##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547312_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1865031
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301252365242185	31.0	31.0	33.0	30.0	34.0
2	31.68332108152626	31.0	31.0	34.0	30.0	34.0
3	31.67272125771636	31.0	31.0	34.0	30.0	34.0
4	35.48409007678693	37.0	35.0	37.0	33.0	37.0
5	35.41306015824938	37.0	35.0	37.0	33.0	37.0
6	35.45989798560989	37.0	35.0	37.0	33.0	37.0
7	35.91933699761559	37.0	35.0	37.0	35.0	37.0
8	35.93721927410322	37.0	35.0	37.0	35.0	37.0
9	37.69900178602929	39.0	37.0	39.0	35.0	39.0
10	36.96268373019001	39.0	37.0	39.0	32.0	39.0
11	36.68703737364151	39.0	35.0	39.0	32.0	39.0
12	36.12079799209772	38.0	35.0	39.0	32.0	39.0
13	35.74995750740872	38.0	35.0	39.0	30.0	39.0
14	36.85194455212809	39.0	35.0	41.0	31.0	41.0
15	37.09951845304448	39.0	35.0	41.0	32.0	41.0
16	37.25998924414661	39.0	35.0	41.0	32.0	41.0
17	37.19651523218649	39.0	35.0	41.0	32.0	41.0
18	37.02884080747183	38.0	35.0	40.0	32.0	41.0
19	36.87939288944795	38.0	35.0	40.0	32.0	41.0
20	36.632244182536375	38.0	35.0	40.0	32.0	41.0
21	36.46810696444188	38.0	35.0	40.0	31.0	41.0
22	36.3578975362876	38.0	35.0	40.0	31.0	41.0
23	36.297982714496435	38.0	35.0	40.0	31.0	41.0
24	36.1501090330402	37.0	35.0	40.0	31.0	41.0
25	36.04107867375931	37.0	35.0	40.0	31.0	41.0
26	35.81927539006054	37.0	35.0	40.0	30.0	41.0
27	35.73295778997775	37.0	34.0	40.0	30.0	41.0
28	35.72919538602844	37.0	34.0	40.0	30.0	41.0
29	35.71508999046129	37.0	35.0	40.0	30.0	41.0
30	35.63597334307044	36.0	35.0	40.0	30.0	41.0
31	35.46417834341628	36.0	34.0	40.0	29.0	41.0
32	35.161063274551466	36.0	34.0	40.0	27.0	41.0
33	34.88078750433639	36.0	34.0	40.0	26.0	41.0
34	34.62077466808863	36.0	34.0	40.0	24.0	41.0
35	34.41879840067001	36.0	33.0	40.0	23.0	41.0
36	34.09959727210968	36.0	33.0	40.0	22.0	41.0
37	34.021583555447606	36.0	33.0	40.0	22.0	41.0
38	33.95708543182392	35.0	33.0	40.0	22.0	41.0
39	33.85151935812327	36.0	33.0	40.0	21.0	41.0
40	33.74613022518125	35.0	33.0	40.0	21.0	41.0
41	33.738978065243955	35.0	33.0	40.0	21.0	41.0
42	33.64067567777694	35.0	33.0	40.0	21.0	41.0
43	33.518648751682946	35.0	33.0	40.0	20.0	41.0
44	33.41221513208092	35.0	33.0	40.0	19.0	41.0
45	33.26793924605006	35.0	33.0	40.0	20.0	41.0
46	33.19023651617587	35.0	33.0	40.0	20.0	41.0
47	33.15865205457711	35.0	33.0	40.0	19.0	41.0
48	33.05527736536283	35.0	32.0	40.0	18.0	41.0
49	32.95585971493235	35.0	32.0	39.0	18.0	41.0
50	32.812383815604136	35.0	32.0	39.0	18.0	41.0
51	32.37485918464626	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	3.0
14	25.0
15	66.0
16	213.0
17	514.0
18	1142.0
19	2140.0
20	3887.0
21	6380.0
22	9862.0
23	14704.0
24	21513.0
25	30335.0
26	40053.0
27	44693.0
28	45129.0
29	47450.0
30	54113.0
31	64117.0
32	78087.0
33	99610.0
34	160091.0
35	241519.0
36	165718.0
37	163115.0
38	218370.0
39	352045.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.242339135381663	23.723519877149496	28.107843783829868	17.926297203638974
