##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547314_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2122465
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.348338370715183	31.0	31.0	33.0	30.0	34.0
2	31.746143752664945	31.0	31.0	34.0	30.0	34.0
3	31.816752219706803	31.0	31.0	34.0	30.0	34.0
4	35.60132581691571	37.0	35.0	37.0	33.0	37.0
5	35.51220679728523	37.0	35.0	37.0	33.0	37.0
6	35.54811174742575	37.0	35.0	37.0	33.0	37.0
7	35.96642017654001	37.0	35.0	37.0	35.0	37.0
8	35.9751553029143	37.0	35.0	37.0	35.0	37.0
9	37.73109050090343	39.0	38.0	39.0	35.0	39.0
10	37.046113834621536	39.0	37.0	39.0	33.0	39.0
11	36.65517217009468	39.0	35.0	39.0	32.0	39.0
12	36.299786804493834	38.0	35.0	39.0	32.0	39.0
13	36.11044657980226	38.0	35.0	39.0	31.0	39.0
14	37.238312999272075	39.0	35.0	41.0	32.0	41.0
15	37.41373403094986	39.0	35.0	41.0	32.0	41.0
16	37.53212373348913	39.0	35.0	41.0	32.0	41.0
17	37.44093495063523	39.0	35.0	41.0	32.0	41.0
18	37.310040919402674	39.0	36.0	41.0	32.0	41.0
19	37.23741687142073	39.0	35.0	41.0	32.0	41.0
20	37.08567019950859	39.0	35.0	41.0	32.0	41.0
21	36.95936564324971	39.0	35.0	41.0	32.0	41.0
22	36.842167479793545	38.0	35.0	41.0	31.0	41.0
23	36.74377999166064	38.0	35.0	40.0	31.0	41.0
24	36.6696247052366	38.0	35.0	40.0	31.0	41.0
25	36.587185654415975	38.0	35.0	40.0	31.0	41.0
26	36.37681610768611	38.0	35.0	40.0	31.0	41.0
27	36.357320850991655	38.0	35.0	40.0	30.0	41.0
28	36.3948531542334	38.0	35.0	40.0	30.0	41.0
29	36.42478721675033	38.0	35.0	40.0	30.0	41.0
30	36.40426343897308	38.0	35.0	40.0	30.0	41.0
31	36.29525622330639	38.0	35.0	40.0	30.0	41.0
32	36.10101367984867	38.0	35.0	40.0	30.0	41.0
33	35.91917699467365	38.0	35.0	40.0	29.0	41.0
34	35.74617343513321	38.0	35.0	40.0	29.0	41.0
35	35.60837893675514	38.0	35.0	40.0	28.0	41.0
36	35.37615461267912	38.0	34.0	40.0	26.0	41.0
37	35.339283804444364	38.0	34.0	40.0	26.0	41.0
38	35.291580308744784	38.0	34.0	40.0	26.0	41.0
39	35.19054589828336	38.0	34.0	40.0	25.0	41.0
40	35.07472867632682	38.0	34.0	40.0	25.0	41.0
41	35.0580655040248	38.0	34.0	40.0	25.0	41.0
42	34.98325202064581	38.0	34.0	40.0	25.0	41.0
43	34.87612186773398	38.0	34.0	40.0	24.0	41.0
44	34.77668983940843	38.0	34.0	40.0	24.0	41.0
45	34.621838758236294	37.0	34.0	40.0	23.0	41.0
46	34.54999352168351	37.0	34.0	40.0	23.0	41.0
47	34.539268256484796	37.0	33.0	40.0	23.0	41.0
48	34.453257886466915	37.0	33.0	40.0	23.0	41.0
49	34.334218467677914	37.0	33.0	40.0	24.0	41.0
50	34.223725715147246	37.0	33.0	40.0	23.0	41.0
51	33.74710819730832	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	5.0
14	13.0
15	48.0
16	155.0
17	410.0
18	899.0
19	1816.0
20	3033.0
21	4896.0
22	7426.0
23	11452.0
24	16696.0
25	24205.0
26	32688.0
27	38854.0
28	42437.0
29	47065.0
30	54977.0
31	66182.0
32	81108.0
33	103862.0
34	160566.0
35	222870.0
36	165675.0
37	204933.0
38	307826.0
39	522169.0
40	195.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.70249450520974	23.906589743529338	28.563344978598	14.827570772662918
