##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547315_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1939054
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27149011837731	31.0	31.0	33.0	30.0	34.0
2	31.67485691476359	31.0	31.0	34.0	30.0	34.0
3	31.764276291428708	31.0	31.0	34.0	30.0	34.0
4	35.56827298259873	37.0	35.0	37.0	33.0	37.0
5	35.46070764403673	37.0	35.0	37.0	33.0	37.0
6	35.491399414353594	37.0	35.0	37.0	33.0	37.0
7	35.87738402334334	37.0	35.0	37.0	35.0	37.0
8	35.86610842194183	37.0	35.0	37.0	35.0	37.0
9	37.59049670612577	39.0	37.0	39.0	35.0	39.0
10	36.887075347050676	39.0	37.0	39.0	32.0	39.0
11	36.604564390677105	39.0	35.0	39.0	32.0	39.0
12	36.154014277065	38.0	35.0	39.0	31.0	39.0
13	35.96200105824799	38.0	35.0	39.0	31.0	39.0
14	37.02894297941161	39.0	35.0	41.0	31.0	41.0
15	37.198804674856916	39.0	35.0	41.0	32.0	41.0
16	37.290891847261605	39.0	35.0	41.0	32.0	41.0
17	37.20067156458768	39.0	35.0	41.0	32.0	41.0
18	37.05065511326657	39.0	35.0	41.0	31.0	41.0
19	36.984151550188905	39.0	35.0	41.0	31.0	41.0
20	36.838498566826914	38.0	35.0	41.0	31.0	41.0
21	36.68925362573709	38.0	35.0	41.0	31.0	41.0
22	36.595558452730046	38.0	35.0	40.0	31.0	41.0
23	36.50799668291858	38.0	35.0	40.0	31.0	41.0
24	36.38428223247006	38.0	35.0	40.0	30.0	41.0
25	36.25399086358606	38.0	35.0	40.0	30.0	41.0
26	36.009982702905646	38.0	35.0	40.0	30.0	41.0
27	35.992227137562956	38.0	35.0	40.0	30.0	41.0
28	36.034254332267174	38.0	35.0	40.0	30.0	41.0
29	36.03520067001744	38.0	35.0	40.0	30.0	41.0
30	36.011055906643136	38.0	35.0	40.0	30.0	41.0
31	35.91880886246592	38.0	35.0	40.0	30.0	41.0
32	35.74088240966987	38.0	34.0	40.0	29.0	41.0
33	35.553607068188924	38.0	34.0	40.0	27.0	41.0
34	35.339927614187125	38.0	34.0	40.0	27.0	41.0
35	35.21045210705839	38.0	34.0	40.0	26.0	41.0
36	34.9801655858991	38.0	34.0	40.0	25.0	41.0
37	34.93752314272836	38.0	34.0	40.0	25.0	41.0
38	34.85469718739138	38.0	34.0	40.0	24.0	41.0
39	34.69833279527027	37.0	33.0	40.0	24.0	41.0
40	34.57244357300003	37.0	33.0	40.0	23.0	41.0
41	34.5395166921602	37.0	33.0	40.0	23.0	41.0
42	34.44324448932314	37.0	33.0	40.0	23.0	41.0
43	34.324436039429536	37.0	33.0	40.0	23.0	41.0
44	34.223094354257285	37.0	33.0	40.0	23.0	41.0
45	34.06331747336588	36.0	33.0	40.0	23.0	41.0
46	33.95917803217445	36.0	33.0	40.0	23.0	41.0
47	33.927857089075395	36.0	33.0	40.0	23.0	41.0
48	33.79110947915839	36.0	33.0	40.0	23.0	41.0
49	33.658859423203275	36.0	33.0	40.0	22.0	41.0
50	33.53617537211444	35.0	32.0	40.0	22.0	41.0
51	33.06066669623435	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	2.0
12	5.0
13	16.0
14	26.0
15	92.0
16	244.0
17	540.0
18	1238.0
19	2306.0
20	3934.0
21	5947.0
22	9093.0
23	12986.0
24	17982.0
25	24761.0
26	32523.0
27	38953.0
28	42221.0
29	48239.0
30	55630.0
31	66419.0
32	79816.0
33	101064.0
34	154267.0
35	195686.0
36	163063.0
37	200151.0
38	275775.0
