##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547316_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1832872
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25899571819527	31.0	31.0	33.0	30.0	34.0
2	31.639732070761077	31.0	31.0	34.0	30.0	34.0
3	31.640867992964047	31.0	31.0	34.0	30.0	34.0
4	35.455931456206436	37.0	35.0	37.0	33.0	37.0
5	35.367626326333756	37.0	35.0	37.0	33.0	37.0
6	35.41018958225124	37.0	35.0	37.0	33.0	37.0
7	35.88756716235503	37.0	35.0	37.0	35.0	37.0
8	35.90363156838011	37.0	35.0	37.0	35.0	37.0
9	37.654978634623696	39.0	37.0	39.0	35.0	39.0
10	36.89340063026769	39.0	37.0	39.0	32.0	39.0
11	36.5923626963585	39.0	35.0	39.0	32.0	39.0
12	35.98769035699165	37.0	35.0	39.0	31.0	39.0
13	35.63489976386785	37.0	35.0	39.0	30.0	39.0
14	36.7060138405737	39.0	35.0	41.0	31.0	41.0
15	36.97841147663339	39.0	35.0	41.0	31.0	41.0
16	37.13819841210952	39.0	35.0	41.0	32.0	41.0
17	37.06605153005775	38.0	35.0	41.0	32.0	41.0
18	36.91435899506349	38.0	35.0	40.0	32.0	41.0
19	36.762041211824936	38.0	35.0	40.0	31.0	41.0
20	36.520147069735366	38.0	35.0	40.0	31.0	41.0
21	36.378795682404444	38.0	35.0	40.0	31.0	41.0
22	36.27648193654549	38.0	35.0	40.0	31.0	41.0
23	36.20176149780236	37.0	35.0	40.0	31.0	41.0
24	36.084378505427544	37.0	35.0	40.0	31.0	41.0
25	36.001722433426885	37.0	35.0	40.0	30.0	41.0
26	35.74958807816367	37.0	34.0	40.0	30.0	41.0
27	35.67785748268292	37.0	34.0	40.0	30.0	41.0
28	35.708801269264846	37.0	34.0	40.0	30.0	41.0
29	35.71694913774666	37.0	34.0	40.0	30.0	41.0
30	35.63797744741586	37.0	34.0	40.0	30.0	41.0
31	35.481254010099995	37.0	34.0	40.0	29.0	41.0
32	35.19813767682631	37.0	34.0	40.0	27.0	41.0
33	34.89236946169727	37.0	34.0	40.0	25.0	41.0
34	34.608363813730584	37.0	34.0	40.0	24.0	41.0
35	34.38064305636182	37.0	33.0	40.0	23.0	41.0
36	34.07617225861926	36.0	33.0	40.0	22.0	41.0
37	34.00350651873126	36.0	33.0	40.0	22.0	41.0
38	33.965912513257884	36.0	33.0	40.0	22.0	41.0
39	33.85528558459074	36.0	33.0	40.0	21.0	41.0
40	33.71496536583024	36.0	33.0	40.0	20.0	41.0
41	33.744870345556045	36.0	33.0	40.0	21.0	41.0
42	33.65008467585298	36.0	33.0	40.0	20.0	41.0
43	33.52158797777477	36.0	33.0	40.0	19.0	41.0
44	33.412205544086	35.0	33.0	40.0	18.0	41.0
45	33.23611523336054	35.0	32.0	40.0	18.0	41.0
46	33.15601689588798	35.0	32.0	40.0	18.0	41.0
47	33.12149511804425	35.0	32.0	40.0	18.0	41.0
48	33.021830220550044	35.0	32.0	40.0	18.0	41.0
49	32.8875229694163	35.0	32.0	39.0	18.0	41.0
50	32.72501680422855	35.0	32.0	39.0	18.0	41.0
51	32.29699073366825	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	2.0
13	5.0
14	21.0
15	69.0
16	181.0
17	535.0
18	1266.0
19	2430.0
20	4127.0
21	6578.0
22	9920.0
23	14243.0
24	21062.0
25	30579.0
26	41055.0
27	46226.0
28	46888.0
29	49756.0
30	55470.0
31	64927.0
32	78060.0
33	98419.0
34	156746.0
35	225876.0
36	141629.0
37	160347.0
38	225650.0
39	350688.0
40	114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.572020304745777	24.105720421284193	27.840842131911014	17.48141714205902
