FastQCFastQC Report
Thu 26 May 2016
SRR1547310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547310_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2140478
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529292.4727654290303382No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC39460.18435134582088672No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG32580.1522089925708183No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA207.034653E-445.040
CGTACCG253.8919377E-545.012
ATTACGC302.1663836E-644.99999626
CGTTTTT238950.044.086631
ATACGAG900.042.5000041
CGTTCGA750.042.00000414
TAATGCG750.042.0000041
TATAACG950.040.2631571
AATCGCG451.929584E-840.0000041
CGAGCTA403.4600816E-739.37538
GACCGAA750.039.0000049
TCGTGCG750.039.0000041
CGTGCGG2100.038.571432
AGCGTTA356.250815E-638.57142633
GCGGGCT3100.038.467745
TAAGGGA33250.038.4360924
GTTTTTT278750.038.1955152
TGGGCGA9500.038.1315846
AAGGGAT37650.038.127495
AGGGATC27150.037.872936
Produced by FastQC (version 0.11.5)