39	405966.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.198067717557116	24.030068270404023	26.751962554936583	15.019901457102277
2	32.88077588349783	25.659316347043454	27.608565826428766	13.851341943029952
3	28.719571502392405	25.890820987966297	31.25622081695507	14.133386692686228
4	25.248136462419303	28.724934942502887	31.00032283783742	15.026605757240386
5	22.89162653541366	33.21506260269183	29.90839863149763	13.984912230396883
6	22.773269852206283	41.47800937983161	26.228563000308398	9.52015776765371
7	88.86596247448499	2.9523674946649243	6.882428235624176	1.2992417952259194
8	88.98684616312903	2.660730438657201	6.797696196186388	1.5547272020273804
9	83.86357471220502	5.168963834942193	8.424468838928673	2.5429926139241092
10	41.58924919058469	30.83513919674233	15.200711274673115	12.37490033799987
11	34.2260710635186	24.682035673065318	24.111035587456563	16.980857675959516
12	31.20779514134212	22.046678431853884	28.10442617895118	18.64110024785282
13	24.68244824538151	26.958093998413656	29.72160651534202	18.637851240862812
14	21.11065498949488	28.549746422740164	30.72255852596163	19.617040061803333
15	20.25028699561745	24.621232827966626	36.438335394475864	18.69014478194006
16	23.36799284599604	22.84810015605548	34.3604149239784	19.423492073970092
17	23.892939546809938	23.659268901227094	28.77960077439824	23.668190777564728
18	24.336609501334156	24.63644643212618	31.046840366487988	19.980103700051675
19	27.366953163759234	24.915654747108643	27.56859788329773	20.148794205834392
20	28.364037308914554	25.353497117666656	27.192125644773174	19.090339928645616
21	26.278484250567548	26.03857344870231	29.2175462880353	18.46539601269485
22	25.974779454311225	23.65571046499994	28.437475181196604	21.932034899492226
23	24.279674521699757	26.036613730200397	28.40457253898035	21.2791392091195
24	23.338287639230266	24.746345382851636	31.909838508881133	20.005528469036964
25	22.766152979751983	25.81913654802806	29.970439193544895	21.444271278675064
26	21.8858268000788	28.556192865180652	27.956828432833742	21.601151901906807
27	21.457731450490805	27.774007325221472	29.585045078682697	21.183216145605023
28	20.629234668039157	27.399340090580253	31.500824628916984	20.470600612463606
29	21.709916278762737	25.49129627127455	31.404179563849176	21.394607886113537
30	22.585962020655433	26.907605461219745	29.827328171366034	20.67910434675878
31	25.409606952668675	26.493279712684636	27.265563517055224	20.831549817591466
32	26.31520318670857	26.149452258678718	27.475717540615165	20.059627013997545
33	25.441168734857307	26.622621133810608	27.19645765409318	20.739752477238902
34	22.97806043565574	26.74206081934799	28.64964049479798	21.630238250198293
35	22.320781164423476	27.812221836008693	28.663874239706576	21.20312275986125
36	25.4629319245364	27.376287612413066	26.34026695491719	20.82051350813335
37	23.24030171413483	29.753323012149224	26.800903945944775	20.20547132777117
38	22.911636292748938	29.900353471331897	25.92738520948875	21.260625026430414