2	33.523893168531785	25.76273531110207	28.061571094528727	12.651800425837425
3	26.738483167303922	24.52232697472589	36.26127394129106	12.477915916679134
4	22.577372708550154	27.649084653284582	35.61136517301857	14.16217746514669
5	20.511026358275007	31.317281053237185	35.02606659085023	13.145625997637572
6	19.281770651533407	38.988413597414734	32.22091214569624	9.508903605355622
7	84.04396495286137	3.5364559623941907	11.136865821533261	1.2827132632111744
8	84.56299117816273	2.8799521294820303	10.614408017882813	1.9426486744724352
9	80.58525568743897	4.974126435431905	12.118189992552402	2.3224278845767174
10	40.167267997153935	28.23550922209872	18.785478632794845	12.811744147952501
11	33.73488161858972	26.88314564208316	26.66604469309089	12.71592804623623
12	32.95232089975984	24.800981860355137	27.825006662087652	14.421690577797367
13	18.97812958604978	38.258667014113975	28.30542763096163	14.45777576887462
14	15.132617098589781	39.61135230459976	28.88842062142667	16.367609975383786
15	13.500258172652357	29.572001752249694	42.07989036107175	14.847849714026202
16	14.591231995607579	26.6260453579592	42.50122384024716	16.28149880618606
17	15.218352938905575	26.9442170130148	30.098427318366287	27.73900272971334
18	17.71691730593218	29.600044181571246	34.736419930821526	17.94661858167505
19	22.652063156054776	31.459852409959943	27.67616195119545	18.21192248278983
20	25.363170907078757	28.876570952439934	28.430465767056955	17.329792373424354
21	19.110298970901823	34.051176629235655	29.68921160023614	17.149312799626387
22	20.34909875492686	30.153171716716777	27.546244539634994	21.95148498872137
23	17.155907864266062	34.617011727955195	28.010419129762454	20.21666127801629
24	16.4009606274641	29.924381953972883	38.34568969631068	15.328967722252337
25	15.133099664295123	31.878880297432055	34.50478839225728	18.483231646015536
26	15.49974236353176	37.44039643308878	29.03619296408478	18.023668239294683
27	15.348699297759664	37.96076311868274	29.320585019766426	17.369952563791166
28	14.29000375865066	33.55783362314085	36.02578187708408	16.12638074112441
29	13.8647025170091	30.038321078845335	35.77136251354535	20.32561389060021
30	15.638131484141551	36.31387360317335	31.78772899753409	16.26026591515101
31	22.708308869932996	33.16261231046562	26.855961107348886	17.273117712252503
32	22.95983283923967	32.611843985435094	27.905380661232975	16.52294251409226
33	21.537711705596315	34.05434011552623	26.30996482096008	18.097983357917375
34	16.432595490369863	32.994733063418245	28.389501300514574	22.18317014569731
35	16.61141289340499	32.7560775129207	30.133869088503083	20.498640505171227
36	23.699498828705796	32.38664665627542	26.29371844221356	17.620136072805224
37	16.813929634413583	37.15348431205701	28.686869011828758	17.34571704170065
38	17.236013771352862	36.28207788503248	27.479918564356304	19.00198977925836
39	16.680312552445507	36.80378503091906	27.973047096804287	18.542855319831144
