FastQCFastQC Report
Thu 26 May 2016
SRR1547315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547315_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1939054
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165700.8545404099112247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA253.8918002E-545.00000431
ATATCGT351.212502E-745.031
CGTTTTT82350.041.256831
TACGGGA4650.040.1612934
GGGCGAT36650.039.290597
AATAGCG1550.039.193551
CATACGA3450.039.13043218
GCGATAT1450.038.7931029
CGTTAGG2750.038.4545442
CGTAAGG3400.038.382352
AGGGCGA20000.038.36256
TCGTTGA2850.037.89473724
TCACGAC1850.037.702725
AAGGGAT27600.037.581525
TATCCGA301.140211E-437.50000423
TAAGGGA21550.037.378194
TAACGGG4400.037.3295443
TAGCGGG9150.036.8852463
GGCGATA6650.036.87978
AGGGATT23700.036.7405056
Produced by FastQC (version 0.11.5)