2	31.699462653094397	26.6498151912988	28.908179875757668	12.742542279849136
3	27.65218743300832	25.958779061138813	33.038848697151664	13.350184808701204
4	24.652938917720668	28.21125436697425	32.66970244503443	14.466104270270652
5	21.944013211054127	33.39517966138429	30.730589196995002	13.930217930566583
6	20.491315522281877	41.856803292398226	27.977516708167155	9.674364477152745
7	89.40877705875009	2.89846946828334	6.791301623348324	0.9014518496182504
8	89.9724141505278	2.262086771748886	6.585880096962729	1.179618980760578
9	85.44739253650826	5.240369099137088	7.6275462728478445	1.6846920915068093
10	40.80251028874445	37.60104406904236	12.78014007298118	8.816305569232002
11	28.449609298622118	25.049364771621676	31.046825271559246	15.454200658196953
12	29.906029074684387	23.208816164224146	30.27663589270023	16.608518868391233
13	21.116013691627426	31.377478544993675	30.912924359176714	16.59358340420219
14	17.926608919346137	33.65082580867058	30.473011333520223	17.949553938463062
15	16.02382135865609	27.759374123954927	40.14337103320903	16.07343348417995
16	19.31278018718801	24.728181619013743	39.36093174681326	16.598106446984993
17	19.99180198495617	24.38885918024561	30.81850584108572	24.8008329937125
18	19.876275933878766	26.131832562609986	34.821540048952514	19.17035145455873
19	23.922655968414084	26.972270449689397	30.155079117912425	18.949994463984094
20	26.016871891880434	26.16825248001734	29.819054731173424	17.99582089692881
21	22.82374503230913	27.859964710843286	31.12159682256245	18.194693434285135
22	22.675992301404264	25.4872518510317	29.96266133952739	21.874094508036645
23	18.856800936646774	29.580982489699476	30.805643438172126	20.75657313548162
24	19.314711903376498	26.59176947558617	36.77431665539832	17.31920196563901
25	18.467300992006937	27.886867392395164	34.11161079216854	19.53422082342936
26	18.78561012784663	31.968772158787072	30.724841163458528	18.520776549907772
27	18.463578904716922	32.56548400091403	30.325588407818266	18.64534868655078
28	17.039056003279207	30.078752770952644	35.19115745135962	17.69103377440853
29	17.305208802029714	26.78479974934805	35.45033722581998	20.45965422280226
30	19.279092941461933	29.68449420838506	32.7970543683877	18.239358481765304
31	23.417347282522915	28.168662380769533	29.506305168754253	18.9076851679533
32	23.169663575135516	28.57319201965639	30.339770031543512	17.917374373664583
33	22.67015003780981	28.862384067581797	29.179750902841743	19.28771499176665
34	19.84452040434118	28.82162956750759	29.8991973954812	21.43465263267003
35	19.35725677455223	27.9901906509648	31.715481762950155	20.937070811532816
36	23.4600806138146	28.14449237089893	29.58696609838089	18.808460916905577
37	19.665765984362523	30.80243961620097	30.496050582695123	19.035743816741384
38	19.249787393431696	31.134977490794903	29.153413601637716	20.461821514135686