40	21.400073242750388	32.69725811528066	26.636983513946955	19.265685128022
41	15.825956780343061	30.453971006380055	29.219246221644575	24.50082599163231
42	18.60778721640552	31.296852438377698	27.044697916549374	23.050662428667408
43	18.982794387868086	32.72937554389176	26.877354853619057	21.410475214621098
44	17.310543363622376	35.07314355632695	27.940125392017613	19.676187688033068
45	15.942255115330523	38.93913827705813	24.467475339551996	20.65113126805935
46	18.853734870894908	37.16313562616386	26.238384241334327	17.744745261606912
47	18.34559318316961	32.96218668751351	28.139746738794152	20.55247339052273
48	19.086653251340056	30.68259991388883	30.652895313804436	19.577851520966675
49	18.016054424832618	31.043505443073066	30.5002973140929	20.440142818001416
50	17.08813419187134	34.786392290530294	29.140641630085508	18.98483188751286
51	15.7085324587098	35.77441876301252	27.048129494898475	21.468919283379204
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2987.0
1	3184.0
2	3381.0
3	28978.0
4	54575.0
5	34078.5
6	13582.0
7	15204.5
8	16827.0
9	21061.5
10	25296.0
11	28953.0
12	32610.0
13	33019.5
14	33429.0
15	30930.0
16	28431.0
17	25905.5
18	23380.0
19	21363.0
20	19346.0
21	17898.0
22	16450.0
23	16014.0
24	15578.0
25	16117.0
26	18698.0
27	20740.0
28	24427.0
29	28114.0
30	33533.5
31	38953.0
32	42338.5
33	45724.0
34	54409.0
35	63094.0
36	63111.0
37	63128.0
38	72327.0
39	81526.0
40	99838.5
41	118151.0
42	141287.5
43	164424.0
44	173124.0
45	181824.0
46	173244.5
47	164665.0
48	157607.0
49	150549.0
50	133480.5
51	116412.0
52	103098.0
53	89784.0
54	81124.5
55	72465.0
56	61133.5
57	49802.0
58	43734.5
59	37667.0
60	32219.0
61	26771.0
62	22632.5
63	18494.0
64	15680.0
65	12866.0
66	9625.0
67	6384.0
68	5087.5
69	3791.0
70	3205.0
71	2619.0
72	2324.5
73	2030.0
74	1507.5
75	819.0
76	653.0
77	571.0
78	489.0
79	320.5
80	152.0
81	134.0
82	116.0
83	90.0
84	64.0
85	45.0
86	26.0
87	20.0
88	14.0
89	11.5
90	9.0
91	9.0
92	9.0
93	7.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1865031.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.475694762757456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.78704354039792	19.52843323717618
2	7.682121860774941	3.760505395892675
3	2.948275876987804	2.164833013246636
4	1.610045675929226	1.5762794607256496
5	1.1220566836217332	1.3731558447418726
6	0.8219302188728659	1.2070387892051215
7	0.6040624920111973	1.0349394420467677
8	0.47248165578273355	0.9251453430352337
9	0.36580097788454274	0.8057909770758234
>10	2.877610902942387	14.987168109707591
>50	0.8054735256368535	14.364484980192996
>100	0.8960256103082532	34.35209288861336
>500	0.0055242022262349776	0.8598190855313835
>1k	0.0013258085342963946	0.5877251228122748
>5k	0.0	0.0
>10k+	2.209680890493991E-4	2.4725883099964645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45718	2.4513265463147795	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2853	0.15297332859346574	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2265	0.12144570251111109	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1920	0.1029473504729948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.36184117046848E-5	0.0	0.0	0.08932827390000488	0.0