39	22.794414183411085	29.01729400006395	26.572906169709558	21.615385646815405
40	24.30999858693982	27.08965299573916	26.69559486223695	21.904753555084078
41	21.317560006064813	26.360379855331516	28.299469741430617	24.022590397173055
42	22.925302750722775	27.020856562014263	26.761193860511362	23.2926468267516
43	22.09964240294494	26.90182944879307	27.67241139235937	23.32611675590262
44	21.703727694019868	27.401609238319303	27.871271248763573	23.023391818897256
45	21.702541548610817	28.723078367080028	26.723495065119383	22.85088501918977
46	22.09525882208541	29.17020361475235	27.097491869746793	21.63704569341545
47	22.25755445696716	27.92609179527749	27.87797554890168	21.938378198853666
48	22.59065503075211	26.87490910516159	28.898576316079904	21.6358595480064
49	22.47276249140045	26.55392784316476	28.766914175675357	22.20639548975944
50	21.676807350388387	28.760313018616294	28.020158283369106	21.542721347626216
51	20.984717289977482	28.55913244293351	27.560088579276286	22.896061687812715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1788.0
1	2736.0
2	3684.0
3	14872.0
4	26060.0
5	17719.0
6	9378.0
7	9564.0
8	9750.0
9	10731.5
10	11713.0
11	12309.5
12	12906.0
13	12731.0
14	12556.0
15	11854.5
16	11153.0
17	10507.0
18	9861.0
19	9500.0
20	9139.0
21	9438.0
22	9737.0
23	10449.0
24	11161.0
25	12901.0
26	17531.0
27	20421.0
28	21623.5
29	22826.0
30	27265.5
31	31705.0
32	36465.0
33	41225.0
34	46096.0
35	50967.0
36	57295.5
37	63624.0
38	69949.5
39	76275.0
40	84489.5
41	92704.0
42	105220.0
43	117736.0
44	124894.0
45	132052.0
46	134479.0
47	136906.0
48	136085.5
49	135265.0
50	128955.0
51	122645.0
52	118070.0
53	113495.0
54	110062.0
55	106629.0
56	104162.0
57	101695.0
58	97382.5
59	93070.0
60	88047.5
61	83025.0
62	76103.5
63	69182.0
64	62121.0
65	55060.0
66	46772.0
67	38484.0
68	33348.5
69	28213.0
70	23830.0
71	19447.0
72	16347.5
73	13248.0
74	11228.0
75	7158.5
76	5109.0
77	3830.0
78	2551.0
79	1880.0
80	1209.0
81	1001.5
82	794.0
83	587.0
84	380.0
85	305.0
86	230.0
87	152.5
88	75.0
89	62.5
90	50.0
91	30.0
92	10.0
93	8.0
94	6.0
95	4.5
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1939054.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.98135354093768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30534786888278	17.652202427338988
2	5.697318652368242	2.504695510661699
3	2.0118551885955367	1.3266990052106504
4	1.1461250032803156	1.0077351559685188
5	0.7544571418407406	0.8291994583143343
6	0.5562033048437883	0.7335660890645548
7	0.4674602830849443	0.719278682418588
8	0.4043341232426562	0.7110249049329513
9	0.3402066194937652	0.6730381782053741
>10	5.971200947199702	33.68853500732556
>50	2.078322940428208	31.685969741362165
>100	0.26503626786727574	7.256756858541215
>500	0.0014211059147255145	0.22314224323429438
>1k	4.737019715751715E-4	0.12547195664830832
>5k	0.0	0.0
>10k+	2.3685098578758574E-4	0.8626847807728086
