##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547311_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	706556
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.267811751651674	31.0	31.0	33.0	30.0	34.0
2	31.67273931577964	31.0	31.0	34.0	30.0	34.0
3	31.780102355651923	31.0	31.0	34.0	30.0	34.0
4	35.574359286454296	37.0	35.0	37.0	33.0	37.0
5	35.45786887380476	37.0	35.0	37.0	33.0	37.0
6	35.486169532209765	37.0	35.0	37.0	33.0	37.0
7	35.86959561591721	37.0	35.0	37.0	35.0	37.0
8	35.86201801414184	37.0	35.0	37.0	35.0	37.0
9	37.60305764865064	39.0	37.0	39.0	35.0	39.0
10	36.89246429157774	39.0	37.0	39.0	32.0	39.0
11	36.62304474096887	39.0	35.0	39.0	32.0	39.0
12	36.173973471317204	38.0	35.0	39.0	32.0	39.0
13	35.958517088525184	38.0	35.0	39.0	31.0	39.0
14	36.979809102180155	39.0	35.0	41.0	31.0	41.0
15	37.15715527148591	39.0	35.0	41.0	32.0	41.0
16	37.26831985008973	39.0	35.0	41.0	32.0	41.0
17	37.17703196915743	39.0	35.0	41.0	32.0	41.0
18	37.08645174621686	39.0	35.0	41.0	32.0	41.0
19	37.033047628213474	39.0	35.0	41.0	31.0	41.0
20	36.89919553439501	39.0	35.0	41.0	31.0	41.0
21	36.764683903328255	38.0	35.0	41.0	31.0	41.0
22	36.63828486347862	38.0	35.0	40.0	31.0	41.0
23	36.557334450489414	38.0	35.0	40.0	31.0	41.0
24	36.44173993285741	38.0	35.0	40.0	30.0	41.0
25	36.32111396690425	38.0	35.0	40.0	30.0	41.0
26	36.10538159749546	38.0	35.0	40.0	30.0	41.0
27	36.090314143535686	38.0	35.0	40.0	30.0	41.0
28	36.14701311714853	38.0	35.0	40.0	30.0	41.0
29	36.13743284325658	38.0	35.0	40.0	30.0	41.0
30	36.11767644744366	38.0	35.0	40.0	30.0	41.0
31	36.039202837425485	38.0	35.0	40.0	30.0	41.0
32	35.90291640011549	38.0	35.0	40.0	29.0	41.0
33	35.76989368146332	38.0	34.0	40.0	29.0	41.0
34	35.60970680314087	38.0	34.0	40.0	28.0	41.0
35	35.494606230787085	38.0	34.0	40.0	27.0	41.0
36	35.26851799432741	38.0	34.0	40.0	26.0	41.0
37	35.23569823198727	38.0	34.0	40.0	26.0	41.0
38	35.15434586925877	38.0	34.0	40.0	26.0	41.0
39	35.03709260129416	38.0	34.0	40.0	26.0	41.0
40	34.9068481479175	37.0	34.0	40.0	25.0	41.0
41	34.87372267732494	37.0	34.0	40.0	25.0	41.0
42	34.76281993217806	37.0	33.0	40.0	25.0	41.0
43	34.66765125481915	37.0	33.0	40.0	24.0	41.0
44	34.559706520077675	37.0	33.0	40.0	24.0	41.0
45	34.40430482509525	37.0	33.0	40.0	24.0	41.0
46	34.319401434564284	36.0	33.0	40.0	23.0	41.0
47	34.280157552975275	36.0	33.0	40.0	23.0	41.0
48	34.16223767118247	36.0	33.0	40.0	23.0	41.0
49	34.022667700790876	36.0	33.0	40.0	24.0	41.0
50	33.88914396028057	36.0	33.0	40.0	23.0	41.0
51	33.39331914243174	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	3.0
14	6.0
15	32.0
16	71.0
17	174.0
18	396.0
19	676.0
20	1226.0
21	1895.0
22	2863.0
23	4002.0
24	5664.0
25	7962.0
26	10433.0
27	12440.0
28	14386.0
29	16732.0
30	19979.0
31	23849.0
32	29576.0
33	37299.0
34	56907.0
35	74012.0
36	60087.0
37	73359.0
38	102492.0
39	149990.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.94309863620152	23.118903526401304	28.81427657538822	14.123721262008957
2	31.29957144232021	25.70539348614972	29.32152582385543	13.673509247674637
3	28.729499147979777	26.04945680172555	31.394539144809468	13.826504905485198
4	25.316606185496976	29.034641274010838	30.757080825865184	14.891671714627009
5	22.848012047169654	33.2006238712855	29.764944321469212	14.186419760075633
6	22.29660494001891	41.65006029246089	26.203018585929495	9.850316181590701
7	89.84850457713189	3.0215580930598565	5.821477703111997	1.3084596266962563
8	90.104393706939	2.6358844875706953	5.664377628949439	1.5953441765408545
9	84.75563154229813	5.3147945810381625	7.311380838886089	2.618193037777614
10	40.46713919349634	32.107150742474765	14.46594466680631	12.959765397222583
11	34.462944196921406	25.034533709996094	23.81792809062551	16.684594002456986
