FastQCFastQC Report
Thu 26 May 2016
SRR1547313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547313_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2560223
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312521.2206749177708347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT154050.042.473221
CGTTAGG3450.040.4347842
CGGTCTA950.040.26315731
TAAGGGA31850.038.924654
AGTACGG3250.038.769232
AGGGCGA26450.037.939516
TACGGGA8500.037.852944
GGGCGAT49750.037.537697
GCGATCG2350.037.3404279
TGGGCGA15550.037.331196
TAGGGAT29700.037.2727285
TAGCATA9750.037.1538530
ACGGGTA2250.037.0000045
ATAGGGT12450.036.686754
TACGTAG1350.036.6666681
ACGGGAT9950.036.6331675
TTAGGGA28700.036.53314
AAGGGAT34350.036.288215
ATTAGCG2750.036.01
ACGTTAC250.002107590436.028
Produced by FastQC (version 0.11.5)