2	32.9739338044337	25.100388897860842	28.961706000200778	12.963971297504681
3	26.729144206469407	24.571819526950055	35.28516994094514	13.413866325635396
4	22.999151059102875	27.434321654758214	35.13889676965986	14.427630516479056
5	21.234325146545967	31.440384271242074	34.08994190538128	13.23534867683068
6	19.704976670493085	39.998756050613466	30.41630839469423	9.879958884199223
7	85.2548350348524	3.1165842459266115	10.475254136677302	1.15332658254368
8	85.77707554046327	2.4683665853371104	10.041563186081733	1.7129946881178828
9	81.60171577720648	4.616416203641062	11.734043621158488	2.047824397993968
10	40.81823498858622	29.575824171027765	18.20187116176143	11.404069678624584
11	34.2325050521804	22.313669476100895	28.54525575162914	14.908569720089565
12	31.78437992396632	21.760930386846436	31.04242958591762	15.412260103269624
13	19.639833005250775	34.954759524942276	31.04854021448306	14.356867255323886
14	15.469874601172368	36.69066907018057	31.34697894888459	16.49247737976247
15	14.455401140941648	26.06766866426024	44.496942503349935	14.979987691448176
16	15.697713751969586	22.751670602202445	44.144326499613726	17.406289146214245
17	15.820908388583598	24.46308307399535	31.50029025485686	28.215718282564197
18	19.269976299490637	24.98259562042521	36.56649236826139	19.18093571182276
19	23.326833515924736	27.614039605602574	29.404726571195372	19.654400307277324
20	25.80703944410739	25.453714170984114	30.30631708051626	18.432929304392232
21	19.89729779275367	30.272654064222703	32.11359003792955	17.716458105094084
22	22.113055357930065	25.372530105757523	29.710858150487322	22.80355638582509
23	17.3404907707685	30.294859651956056	29.6606637015569	22.703985875718544
24	17.337817370771116	26.688224818754392	39.3825100716253	16.591447738849194
25	16.388214779864608	28.36433749874514	35.86737098935441	19.380076732035842
26	17.41152682784177	33.782118991397105	30.305826047863683	18.500528132897443
27	16.784478130496836	34.254110488893936	31.28898253669651	17.672428843912723
28	15.863901025276178	29.865042403397506	37.15895054319123	17.11210602813508
29	16.223227808597652	25.975136288840684	36.87404248632747	20.927593416234195
30	17.262798493293584	31.898081262630452	33.66672631804076	17.172393926035205
31	23.855020972550182	28.989749420581468	29.180924799986034	17.974304806882312
32	24.202617531393354	28.541218372041254	30.427711264070812	16.828452832494577
33	23.41489203828745	29.75210489330406	28.99596916751415	17.837033900894333
34	19.133851136358675	29.365498518172572	30.290549476450074	21.210100869018678
35	18.721983859211118	28.861699016625273	31.647490932263683	20.768826191899926
36	25.04582971424082	28.722191184108876	28.77718684119786	17.454792260452447
37	18.31278998206094	32.566976853811944	31.355162826427595	17.765070337699523
38	18.91692382228546	33.707863942490256	28.03938300110428	19.335829234120002