12	32.46027208034466	22.345008746652777	26.909968919661004	18.28475025334156
13	24.64871857290859	28.74025554945397	27.841529899965465	18.769495977671973
14	20.96918007914447	30.52482747298162	28.795877467603415	19.710114980270497
15	19.768425998788487	25.297924014515484	36.05913756305233	18.8745124236437
16	22.979636433630173	23.83703485640204	34.11109664343661	19.072232066531175
17	22.873204671674998	24.37980853605376	27.843935937137328	24.90305085513392
18	24.16227446939804	24.500959584236774	30.486472409830217	20.850293536534966
19	26.183628756956278	26.051296712504033	26.12376089085649	21.641313639683197
20	27.83020737209789	26.521606213803295	26.05172130729907	19.596465106799744
21	25.948827835302506	26.941530466091862	27.864316487298957	19.245325211306678
22	25.45148579871942	24.82393469165926	27.44609061419052	22.278488895430794
23	23.595298886429383	28.052411981498988	26.843307536840676	21.50898159523095
24	22.68411845628655	25.55961593985473	31.49714955360934	20.25911605024938
25	22.616324820679466	27.005927343338676	29.124513838959686	21.253233997022175
26	21.496243751379936	29.354219623073046	26.616432384694207	22.533104240852815
27	21.25917832415265	29.499147979777966	28.111006063213672	21.13066763285571
28	20.753061328472196	28.0759062268242	30.536999190439257	20.63403325426435
29	21.349475483896533	26.545100459128506	30.417829584633065	21.687594472341896
30	22.469698084794413	27.95333986265774	29.10569013637985	20.471271916168003
31	25.8419714785523	26.958089663098185	26.584021648673286	20.615917209676233
32	26.692010258210246	26.998426168626406	26.284682318174358	20.024881254988987
33	25.237490022022318	27.105848651769993	26.446452935082288	21.210208391125402
34	22.26744943075991	27.224593662781153	28.385860427198978	22.12209647925996
35	22.07822168377312	27.735098138010294	28.454220189199443	21.732459989017148
36	25.777574601305485	27.428257632799095	26.177118303432422	20.617049462462987
37	22.871930887289896	29.377996931594947	27.386081216492393	20.36399096462276
38	22.687232151450132	29.18508936305119	26.55132784945567	21.576350636043003
39	22.34967928939815	28.806633869077608	26.908129008882526	21.935557832641717
40	24.545117442920304	26.75414262988355	26.783581202339235	21.91715872485691
41	21.244317506326464	26.070261946682216	28.44572829329876	24.23969225369256
42	22.77724624799733	26.958938852688252	26.421684905371972	23.84212999394245
43	22.27070465752184	27.369380487887724	26.94761632482068	23.412298529769757
44	21.482515186340503	28.309574895691213	27.954755178641182	22.2531547393271
45	21.15019899342727	29.68370518401938	25.98477686128205	23.181318961271295
46	21.936407022231784	29.794524425523246	26.662005559361184	21.60706299288379
47	21.699483126602846	28.1142612899756	28.18615934193468	22.000096241486876
48	22.197674352776	26.678564756367507	29.249061645502977	21.87469924535352
49	22.35534055333194	26.40455958197227	29.029829199667116	22.210270665028673
50	21.38216928311415	28.580466374922864	28.68024615175584	21.357118190207146
51	20.93167420558314	28.84555505862239	27.012154733665838	23.210616002128635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	556.0
1	850.0
2	1144.0
3	4403.0
4	7662.0
5	5122.0
6	2582.0
7	2761.0
8	2940.0
9	3284.5
10	3629.0
11	3829.0
12	4029.0
13	4041.5
14	4054.0
15	3881.0
16	3708.0
17	3373.0
18	3038.0
19	3035.0
20	3032.0
21	3076.5
22	3121.0
23	3315.5
24	3510.0
25	3945.0
26	5074.5
27	5769.0
28	6949.5
29	8130.0
30	9828.0
31	11526.0
32	13055.0
33	14584.0
34	17340.5
35	20097.0
36	22295.5
37	24494.0
38	26576.0
39	28658.0
40	33554.0
41	38450.0
42	40892.0
43	43334.0
44	46382.0
45	49430.0
46	50853.5
47	52277.0
48	52458.5
