FastQCFastQC Report
Thu 26 May 2016
SRR1547312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547312_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1865031
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT457182.4513265463147795No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28530.15297332859346574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22650.12144570251111109No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT19200.1029473504729948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG207.034417E-445.0000041
CGACGCC207.034417E-445.00000427
ACGTTAT207.034417E-445.00000411
CGTGCGC207.034417E-445.00000412
CGCGACT207.034417E-445.00000439
CCGTAGA207.034417E-445.00000428
CTCGCGA207.034417E-445.00000437
ACGTCCG207.034417E-445.00000425
CGTAAAT207.034417E-445.00000432
CCGCGTG207.034417E-445.0000041
ACCGTAC207.034417E-445.00000430
CACGTCG207.034417E-445.00000425
CACGTAC406.8193913E-945.00000428
ATACCCG207.034417E-445.00000413
TCGCGAC207.034417E-445.00000438
TACCGCG207.034417E-445.0000041
CGTGCTA253.8917442E-545.020
CCTAGCG253.8917442E-545.043
TAGCGCA253.8917442E-545.045
TAGCGAT351.2124656E-745.017