39	19.910198754749782	30.550421326146722	30.032108892254993	19.5072710268485
40	21.77251450553955	28.07707076441779	29.90970404694541	20.240710683097248
41	18.13702463880441	28.236979172801433	31.197263559116408	22.42873262927775
42	19.971071372201664	28.817907480217574	28.790957683636716	22.420063463944047
43	20.943384225417145	28.601461037048903	28.90893371622147	21.546221021312483
44	19.952319590664626	30.56318949900234	29.942637452207695	19.541853458125342
45	18.815810861427632	32.25655075584285	28.3194304735296	20.608207909199916
46	21.120772309555164	30.831321128970323	28.45601694256442	19.591889618910088
47	20.390583590306555	28.804385466898157	29.926571227322945	20.87845971547234
48	20.954691832374152	27.427967010056705	31.478540282171906	20.13880087539724
49	19.930929367504294	27.499817429262674	31.406030252560114	21.163222950672917
50	18.873715232053296	30.65445131015117	30.50570916363756	19.96612429415797
51	18.218439408894845	30.943831818192525	29.2293630283656	21.608365744547026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2345.0
1	3005.0
2	3665.0
3	17157.0
4	30649.0
5	21023.0
6	11397.0
7	11727.5
8	12058.0
9	13012.5
10	13967.0
11	14619.5
12	15272.0
13	15225.0
14	15178.0
15	14747.5
16	14317.0
17	13657.0
18	12997.0
19	12779.5
20	12562.0
21	12932.0
22	13302.0
23	14623.5
24	15945.0
25	18526.0
26	26480.0
27	31853.0
28	37999.0
29	44145.0
30	50477.5
31	56810.0
32	64646.5
33	72483.0
34	79779.0
35	87075.0
36	90086.0
37	93097.0
38	102965.5
39	112834.0
40	125911.0
41	138988.0
42	153104.5
43	167221.0
44	171472.0
45	175723.0
46	167749.0
47	159775.0
48	157946.5
49	156118.0
50	148350.5
51	140583.0
52	132634.0
53	124685.0
54	112992.5
55	101300.0
56	87012.0
57	72724.0
58	66583.0
59	60442.0
60	54725.0
61	49008.0
62	40637.0
63	32266.0
64	26227.5
65	20189.0
66	15493.5
67	10798.0
68	9057.0
69	7316.0
70	5569.0
71	3822.0
72	3442.0
73	3062.0
74	2799.0
75	2102.5
76	1669.0
77	1113.0
78	557.0
79	432.5
80	308.0
81	272.0
82	236.0
83	140.5
84	45.0
85	33.5
86	22.0
87	14.0
88	6.0
89	5.5
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2122465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.29275386918882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.00378031911244	15.820787500396666
2	6.459854209805813	2.4925675460125354
3	2.124548314135304	1.2296516312343742
4	1.085981895031762	0.8380632562897212
5	0.6978814286224733	0.6732027316145621
6	0.4946504205067947	0.5725901288477003
7	0.38221151846261453	0.5161738926167698
8	0.29295549896924267	0.45215346689911984
9	0.2455738656730018	0.42640165324210144
>10	3.343823393964435	16.144423668129413
>50	1.5625887802245952	22.012327614913502
>100	1.301729387764149	37.20957858691437
>500	0.003438530454584799	0.41210561635952137
>1k	7.368279545538855E-4	0.2570151619103189
>5k	0.0	0.0
>10k+	2.4560931818462853E-4	0.9429575446193488