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16570	0.8545404099112247	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.062861580956487E-4	0.0	0.0	0.013614886434312815	0.0
2	2.062861580956487E-4	0.0	0.0	0.05172625414248391	0.0
3	2.062861580956487E-4	0.0	0.0	0.07947174240634866	0.0
4	2.062861580956487E-4	0.0	0.0	0.12980556498168694	0.0
5	2.062861580956487E-4	0.0	0.0	0.22516134156140055	0.0
6	2.062861580956487E-4	0.0	0.0	0.416337038576543	0.0
7	2.062861580956487E-4	0.0	0.0	0.5123632451700675	0.0
8	2.5785769761956087E-4	0.0	0.0	0.7402063067867115	0.0
9	2.5785769761956087E-4	0.0	0.0	0.8516524036978856	0.0
10	2.5785769761956087E-4	0.0	0.0	1.014051181658685	0.0
11	2.5785769761956087E-4	0.0	0.0	1.1802146820047301	0.0
12	2.5785769761956087E-4	0.0	0.0	1.314661685543569	0.0
13	2.5785769761956087E-4	0.0	0.0	1.386346125481807	0.0
14	2.5785769761956087E-4	0.0	0.0	1.4243543501109304	0.0
15	2.5785769761956087E-4	0.0	0.0	1.4611248577914797	0.0
16	2.5785769761956087E-4	0.0	0.0	1.5359035901011524	0.0
17	2.5785769761956087E-4	0.0	0.0	1.6110948947270163	0.0
18	2.5785769761956087E-4	0.0	0.0	1.7053676689767279	0.0
19	2.5785769761956087E-4	0.0	0.0	1.7679239464192333	0.0
20	2.5785769761956087E-4	0.0	0.0	1.83563737781413	0.0
21	2.5785769761956087E-4	0.0	0.0	1.929652294366222	0.0
22	2.5785769761956087E-4	0.0	0.0	2.0294947948845157	0.0
23	2.5785769761956087E-4	0.0	0.0	2.1419723225861684	0.0
24	2.5785769761956087E-4	0.0	0.0	2.2288187951444365	0.0
25	2.5785769761956087E-4	0.0	0.0	2.307723250616022	0.0
26	2.5785769761956087E-4	0.0	0.0	2.3854415606785575	0.0
27	2.5785769761956087E-4	0.0	0.0	2.4685233108515803	0.0
28	2.5785769761956087E-4	0.0	0.0	2.550883059471268	0.0
29	2.5785769761956087E-4	0.0	0.0	2.645980978353362	0.0
30	2.5785769761956087E-4	0.0	0.0	2.759954080701208	0.0
31	2.5785769761956087E-4	0.0	0.0	2.8595902950614063	0.0
32	2.5785769761956087E-4	0.0	0.0	2.963145946425422	0.0
33	2.5785769761956087E-4	0.0	0.0	3.06304001848324	0.0
34	2.5785769761956087E-4	0.0	0.0	3.1705666783905966	0.0
35	2.5785769761956087E-4	0.0	0.0	3.28252849069701	0.0
36	2.5785769761956087E-4	0.0	0.0	3.387579716707219	0.0
37	2.5785769761956087E-4	0.0	0.0	3.500469816725063	0.0
38	2.5785769761956087E-4	0.0	0.0	3.629450237074367	0.0
39	2.5785769761956087E-4	0.0	0.0	3.7964904535923187	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	25	3.8918002E-5	45.000004	31
ATATCGT	35	1.212502E-7	45.0	31
CGTTTTT	8235	0.0	41.25683	1
TACGGGA	465	0.0	40.161293	4
GGGCGAT	3665	0.0	39.29059	7
AATAGCG	155	0.0	39.19355	1
CATACGA	345	0.0	39.130432	18
GCGATAT	145	0.0	38.793102	9
CGTTAGG	275	0.0	38.454544	2
CGTAAGG	340	0.0	38.38235	2
AGGGCGA	2000	0.0	38.3625	6
TCGTTGA	285	0.0	37.894737	24
TCACGAC	185	0.0	37.7027	25
AAGGGAT	2760	0.0	37.58152	5
TATCCGA	30	1.140211E-4	37.500004	23
TAAGGGA	2155	0.0	37.37819	4
TAACGGG	440	0.0	37.329544	3
TAGCGGG	915	0.0	36.885246	3
GGCGATA	665	0.0	36.8797	8
AGGGATT	2370	0.0	36.740505	6
>>END_MODULE