0.0
38	6.121164784204457E-5	0.0	0.0	3.5084680193624687	0.0
39	6.121164784204457E-5	0.0	0.0	3.6607013875456333	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13840	0.0	43.130417	1
TACGGGA	565	0.0	40.61947	4
TAGGGAC	1765	0.0	38.243626	5
TTAGGGA	2120	0.0	37.570755	4
ATAGGGA	2280	0.0	37.5	4
TAGGGAT	2255	0.0	37.317074	5
GGGCGAT	3525	0.0	37.085106	7
TAAGGGA	2290	0.0	37.041485	4
TACGAAT	140	0.0	36.964283	12
ACACGAC	715	0.0	36.81818	26
ACGGGAC	495	0.0	36.81818	5
AGGGATC	1710	0.0	36.578945	6
GACCGAT	900	0.0	36.5	9
ACGGGAT	635	0.0	36.496063	5
AGGGCGA	1870	0.0	36.45722	6
GTTTTTT	16995	0.0	36.35481	2
GACACGA	725	0.0	36.310345	25
CACGACC	730	0.0	36.061646	27
AGGGATT	2765	0.0	36.048824	6
CGAACCG	25	0.0021073679	36.0	12
>>END_MODULE