##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547310_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2140478
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.352378300547823	31.0	31.0	33.0	30.0	34.0
2	31.740259885875957	31.0	31.0	34.0	30.0	34.0
3	31.66977562955564	31.0	31.0	34.0	30.0	34.0
4	35.52574938868795	37.0	35.0	37.0	33.0	37.0
5	35.47169417298379	37.0	35.0	37.0	33.0	37.0
6	35.5264665182263	37.0	35.0	37.0	33.0	37.0
7	35.95112353408911	37.0	35.0	37.0	35.0	37.0
8	35.956305554179956	37.0	35.0	37.0	35.0	37.0
9	37.71605314326987	39.0	37.0	39.0	35.0	39.0
10	37.06166192784976	39.0	37.0	39.0	33.0	39.0
11	36.734638711540136	39.0	37.0	39.0	32.0	39.0
12	36.57881323704331	39.0	35.0	39.0	32.0	39.0
13	36.48430163729784	39.0	35.0	39.0	32.0	39.0
14	37.64489427127959	40.0	36.0	41.0	32.0	41.0
15	37.735403026800554	40.0	36.0	41.0	32.0	41.0
16	37.796930872449984	40.0	36.0	41.0	33.0	41.0
17	37.690662552943785	40.0	36.0	41.0	32.0	41.0
18	37.45437093957518	39.0	36.0	41.0	32.0	41.0
19	37.28228694712116	39.0	36.0	41.0	32.0	41.0
20	37.026249277030644	38.0	35.0	41.0	32.0	41.0
21	36.926778971799756	38.0	35.0	41.0	32.0	41.0
22	36.82852475007919	38.0	35.0	40.0	31.0	41.0
23	36.73571650818182	38.0	35.0	40.0	31.0	41.0
24	36.60535123463077	38.0	35.0	40.0	31.0	41.0
25	36.528501577684985	38.0	35.0	40.0	31.0	41.0
26	36.32607763312681	38.0	35.0	40.0	31.0	41.0
27	36.28661308361964	38.0	35.0	40.0	30.0	41.0
28	36.247974050655976	38.0	35.0	40.0	30.0	41.0
29	36.230653153174195	38.0	35.0	40.0	30.0	41.0
30	36.16164800572582	38.0	35.0	40.0	30.0	41.0
31	36.00917972527632	38.0	35.0	40.0	30.0	41.0
32	35.69248644461658	38.0	35.0	40.0	28.0	41.0
33	35.321127336978	38.0	34.0	40.0	25.0	41.0
34	34.905310402629695	38.0	34.0	40.0	23.0	41.0
35	34.596991886858916	38.0	34.0	40.0	22.0	41.0
36	34.32379823572118	38.0	33.0	40.0	20.0	41.0
37	34.25717713520064	38.0	33.0	40.0	20.0	41.0
38	34.16511592270511	38.0	33.0	40.0	19.0	41.0
39	34.02400071385924	38.0	33.0	40.0	18.0	41.0
40	33.918407010022996	38.0	33.0	40.0	18.0	41.0
41	33.87216033054299	38.0	33.0	40.0	18.0	41.0
42	33.78629539757007	37.0	33.0	40.0	18.0	41.0
43	33.681048345276146	37.0	33.0	40.0	17.0	41.0
44	33.5912982987912	37.0	33.0	40.0	17.0	41.0
45	33.460468642985354	37.0	33.0	40.0	17.0	41.0
46	33.368380333738536	37.0	33.0	40.0	17.0	41.0
47	33.32231445499556	37.0	33.0	40.0	17.0	41.0
48	33.19150815845806	36.0	32.0	40.0	15.0	41.0
49	33.065456874585955	36.0	32.0	40.0	15.0	41.0
50	32.951122132533015	36.0	32.0	40.0	15.0	41.0
51	32.53136402242863	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	6.0
14	20.0
15	72.0
16	183.0
17	484.0
18	1168.0
19	2362.0
20	4174.0
21	6629.0
22	10422.0
23	15784.0
24	23955.0
25	35940.0
26	51060.0
27	56695.0
28	54492.0
29	53813.0
30	57387.0
31	66330.0
32	79701.0
33	99633.0
34	142688.0
35	192672.0
36	183771.0
37	212267.0
38	302534.0
39	486096.0
40	138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.917429658235218	23.493023520914488	25.71285479224734	20.876692028602957
2	36.39743085422975	24.642860146191644	27.24410155114886	11.715607448429743
3	25.07696878921437	24.93335600739648	37.786653261561206	12.203021941827947
4	21.9089380970045	26.091742124889862	38.56717985421948	13.43213992388616
5	20.93952846046537	29.935229420718173	36.29292148763033	12.832320631186118
6	19.93138915700138	38.118027842379135	32.903444931459234	9.047138069160253
7	82.502973634861	2.3780202366013574	14.01537413605746	1.1036319924801843
8	82.82430373028828	2.454591918253773	13.317866383116295	1.403237968341651
9	78.07260808099873	4.756834688326626	14.553711834459406	2.616845396215238
10	34.97279579607919	35.29524713638729	19.9052734949857	9.826683572547815
11	22.80144902213431	25.146999875728692	35.614708490346544	16.43684261179045
