FastQCFastQC Report
Thu 26 May 2016
SRR1547311_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547311_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences706556
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51460.7283216050815505No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG207.03144E-445.01
TTAACGG406.8084773E-945.02
TAGGCGT207.03144E-445.014
CGGGTAC302.16431E-644.9999966
CGGTCTA1200.044.99999631
CACGACC1400.043.3928627
TACGGGA2550.041.470594
TATTGCG501.0804797E-940.4999961
CGTTTTT25400.040.4822851
ACAACGA950.040.26315713
TAGGGCG2750.040.0909085
GCGATAT451.9264917E-840.09
AGGGCGA6850.039.4160586
GGGCGAT13050.039.1379287
TTAGGGA9100.039.0659374
CTAGGGA4250.038.647064
ACACGTC356.2448707E-638.5714341
TAATGCG356.2448707E-638.571431
TAGGGAC6200.038.4677435
TAACGGG1650.038.1818163
Produced by FastQC (version 0.11.5)