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19900	0.9375890768516795	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2793	0.1315922759621478	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.711502898752158E-5	0.0	0.0	0.023416169406798228	0.0
2	4.711502898752158E-5	0.0	0.0	0.08791664409071527	0.0
3	4.711502898752158E-5	0.0	0.0	0.12758749849820844	0.0
4	4.711502898752158E-5	0.0	0.0	0.22247716687907693	0.0
5	4.711502898752158E-5	0.0	0.0	0.40000659610405825	0.0
6	4.711502898752158E-5	0.0	0.0	0.6641334486081043	0.0
7	4.711502898752158E-5	0.0	0.0	0.7874805944974358	0.0
8	4.711502898752158E-5	0.0	0.0	1.0769553325967685	0.0
9	4.711502898752158E-5	0.0	0.0	1.1906910125726455	0.0
10	4.711502898752158E-5	0.0	0.0	1.3770780672472809	0.0
11	4.711502898752158E-5	0.0	0.0	1.6027119410685218	0.0
12	4.711502898752158E-5	0.0	0.0	1.7716664350177742	0.0
13	4.711502898752158E-5	0.0	0.0	1.8604782646592524	0.0
14	4.711502898752158E-5	0.0	0.0	1.9037769762987846	0.0
15	4.711502898752158E-5	0.0	0.0	1.9484891388079426	0.0
16	4.711502898752158E-5	0.0	0.0	2.0356519424348574	0.0
17	4.711502898752158E-5	0.0	0.0	2.1434040137293193	0.0
18	4.711502898752158E-5	0.0	0.0	2.2724991931551286	0.0
19	4.711502898752158E-5	0.0	0.0	2.3518880169991023	0.0
20	4.711502898752158E-5	0.0	0.0	2.427931673784962	0.0
21	4.711502898752158E-5	0.0	0.0	2.5349299046156237	0.0
22	4.711502898752158E-5	0.0	0.0	2.653282857432278	0.0
23	4.711502898752158E-5	0.0	0.0	2.792036617800529	0.0
24	4.711502898752158E-5	0.0	0.0	2.8941348856164884	0.0
25	4.711502898752158E-5	0.0	0.0	2.986056307171143	0.0
26	4.711502898752158E-5	0.0	0.0	3.076187357624272	0.0
27	4.711502898752158E-5	0.0	0.0	3.163020356048274	0.0
28	4.711502898752158E-5	0.0	0.0	3.25814559957408	0.0
29	4.711502898752158E-5	0.0	0.0	3.368677457578806	0.0
30	4.711502898752158E-5	0.0	0.0	3.498950512729303	0.0
31	4.711502898752158E-5	0.0	0.0	3.6208370927200213	0.0
32	4.711502898752158E-5	0.0	0.0	3.742299637449852	0.0
33	4.711502898752158E-5	0.0	0.0	3.8542920613531906	0.0
34	4.711502898752158E-5	0.0	0.0	3.970571952894394	0.0
35	4.711502898752158E-5	0.0	0.0	4.105320935798706	0.0
36	4.711502898752158E-5	0.0	0.0	4.237525707137691	0.0
37	4.711502898752158E-5	0.0	0.0	4.372463150157953	0.0
38	4.711502898752158E-5	0.0	0.0	4.513337086830643	0.0
39	4.711502898752158E-5	0.0	0.0	4.647662034474067	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	35	1.2125747E-7	45.000004	41
CGACTCG	35	1.2125747E-7	45.000004	29
CGCGATA	20	7.034638E-4	45.0	11
TAAGTCG	20	7.034638E-4	45.0	19
CGTTCGA	20	7.034638E-4	45.0	14
ACCTACG	20	7.034638E-4	45.0	34
CTACGGT	25	3.8919257E-5	45.0	33
GTGTCGA	20	7.034638E-4	45.0	29
CCGCGTA	25	3.8919257E-5	45.0	19
CGCGTGT	20	7.034638E-4	45.0	21
CGGTACG	20	7.034638E-4	45.0	23
GTGCGTA	25	3.8919257E-5	45.0	23
CTTACGT	20	7.034638E-4	45.0	35
AGTACGG	255	0.0	44.11765	2
CGTTTTT	10410	0.0	42.406338	1
CGAACGG	75	0.0	42.000004	2
TAACGCG	120	0.0	41.250004	1
GTATGCG	165	0.0	40.90909	1
CGTAAGG	535	0.0	40.79439	2
GCGTAAG	260	0.0	40.673077	1
>>END_MODULE