12	23.53717253809663	23.201312977755435	35.68805659296662	17.573457891181317
13	20.86860972175374	26.1012259878401	36.5788389322385	16.45132535816766
14	17.387564833649307	29.37376604664939	35.36995007657168	17.86871904312962
15	16.171528041867283	27.112869181556643	40.72898670297008	15.986616073605989
16	19.59926707959624	26.05469432528622	38.49275722525529	15.853281369862247
17	19.044951641642662	26.056376192607445	34.939345323801504	19.95932684194839
18	19.34203481652229	26.605879621280852	36.57369989320142	17.47838566899543
19	21.650070685146027	27.58799669980257	33.166423574547366	17.595509040504037
20	22.7598695244707	27.501380532759505	33.244583686447605	16.494166256322185
21	21.458477966136535	27.54482877189114	35.073707835352664	15.922985426619663
22	21.012222503571632	25.03380086130294	34.82586599815555	19.128110636969872
23	18.80453805178096	28.735777709464895	34.234829790355235	18.224854448398908
24	17.96122174579697	28.19131988275516	36.72857184236418	17.118886529083692
25	18.785430170270377	28.66401803709265	35.23231726745147	17.318234525185495
26	18.007846845424247	30.389286878912092	33.79908599854799	17.80378027711567
27	18.08357759341605	29.709485451380484	34.76312300336654	17.443813951836926
28	16.763545338938314	29.208709456485888	37.02957937432667	16.998165830249132
29	17.71235210079244	27.237000333570354	36.87760397443935	18.173043591197853
30	18.206120315181938	28.427528804313802	35.88675052955461	17.47960035094965
31	19.506016880341683	27.948897395815326	34.395728430752385	18.14935729309061
32	20.44239651143343	28.484525419088634	34.20712569809174	16.865952371386204
33	19.81538703037359	28.94881423682	32.91204114221216	18.32375759059425
34	18.428266957193674	29.44893617220079	33.203611529761105	18.91918534084443
35	18.59066059076524	29.509530114301576	33.102372460730734	18.797436834202454
36	20.479397592500366	30.237031167804574	31.13407379099435	18.14949744870071
37	18.876251005616503	31.342672057362886	31.688435947484628	18.092640989535983
38	18.76552807363589	32.05232662984623	30.012595317494505	19.169549979023376
39	18.913579116440346	31.722727353422925	30.229089016565457	19.13460451357127
40	20.099716044733935	30.840214195147063	30.613162106781754	18.446907653337245
41	18.263770989470576	30.447171145884237	30.70664589871982	20.582411965925367
42	19.348809004343888	31.284694353317345	29.89710709477042	19.469389547568348
43	19.478312788078178	30.398957616009138	30.518323477279374	19.604406118633314
44	19.32077788232348	31.38051407209044	30.312014419209167	18.986693626376912
45	18.681060959281055	31.41630047120316	29.567320944200315	20.335317625315465
46	19.418699935248107	31.63293432588422	29.61235761357977	19.336008125287904
47	19.12876469648368	30.541215560262707	31.051709010791047	19.27831073246256
48	19.362918002427495	29.95008591538899	31.21924168339969	19.467754398783825
49	18.705448035438813	29.76022178223743	31.434520700516426	20.099809481807334
50	17.974816839976864	31.467503987427108	31.109780151909995	19.447899020686034
51	17.799342016129106	31.426017926836902	30.48235020401985	20.29228985301414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1758.0
1	2307.5
2	2857.0
3	37634.0
4	72411.0
5	47620.5
6	22830.0
7	23221.0
8	23612.0
9	25565.5
10	27519.0
11	29025.5
12	30532.0
13	31094.5
14	31657.0
15	30394.5
16	29132.0
17	27157.0
18	25182.0
19	23646.5
20	22111.0
21	21681.0
22	21251.0
23	21713.0
24	22175.0
25	23783.5
26	29092.5
27	32793.0
28	37847.0
29	42901.0
30	47512.0
31	52123.0
32	57109.0
33	62095.0
34	69179.5
35	76264.0
36	80149.5
37	84035.0
38	92847.5
39	101660.0
40	111859.0
41	122058.0
42	135573.0
43	149088.0
44	162153.5
45	175219.0
46	170103.0
47	164987.0
48	156803.0
49	148619.0
50	137375.5