49	52640.0
50	52165.5
51	51691.0
52	48929.0
53	46167.0
54	43842.0
55	41517.0
56	39483.0
57	37449.0
58	35004.5
59	32560.0
60	29693.5
61	26827.0
62	24264.0
63	21701.0
64	18907.5
65	16114.0
66	14217.0
67	12320.0
68	10463.0
69	8606.0
70	7160.0
71	5714.0
72	4723.5
73	3733.0
74	3021.0
75	1883.5
76	1458.0
77	1186.0
78	914.0
79	672.0
80	430.0
81	291.5
82	153.0
83	106.0
84	59.0
85	40.0
86	21.0
87	19.0
88	17.0
89	14.0
90	11.0
91	9.5
92	8.0
93	5.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	706556.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.48644587331537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.77032583912484	19.804001882681334
2	5.792311143455227	3.068354711650566
3	2.609127352767845	2.073195312170164
4	1.6940800152160045	1.794806345123359
5	1.253587562243003	1.660153955740533
6	1.0486619176212937	1.6665196272290097
7	0.8593221535490131	1.5932272795401714
8	0.7901941724432161	1.674354814226118
9	0.6705111553716483	1.598351168178478
>10	10.243647814492784	59.544331959947115
>50	0.25049215861978086	3.9655541398731566
>100	0.017201178274316112	0.8244901455498962
>500	0.0	0.0
>1k	0.0	0.0
>5k	5.375368210723785E-4	0.7326586580900994
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5146	0.7283216050815505	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038213531553054535	0.0
2	0.0	0.0	0.0	0.12313249055984239	0.0
3	0.0	0.0	0.0	0.17337620797219186	0.0
4	0.0	0.0	0.0	0.29155509258997164	0.0
5	0.0	0.0	0.0	0.4990404157632233	0.0
6	0.0	0.0	0.0	0.8266860659310797	0.0
7	0.0	0.0	0.0	0.9934102887810733	0.0
8	0.0	0.0	0.0	1.3519098273880628	0.0
9	0.0	0.0	0.0	1.4992442212648396	0.0
10	0.0	0.0	0.0	1.738857217262326	0.0
11	0.0	0.0	0.0	1.9696952541624444	0.0
12	0.0	0.0	0.0	2.1698209342217742	0.0
13	0.0	0.0	0.0	2.274412785398468	0.0
14	0.0	0.0	0.0	2.325788755597575	0.0
15	0.0	0.0	0.0	2.3835336477221905	0.0
16	0.0	0.0	0.0	2.490248472874054	0.0
17	0.0	0.0	0.0	2.6020584355663243	0.0
18	0.0	0.0	0.0	2.7425993127225583	0.0
19	0.0	0.0	0.0	2.8371424204167823	0.0
20	0.0	0.0	0.0	2.935082286471278	0.0
21	0.0	0.0	0.0	3.0593470298178773	0.0
22	0.0	0.0	0.0	3.1877161895164714	0.0
23	0.0	0.0	0.0	3.3271248138859484	0.0
24	0.0	0.0	0.0	3.44445450891366	0.0
25	1.4153159834464642E-4	0.0	0.0	3.5525846500489697	0.0
26	1.4153159834464642E-4	0.0	0.0	3.6584502856107655	0.0
27	1.4153159834464642E-4	0.0	0.0	3.7607776312139447	0.0
28	1.4153159834464642E-4	0.0	0.0	3.867492456365808	0.0
29	1.4153159834464642E-4	0.0	0.0	3.9931725156958544	0.0
30	1.4153159834464642E-4	0.0	0.0	4.154093943013717	0.0
31	1.4153159834464642E-4	0.0	0.0	4.282746165909001	0.0
32	1.4153159834464642E-4	0.0	0.0	4.422154790278477	0.0
33	1.4153159834464642E-4	0.0	0.0	4.55561908751748	0.0
34	1.4153159834464642E-4	0.0	0.0	4.685120500002831	0.0
35	1.4153159834464642E-4	0.0	0.0	4.838257689411738	0.0
36	1.4153159834464642E-4	0.0	0.0	4.969032886282191	0.0
37	1.4153159834464642E-4	0.0	0.0	5.104761689094707	0.0
38	1.4153159834464642E-4	0.0	0.0	5.2693629379695315	0.0
39	1.4153159834464642E-4	0.0	0.0	5.507702149581916	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGACG	20	7.03144E-4	45.0	1
TTAACGG	40	6.8084773E-9	45.0	2
TAGGCGT	20	7.03144E-4	45.0	14
CGGGTAC	30	2.16431E-6	44.999996	6
CGGTCTA	120	0.0	44.999996	31
CACGACC	140	0.0	43.39286	27
TACGGGA	255	0.0	41.47059	4
TATTGCG	50	1.0804797E-9	40.499996	1
CGTTTTT	2540	0.0	40.482285	1
ACAACGA	95	0.0	40.263157	13
TAGGGCG	275	0.0	40.090908	5
GCGATAT	45	1.9264917E-8	40.0	9
AGGGCGA	685	0.0	39.416058	6
GGGCGAT	1305	0.0	39.137928	7
TTAGGGA	910	0.0	39.065937	4
CTAGGGA	425	0.0	38.64706	4
ACACGTC	35	6.2448707E-6	38.57143	41
TAATGCG	35	6.2448707E-6	38.57143	1
TAGGGAC	620	0.0	38.467743	5
TAACGGG	165	0.0	38.181816	3
>>END_MODULE