2	5.36184117046848E-5	0.0	0.0	0.3042308680123816	0.0
3	5.36184117046848E-5	0.0	0.0	0.42996604345986744	0.0
4	5.36184117046848E-5	0.0	0.0	0.6676028441350305	0.0
5	5.36184117046848E-5	0.0	0.0	1.122394212214167	0.0
6	5.36184117046848E-5	0.0	0.0	1.7687105469024376	0.0
7	5.36184117046848E-5	0.0	0.0	2.180607185617826	0.0
8	5.36184117046848E-5	0.0	0.0	2.9207021223775906	0.0
9	5.36184117046848E-5	0.0	0.0	3.2452007500143427	0.0
10	5.36184117046848E-5	0.0	0.0	3.71259244484408	0.0
11	5.36184117046848E-5	0.0	0.0	4.286309450084207	0.0
12	5.36184117046848E-5	0.0	0.0	4.682442275758419	0.0
13	5.36184117046848E-5	0.0	0.0	4.866675138375716	0.0
14	5.36184117046848E-5	0.0	0.0	4.946137624522059	0.0
15	5.36184117046848E-5	0.0	0.0	5.046833001703457	0.0
16	5.36184117046848E-5	0.0	0.0	5.261306648522196	0.0
17	5.36184117046848E-5	0.0	0.0	5.51931844564514	0.0
18	5.36184117046848E-5	0.0	0.0	5.838884179405061	0.0
19	5.36184117046848E-5	0.0	0.0	6.0017769141638935	0.0
20	5.36184117046848E-5	0.0	0.0	6.167296951096255	0.0
21	5.36184117046848E-5	0.0	0.0	6.4126011846451885	0.0
22	5.36184117046848E-5	0.0	0.0	6.650345222143761	0.0
23	5.36184117046848E-5	0.0	0.0	6.932485304533812	0.0
24	5.36184117046848E-5	0.0	0.0	7.1465300040589135	0.0
25	5.36184117046848E-5	0.0	0.0	7.329797735265527	0.0
26	5.36184117046848E-5	0.0	0.0	7.503843099658933	0.0
27	5.36184117046848E-5	0.0	0.0	7.665234518890035	0.0
28	5.36184117046848E-5	0.0	0.0	7.835526594464113	0.0
29	5.36184117046848E-5	0.0	0.0	8.021260772609141	0.0
30	5.36184117046848E-5	0.0	0.0	8.25803967869703	0.0
31	5.36184117046848E-5	0.0	0.0	8.461682406351423	0.0
32	5.36184117046848E-5	0.0	0.0	8.650365597140208	0.0
33	5.36184117046848E-5	0.0	0.0	8.835188262286257	0.0
34	5.36184117046848E-5	0.0	0.0	9.034434280180866	0.0
35	5.36184117046848E-5	0.0	0.0	9.280489171493665	0.0
36	5.36184117046848E-5	0.0	0.0	9.480807557622366	0.0
37	5.36184117046848E-5	0.0	0.0	9.69442330985383	0.0
38	5.36184117046848E-5	0.0	0.0	9.896618340392198	0.0
39	5.36184117046848E-5	0.0	0.0	10.098438042048631	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.034417E-4	45.000004	1
CGACGCC	20	7.034417E-4	45.000004	27
ACGTTAT	20	7.034417E-4	45.000004	11
CGTGCGC	20	7.034417E-4	45.000004	12
CGCGACT	20	7.034417E-4	45.000004	39
CCGTAGA	20	7.034417E-4	45.000004	28
CTCGCGA	20	7.034417E-4	45.000004	37
ACGTCCG	20	7.034417E-4	45.000004	25
CGTAAAT	20	7.034417E-4	45.000004	32
CCGCGTG	20	7.034417E-4	45.000004	1
ACCGTAC	20	7.034417E-4	45.000004	30
CACGTCG	20	7.034417E-4	45.000004	25
CACGTAC	40	6.8193913E-9	45.000004	28
ATACCCG	20	7.034417E-4	45.000004	13
TCGCGAC	20	7.034417E-4	45.000004	38
TACCGCG	20	7.034417E-4	45.000004	1
CGTGCTA	25	3.8917442E-5	45.0	20
CCTAGCG	25	3.8917442E-5	45.0	43
TAGCGCA	25	3.8917442E-5	45.0	45
TAGCGAT	35	1.2124656E-7	45.0	17
>>END_MODULE