51	126132.0
52	117211.0
53	108290.0
54	97751.0
55	87212.0
56	79284.5
57	71357.0
58	64255.0
59	57153.0
60	50184.0
61	43215.0
62	36527.5
63	29840.0
64	24374.0
65	18908.0
66	15281.0
67	11654.0
68	9511.5
69	7369.0
70	5455.0
71	3541.0
72	2890.0
73	2239.0
74	1867.5
75	1185.5
76	875.0
77	652.0
78	429.0
79	365.0
80	301.0
81	210.0
82	119.0
83	85.5
84	52.0
85	36.0
86	20.0
87	12.0
88	4.0
89	3.5
90	3.0
91	3.5
92	4.0
93	4.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2140478.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.672343148863092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.30664073322625	21.66928233204577
2	6.985211042654759	3.8659431387914047
3	3.059306781658177	2.539745611790672
4	1.8625853629708662	2.061684052327181
5	1.2036001750243686	1.6653218528653004
6	0.8686939376605853	1.4423278040568481
7	0.6596240814134124	1.277734075108789
8	0.5278041479305593	1.168446199754219
9	0.42814561479435415	1.066301313324329
>10	4.726024100056264	30.3348901381651
>50	1.1965657555804627	23.044851931625583
>100	0.17168608851198605	6.093394256002127
>500	0.0023987708020853683	0.4505753015348008
>1k	0.0015420669441977368	0.809926055016044
>5k	0.0	0.0
>10k+	1.713407715775263E-4	2.5095759375918627
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52929	2.4727654290303382	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	3946	0.18435134582088672	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	3258	0.1522089925708183	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.041392623516803256	0.0
2	0.0	0.0	0.0	0.13674515692289294	0.0
3	0.0	0.0	0.0	0.21383074247901637	0.0
4	0.0	0.0	0.0	0.38827775851935875	0.0
5	0.0	0.0	0.0	0.849670026975283	0.0
6	0.0	0.0	0.0	1.3991267371119909	0.0
7	0.0	0.0	0.0	1.7404523662471654	0.0
8	0.0	0.0	0.0	2.2582339085008116	0.0
9	0.0	0.0	0.0	2.532331563323706	0.0
10	0.0	0.0	0.0	3.023343384047862	0.0
11	0.0	0.0	0.0	3.3235567008864377	0.0
12	0.0	0.0	0.0	3.626526411390353	0.0
13	0.0	0.0	0.0	3.7836875688514433	0.0
14	0.0	0.0	0.0	3.8734338778534516	0.0
15	0.0	0.0	0.0	3.958461614648691	0.0
16	0.0	0.0	0.0	4.079789654460359	0.0
17	0.0	0.0	0.0	4.2134513879610065	0.0
18	0.0	0.0	0.0	4.388692619125261	0.0
19	0.0	0.0	0.0	4.510674718450739	0.0
20	0.0	0.0	0.0	4.630087298257679	0.0
21	0.0	0.0	0.0	4.779960363993463	0.0
22	0.0	0.0	0.0	4.950062556120642	0.0
23	0.0	0.0	0.0	5.132872190230406	0.0
24	0.0	0.0	0.0	5.286295864755442	0.0
25	0.0	0.0	0.0	5.41995759825609	0.0
26	0.0	0.0	0.0	5.548760603939868	0.0
27	0.0	0.0	0.0	5.681814996463407	0.0
28	0.0	0.0	0.0	5.825754808038204	0.0
29	0.0	0.0	0.0	5.979318638173343	0.0
30	0.0	0.0	0.0	6.178012574761338	0.0
31	0.0	0.0	0.0	6.344797750782769	0.0
32	0.0	0.0	0.0	6.511115741437193	0.0
33	4.6718536700680875E-5	0.0	0.0	6.665567223769644	0.0
34	4.6718536700680875E-5	0.0	0.0	6.830577095396449	0.0
35	4.6718536700680875E-5	0.0	0.0	7.0068928529048184	0.0
36	4.6718536700680875E-5	0.0	0.0	7.186712500665739	0.0
37	4.6718536700680875E-5	0.0	0.0	7.358730152797646	0.0
38	4.6718536700680875E-5	0.0	0.0	7.54910818985292	0.0
39	4.6718536700680875E-5	0.0	0.0	7.780364946521291	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTA	20	7.034653E-4	45.0	40
CGTACCG	25	3.8919377E-5	45.0	12
ATTACGC	30	2.1663836E-6	44.999996	26
CGTTTTT	23895	0.0	44.08663	1
ATACGAG	90	0.0	42.500004	1
CGTTCGA	75	0.0	42.000004	14
TAATGCG	75	0.0	42.000004	1
TATAACG	95	0.0	40.263157	1
AATCGCG	45	1.929584E-8	40.000004	1
CGAGCTA	40	3.4600816E-7	39.375	38
GACCGAA	75	0.0	39.000004	9
TCGTGCG	75	0.0	39.000004	1
CGTGCGG	210	0.0	38.57143	2
AGCGTTA	35	6.250815E-6	38.571426	33
GCGGGCT	310	0.0	38.46774	5
TAAGGGA	3325	0.0	38.436092	4
GTTTTTT	27875	0.0	38.195515	2
TGGGCGA	950	0.0	38.131584	6
AAGGGAT	3765	0.0	38.12749	5
AGGGATC	2715	0.0	37.87293	6
>>END_MODULE