39	18.277271953524306	32.85843201271011	29.990855880825286	18.873440152940304
40	21.58252185640896	29.879446027873197	28.241361098865603	20.29667101685224
41	17.06442130165118	27.74983741363281	30.396994443692737	24.78874684102327
42	19.470590417661462	28.817833432994778	28.669814367833652	23.04176178151011
43	19.6009323073297	29.664919317879264	29.027067902177567	21.70708047261347
44	18.298059002483534	31.858634972873173	29.105960481692122	20.73734554295117
45	17.011116979254414	35.95963056885587	26.136194998886992	20.89305745300272
46	20.139704245577434	33.53458397531306	27.59979965867775	18.725912120431758
47	19.32857286269854	30.071385235848442	29.131330502075432	21.46871139937759
48	20.001778629386013	28.957668620612896	30.679174541375502	20.361378208625588
49	19.798873025503145	27.777989952380743	31.79932914027821	20.6238078818379
50	18.387481504436753	32.64390530271618	29.97694328900218	18.991669903844898
51	17.218387317826885	33.17973104504843	27.619713760699057	21.98216787642563
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2511.0
1	2799.0
2	3087.0
3	20881.0
4	38675.0
5	26282.5
6	13890.0
7	14404.0
8	14918.0
9	16881.5
10	18845.0
11	20076.5
12	21308.0
13	21227.0
14	21146.0
15	19882.5
16	18619.0
17	17324.0
18	16029.0
19	15934.5
20	15840.0
21	15881.0
22	15922.0
23	16278.5
24	16635.0
25	16964.0
26	19718.5
27	22144.0
28	26164.5
29	30185.0
30	33843.5
31	37502.0
32	43071.0
33	48640.0
34	55128.5
35	61617.0
36	65548.5
37	69480.0
38	74472.5
39	79465.0
40	98909.5
41	118354.0
42	136023.5
43	153693.0
44	165693.0
45	177693.0
46	170405.0
47	163117.0
48	150796.0
49	138475.0
50	130319.0
51	122163.0
52	111263.0
53	100363.0
54	88197.5
55	76032.0
56	67966.5
57	59901.0
58	50918.5
59	41936.0
60	37535.5
61	33135.0
62	27936.5
63	22738.0
64	19227.5
65	15717.0
66	12292.5
67	8868.0
68	7571.5
69	6275.0
70	5310.5
71	4346.0
72	3445.5
73	2545.0
74	2145.0
75	1425.0
76	1105.0
77	784.0
78	463.0
79	319.0
80	175.0
81	162.0
82	149.0
83	110.5
84	72.0
85	51.0
86	30.0
87	26.5
88	23.0
89	14.0
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1832872.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.89984340498367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0448921012806	20.99050014196965
2	7.722825241789745	4.0003992881281905
3	2.720665073430579	2.1139439807778118
4	1.4767779527903493	1.5299327088480965
5	0.9651807079272259	1.2499014596413216
6	0.6691170527530917	1.0398016131545562
7	0.4953182839932511	0.8980066193735302
8	0.37458626450812155	0.7761380473934512
9	0.30525379770936134	0.711542300350412
>10	2.5299682484369477	14.217187638169465
>50	0.8337971425170068	15.805073478382006
>100	0.8577887189899382	34.22214955166147
>500	0.0027656877977125676	0.45904374500760675
>1k	8.509808608346362E-4	0.47221280695176926
>5k	0.0	0.0
>10k+	2.1274521520865905E-4	1.5141666201907373
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27480	1.49928636587825	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4112	0.2243473630455373	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1935	0.10557202030474577	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0911836724004731E-4	0.0	0.0	0.07300018768359165	0.0
2	1.0911836724004731E-4	0.0	0.0	0.24900811404178796	0.0
3	1.0911836724004731E-4	0.0	0.0	0.35419822006119356	0.0
4	1.0911836724004731E-4	0.0	0.0	0.5523026157854994	0.0
5	1.0911836724004731E-4	0.0	0.0	0.9207407827715193	0.0
6	1.0911836724004731E-4	0.0	0.0	1.4495283904167886	0.0
7	1.0911836724004731E-4	0.0	0.0	1.7154498513807839	0.0
8	1.0911836724004731E-4	0.0	0.0	2.315928226302764	0.0
9	1.0911836724004731E-4	0.0	0.0	2.517797205696852	0.0
10	1.0911836724004731E-4	0.0	0.0	2.8904909890052335	0.0
11	1.0911836724004731E-4	0.0	0.0	3.342513825297129	0.0
12	1.0911836724004731E-4	0.0	0.0	3.679143988232675	0.0
13	1.0911836724004731E-4	0.0	0.0	3.8501324696978294	0.0
14	1.0911836724004731E-4	0.0	0.0	3.9283703390089433	0.0
15	1.6367755086007098E-4	0.0	0.0	4.028922914420647	0.0
16	1.6367755086007098E-4	0.0	0.0	4.261345036641948	0.0
17	1.6367755086007098E-4	0.0	0.0	4.545980297587612	0.0
18	1.6367755086007098E-4	0.0	0.0	4.8728989258387925	0.0
19	1.6367755086007098E-4	0.0	0.0	5.056599697087413	0.0
20	1.6367755086007098E-4	0.0	0.0	5.229934223448228	0.0
21	1.6367755086007098E-4	0.0	0.0	5.493891553801902	0.0
22	1.6367755086007098E-4	0.0	0.0	5.769197194348542	0.0
23	1.6367755086007098E-4	0.0	0.0	6.109646500137489	0.0
24	1.6367755086007098E-4	0.0	0.0	6.3504161774526535	0.0
25	1.6367755086007098E-4	0.0	0.0	6.562433165000066	0.0
26	1.6367755086007098E-4	0.0	0.0	6.760592119907991	0.0
27	1.6367755086007098E-4	0.0	0.0	6.957332536041797	0.0
28	1.6367755086007098E-4	0.0	0.0	7.163402572574626	0.0
29	1.6367755086007098E-4	0.0	0.0	7.385131094806402	0.0
30	1.6367755086007098E-4	0.0	0.0	7.660436735353041	0.0
31	1.6367755086007098E-4	0.0	0.0	7.91053603306723	0.0
32	1.6367755086007098E-4	0.0	0.0	8.143940220593691	0.0
33	1.6367755086007098E-4	0.0	0.0	8.377780881589112	0.0
34	1.6367755086007098E-4	0.0	0.0	8.612276252787975	0.0
35	1.6367755086007098E-4	0.0	0.0	8.899312117812919	0.0
36	1.6367755086007098E-4	0.0	0.0	9.137462954314323	0.0
37	1.6367755086007098E-4	0.0	0.0	9.392854492839653	0.0
38	2.1823673448009463E-4	0.0	0.0	9.655120488501106	0.0
39	2.1823673448009463E-4	0.0	0.0	9.938391769856269	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGAG	65	0.0	45.000004	1
CGTATTG	20	7.0343877E-4	45.0	13
GCGCATC	20	7.0343877E-4	45.0	28
ACTTACG	40	6.8193913E-9	45.0	1
CCTCGAC	20	7.0343877E-4	45.0	22
GTTACGT	25	3.891717E-5	45.0	19
CGCTAAT	25	3.891717E-5	45.0	35
CGGCTCG	20	7.0343877E-4	45.0	20
ACCCGTG	20	7.0343877E-4	45.0	38
GCTCGAT	25	3.891717E-5	45.0	32
TAATGCG	55	1.8189894E-12	45.0	1
TCGAATA	25	3.891717E-5	45.0	21
TACGTAC	35	1.2124656E-7	45.0	13
CGTTTTT	14295	0.0	43.740818	1
CGGTCTA	260	0.0	43.269234	31
CGACGGT	245	0.0	42.2449	28
TTAGCGG	370	0.0	41.351353	2
GCTACGA	180	0.0	41.250004	10
CGTAAGG	225	0.0	41.0	2
TCGTCCC	600	0.0	40.875	38
>>END_MODULE